A simple and low-cost paper-based colorimetric method for detecting and distinguishing the GII.4 and GII.17 genotypes of norovirus

Paper-based analytical devices for virus detection: Recent strategies for current and future pandemics

“…For example, Sun et al developed a colorimetric PAD based on a simple and low-cost colorimetric assay based on a cell-free system and toehold-switch technique for detection of GII.4 and GII.17 genotypes of norovirus ( Fig. 4 B) [ 153 ]. Toehold-switch sensor is an RNA-based translational regulator, which is able to regulate protein synthesis including the enzyme β-galactosidase (LacZ).…”

“… Target Fabrication Method Detection Method Label/Biorecognition element Linear Range LOD Ref. influenza virus H1N1 RNA LFA Fluorescence fluorescent nanospheres (FNs)-DNA/streptaividin-biotin Not specified 2.02 pM [ 148 ] zika, dengue, and chikungunya virus RNA LFA Fluorescence Chitosan/hydroxylnaphthol blue (HNB) 5 -5000 copies zika virus Not specified [ 151 ] Target DNA LFA Colorimetric Chitosan/5′-digoxigenin- and 3′-biotin-labeled oligonucleotide probe Not specified 1.16×10 −15 mol [ 152 ] GII.4 and GII.17 genotypes of norovirus cutting Colorimetric Chlorophenol red-β-D-galactopyranoside (CPRG)/β-galactosidase/RNA-based riboswitch Not specified 0.5 pM and 2.6 fM [ 153 ] respiratory syncytial virus subgroups A and B cutting Colorimetric Chlorophenol red-β-D-galactopyranoside (CPRG)/β-galactosidase/RNA-based riboswitch Not specified 52 aM and 91 aM [ 154 ] hantavirus (HTNV), chikungunya fever virus (CHIKV), dengue virus (DENV), and Ebola virus (EBOV) LFA Colorimetric AuNPs/capture probe/magnetic beads Not specified 10 fM [ 156 ] SARS-CoV-2 N2 gene IVT RNA LFA Colorimetric Cas12 gRNAs/FAM-biotinylated reporter molecule Not specified 10 copies/μL [ 161 ] Norovirus DNA screen-printing and Wax printing DPV Ox-g-C 3 N 4 NPs Not specified 100 fM [ …”

Ultrasensitive and visual detection of human norovirus genotype GII.4 or GII.17 using CRISPR-Cas12a assay

“…However, real-time RT-PCR method typically relies on sophisticated equipment and highly-skilled personnel, and has an average reaction time of ~ 2 h, all of which is not suitable for simple, rapid, and point-of-care (POC) molecular assay for diagnosing NOVs infection in resource-constrained areas for routine detection. In a recent study, Sun et al presented a paper-based colorimetric method for detecting and distinguishing the GII.4 and GII.17 genotypes of NOVs [ 14 ]. However, the test by using this method needs a relatively long time (~ 3 h), and the detection range is limited between 2.6 fM and 0.5 pM, and is thus less sensitive compared with RT-PCR [ 14 ].…”

“…In a recent study, Sun et al presented a paper-based colorimetric method for detecting and distinguishing the GII.4 and GII.17 genotypes of NOVs [14]. However, the test by using this method needs a relatively long time (~ 3 h), and the detection range is limited between 2.6 fM and 0.5 pM, and is thus less sensitive compared with RT-PCR [14]. Conversely, due to the excellent rapidity, sensitivity and specificity, RNA-guided clustered nuclease-based nucleic acid detection system consisting of the regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated (Cas) nucleases, has recently shown considerable potential to exploit next-generation POC molecular diagnostics for infectious pathogens [15][16][17].…”

Desarrollo de métodos integrados para la determinación de biomarcadores genéticos

“…La longitud de la secuencia diana a amplificar está limitada a 100-250 nucleótidos (Teng et al, 2020). Esta tecnología ha sido aplicada recientemente para la detección colorimétrica de genotipos en norovirus (Sun et al, 2020). SDA (amplificación con desplazamiento de hebra) es un método de amplificación isotermo (37°C), que utiliza cuatro cebadores diferentes de los cuales uno contiene un sitio de restricción que hibrida con la secuencia de ADN (Zhang et al, 2020).…”