A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

Li, Yifan; Meng, Xiya; Guo, Degang; Gao, Jia; Huang, Qiwei; Jian, Zhang; Fischer, Reinhard; Shen, Qirong; Yu, Zhenzhong

doi:10.3390/jof8010050

Cited by 7 publications

(8 citation statements)

References 36 publications

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“…Light regulates important physiological and morphological processes of fungi 36 , 37 , 38 , 39 , 40 . Blue light can trigger photoconidiation of T. guizhouense , and this process relies on the blue light receptor BLR1 28 , 33 . The MAPK HOG signaling pathway is also involved in photoconidiation.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous study in Trichoderma guizhouense also demonstrated that the MAPK HOG1 translocates from the cytoplasm into the nucleus upon blue light stimulus, consistent with the finding in A. nidulans that SakA (HogA) accumulates in the nucleus after red light exposure 13 , 28 . Additionally, the MAPK HOG1 controls a large proportion of blue light‐regulated genes, including stress‐related genes encoding catalases, photolyases, heat shock proteins, and hydrophobins 28 , 33 .…”

Section: Introductionmentioning

confidence: 99%

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The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses in Trichoderma guizhouense

Li,

et al. 2023

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In several filamentous fungi, incident light and environmental stress signaling share the mitogen‐activated protein kinase (MAPK) HOG (SAK) pathway. It has been revealed that short‐term illumination with blue light triggers the activation of the HOG pathway in Trichoderma spp. In this study, we demonstrate the crucial role of the basic leucine zipper transcription factor ATF1 in blue light responses and signaling downstream of the MAPK HOG1 in Trichoderma guizhouense. The lack of ATF1 severely impaired photoconidiation and delayed vegetative growth and conidial germination. Upon blue light or H2O2 stimuli, HOG1 interacted with ATF1 in the nucleus. Genome‐wide transcriptome analyses revealed that 61.8% (509 out of 824) and 85.2% (702 out of 824) of blue light‐regulated genes depended on ATF1 and HOG1, respectively, of which 58.4% (481 out of 824) were regulated by both of them. Our results also show that blue light promoted conidial germination and HOG1 and ATF1 played opposite roles in controlling conidial germination in the dark. Additionally, the lack of ATF1 led to reduced oxidative stress resistance, probably because of the downregulation of catalase‐encoding genes. Overall, our results demonstrate that ATF1 is the downstream component of HOG1 and is responsible for blue light responses, conidial germination, vegetative growth, and oxidative stress resistance in T. guizhouense.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses in Trichoderma guizhouense

Li,

et al. 2023

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“…However, how Basidiomycetes sense different environmental stresses during fruiting body development is still unclear [ 49 ]. In yeasts and filamentous fungi, MAPK cascades are important signaling pathways to respond to environmental stresses and regulate processes, such as the cell cycle, reproduction, cell differentiation, morphogenesis, and stress response [ 25 , 28 , 30 , 51 , 52 , 53 , 54 ]. The signal induced by environmental stresses is transmitted by the sequential phosphorylation of a basic array of three proteins, often termed MAPKKK, MAPKK, and MAPK, [ 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…MAPK pathways are evolutionarily conserved signaling modules in eukaryotic organisms [ 25 ]. In fungi such as Saccharomyces cerevisiae and Aspergillus fumigatus , the MAPK pathways respond to a variety of environmental signals, such as cell wall stress, light, nitrogen, and carbon deprivation and high osmolarity, and regulate the expression of genes associated with cell differentiation and development [ 25 , 26 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

MAPK CcSakA of the HOG Pathway Is Involved in Stipe Elongation during Fruiting Body Development in Coprinopsis cinerea

Zhao

Yuan

Chen

et al. 2022

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Mitogen-activated protein kinase (MAPK) pathways, such as the high-osmolarity glycerol mitogen-activated protein kinase (HOG) pathway, are evolutionarily conserved signaling modules responsible for transmitting environmental stress signals in eukaryotic organisms. Here, we identified the MAPK homologue in the HOG pathway of Coprinopsis cinerea, which was named CcSakA. Furthermore, during the development of the fruiting body, CcSakA was phosphorylated in the fast elongating apical part of the stipe, which meant that CcSakA was activated in the apical elongating stipe region of the fruiting body. The knockdown of CcSakA resulted in a shorter stipe of the fruiting body compared to the control strain, and the expression of phosphomimicking mutant CcSakA led to a longer stipe of the fruiting body compared to the control strain. The chitinase CcChiE1, which plays a key role during stipe elongation, was downregulated in the CcSakA knockdown strains and upregulated in the CcSakA phosphomimicking mutant strains. The results indicated that CcSakA participated in the elongation of stipes in the fruiting body development of C. cinerea by regulating the expression of CcChiE1. Analysis of the H2O2 concentration in different parts of the stipe showed that the oxidative stress in the elongating part of the stipe was higher than those in the non-elongating part. The results indicated that CcSakA of the HOG pathway may be activated by oxidative stress. Our results demonstrated that the HOG pathway transmits stress signals and regulates the expression of CcChiE1 during fruiting body development in C. cinerea.

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“…The precise control of light conditions can likely improve the conidial yield and stress resistance to guarantee the low cost and long shelf life of Trichoderma-based biocontrol agents and biofertilizers. In a study [ 10 ], effects of wavelengths and intensities of light on conidial yield and stress tolerance to osmotic, oxidative and pH stresses in Trichoderma guizhouense were investigated. The authors found that blue light increased the conidial yield more than 1000 folds as compared to dark conditions and simultaneously enhanced conidial stress resistance.…”

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confidence: 99%

Fungal Biotechnology and Applications

2023

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A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

Cited by 7 publications

References 36 publications

The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses in Trichoderma guizhouense

The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses in Trichoderma guizhouense

MAPK CcSakA of the HOG Pathway Is Involved in Stipe Elongation during Fruiting Body Development in Coprinopsis cinerea

Fungal Biotechnology and Applications

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