A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV

Kim, Jin Hwa; Kang, Minhee; Park, Eun-Kyoung; Chung, Doo Ryeon; Kim, Jiyeon; Hwang, Eung Soo

doi:10.1007/s13206-019-3404-3

Cited by 71 publications

(48 citation statements)

References 36 publications

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“…They investigated both gel electrophoresis and real-time fluorescent methods for final RNA identification. The LOD and detection time of the assay were 10 4 copies/reaction and 20–25 min, respectively [ 68 ].…”

Section: Rna Amplification-based Detection Methodsmentioning

confidence: 99%

Laboratory detection methods for the human coronaviruses

Shabani

Dowlatshahi

Abdekhodaie

2020

Eur J Clin Microbiol Infect Dis

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Coronaviruses are a group of envelop viruses which lead to diseases in birds and mammals as well as human. Seven coronaviruses have been discovered in humans that can cause mild to lethal respiratory tract infections. HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 are the low-risk members of this family and the reason for some common colds. Besides, SARS-CoV, MERS-CoV, and newly identified SARS-CoV-2, which is also known as 2019-nCoV, are the more dangerous viruses. Due to the rapid spread of this novel coronavirus and its related disease, COVID-19, a reliable, simple, fast, and low-cost detection method is necessary for patient diagnosis and tracking worldwide. Human coronaviruses detection methods were classified and presented in this article. The laboratory detection techniques include RT-PCR, RT-LAMP, electrochemical and optical biosensors for RNA detection, and whole virus or viral proteins detection assays.

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Section: Rna Amplification-based Detection Methodsmentioning

confidence: 99%

Laboratory detection methods for the human coronaviruses

Shabani

Dowlatshahi

Abdekhodaie

2020

Eur J Clin Microbiol Infect Dis

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“…Real-time quantitative PCR was performed using the Universal SYBR Green Master Mix (Applied Biosystems, USA). cDNA was amplified using the following conditions: 95°C for 15 min followed by 40 cycles at 95°C for 30 s, 59°C for 30 s, and 72°C for 30 s. Real-time PCR analysis was performed on an Applied Biosystems StepOne system (Applied Biosystems, USA) [ 23 ]. In this study, quantification based on the relative expression of a target gene versus GAPDH gene (2 -ΔΔCt ) was employed to determine the level of mRNA expression.…”

Section: Methodsmentioning

confidence: 99%

Effect of Rosa laevigata on PM10‐Induced Inflammatory Response of Human Lung Epithelial Cells

Choi

Kim

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Particulate matter 10 (PM10) with a diameter of less than 10 mm causes inflammation and allergic reactions in the airways and lungs, which adversely affects asthmatic patients. In this study, we examined the anti-inflammatory effects of Rosa laevigata (RL), which has been previously investigated medicinally in Korea and China for the discovery of plant-derived anti-inflammatory agents with low side effects, using a PM10-induced lung inflammatory disease model. Using MTT assay, we confirmed that in A549 cells pretreated with RL, cytotoxicity induced by PM10 (100 μg/mL) exposure was attenuated. In addition, western blotting revealed that RL suppressed the expression level of MAPK/NF-κB pathways and its downstream signal, COX-2 in PM10-induced A549 cells. Moreover, real-time PCR demonstrated that RL downregulated the mRNA expression level of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-13, and IL-17) in PM10-induced A549 cells. Based on the results of this study, RL has been shown to relieve inflammation in the lungs due to PM10 exposure. Therefore, RL may be developed as a natural remedy for respiratory diseases caused by PM10 exposure.

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“…Several simultaneous investigations successfully attempted to improve the efficacy and sensitivity of SARS virus detection over a conventional nucleic acid amplification-based method and immunoassays by designing directed primer sets [58][59][60], and also by simplifying the reaction readout [59,60]. Changes in visual detections were brought about by employing calcein dye [59] or a multiplexed colorimetric reaction [60]. Remarkably, these improvements reflected upon the 'sample-to-answer' time being brought down to less than 30 min.…”

Section: Rt-lamp For the Diagnosis Of Sarsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

Augustine

Hasan

Das

et al. 2020

Biology

199

166

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The rampant spread of COVID-19 and the worldwide prevalence of infected cases demand a rapid, simple, and cost-effective Point of Care Test (PoCT) for the accurate diagnosis of this pandemic. The most common molecular tests approved by regulatory bodies across the world for COVID-19 diagnosis are based on Polymerase Chain Reaction (PCR). While PCR-based tests are highly sensitive, specific, and remarkably reliable, they have many limitations ranging from the requirement of sophisticated laboratories, need of skilled personnel, use of complex protocol, long wait times for results, and an overall high cost per test. These limitations have inspired researchers to search for alternative diagnostic methods that are fast, economical, and executable in low-resource laboratory settings. The discovery of Loop-mediated isothermal Amplification (LAMP) has provided a reliable substitute platform for the accurate detection of low copy number nucleic acids in the diagnosis of several viral diseases, including epidemics like Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). At present, a cocktail of LAMP assay reagents along with reverse transcriptase enzyme (Reverse Transcription LAMP, RT-LAMP) can be a robust solution for the rapid and cost-effective diagnosis for COVID-19, particularly in developing, and low-income countries. In summary, the development of RT-LAMP based diagnostic tools in a paper/strip format or the integration of this method into a microfluidic platform such as a Lab-on-a-chip may revolutionize the concept of PoCT for COVID-19 diagnosis. This review discusses the principle, technology and past research underpinning the success for using this method for diagnosing MERS and SARS, in addition to ongoing research, and the prominent prospect of RT-LAMP in the context of COVID-19 diagnosis.

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A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV

Abstract: suitable not only for diagnosis of clinical test, but also for surveillance of SARS virus in developing countries.

Cited by 71 publications

References 36 publications

Laboratory detection methods for the human coronaviruses

Laboratory detection methods for the human coronaviruses

Effect of Rosa laevigata on PM10‐Induced Inflammatory Response of Human Lung Epithelial Cells

Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic

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