A simple and rapid approach to the problem of tissue contamination and patient identity in histopathologic specimens

Christie, Lesley; McKay, Fiona; Purdie, Colin A.; Robertson, Katherine; Topping, David A.; Levison, David A.

doi:10.1016/j.humpath.2006.06.014

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Small specimens are now poured directly into bags before being placed into cassettes. We recently reported the use of microsatellite analysis for sorting out possible instances of specimen contamination/transposition,1 but are prompted to report on this case as it illustrates how easily such problems may arise and how potentially serious they can be.…”

Section: Discussionmentioning

confidence: 99%

“…The pathology was reviewed; this showed two tissue cores, one consisting of dense fibroconnective tissue suggestive of a pleural plaque, the other consisting of sheets of small lymphoid cells with histological appearances and immunoprofile in keeping with CLL. Due to the possibility of specimen contamination, microsatellite analysis on the DNA from each core was undertaken 1 2. The profile from each core was matched to the microsatellite profile of the patient’s BM biopsy.…”

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confidence: 99%

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A difficult diagnosis confounded by laboratory error: lessons learned

et al. 2009

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A difficult diagnosis confounded by laboratory error: lessons learned

et al. 2009

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Le dépistage néonatal de la drépanocytose en France

Bardakdjian‐Michau

2008

Archives de Pédiatrie

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Identification of Tissue Contamination by Polymorphic Deletion Probe Fluorescence In Situ Hybridization

Chiang

Yip

Betensky

et al. 2012

American Journal of Surgical Pathology

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Potential sources of error in surgical pathology include specimen misidentification, unidentified tissue, and tissue contamination of paraffin blocks and slides. Current molecular approaches to characterize unidentified or misidentified tissue include fluorescence in situ hybridization identification of sex chromosomes (XY FISH) and microsatellite analysis. Polymorphic deletion probe (PDP) FISH, a novel FISH assay based on copy number variants, can distinguish between cells and tissues from 2 individuals in situ, independent of gender. Using a panel of 3 PDPs, we compared the genotypes of potential tissue contaminants (n=19) and unidentified tissues (n=6) with patient tissues to determine the utility of PDP FISH in resolving specimen identity. XY FISH was added to increase the informative potential of the assay, and microsatellite analysis was used as a gold standard to confirm PDP FISH results. PDP FISH distinguished between putative contaminants and patient tissues in 13 of 14 cases and indicated a high likelihood of 2 tissues originating from the same source in 11 of 11 cases. The assay has a sensitivity and specificity of 86% [6/7, exact 95% confidence interval (CI): 42%, 97%] and 100% (9/9, exact 1-sided 97.5% CI: 68%, 100%), respectively, and a positive predictive value and negative predictive value of 100% (6/6, exact 1-sided 97.5% CI: 54%, 100%) and 90% (9/10, exact 95% CI: 55%, 98%), respectively. PDP FISH is an accurate and practical molecular assay for the genetic characterization of potential tissue contaminants and unidentified tissues, especially in the setting of small sample size, and permits concomitant assessment of morphology.

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A simple and rapid approach to the problem of tissue contamination and patient identity in histopathologic specimens

Cited by 3 publications

References 12 publications

A difficult diagnosis confounded by laboratory error: lessons learned

A difficult diagnosis confounded by laboratory error: lessons learned

Le dépistage néonatal de la drépanocytose en France

Identification of Tissue Contamination by Polymorphic Deletion Probe Fluorescence In Situ Hybridization

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