A Simple and Rapid Method for the Detection of HIV-1/HCV in Co-Infected Patients

Paryan, Mahdi; Forouzandeh, Mehdi; Kia, Vahid; Samira, Mohammadi Yeganeh; A, Raz; Siamak, Mirab Samiee

doi:10.5812/ijb.10717

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Higher ratio is in favor of higher risk of malignancy. The ratio more than 0.75 is considered as significant and is consistent with prostate cancer ( Figure 2 ) ( 31 , 32 ).…”

Section: Resultsmentioning

confidence: 93%

MRI in Prostate Cancer

Ghafoori

Alavi

Ghasabeh

2013

Iran Red Crescent Med J

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Imaging studies play an important role in detection and management of prostate cancer and MRI especially with the use of endorectal coil because of high contrast resolution is recognized as the best imaging modality in evaluation of prostate cancer. Multiparametric MR study including T1 and T2 weighted images, diffusion weighted images, dynamic contrast study and MR spectroscopy is useful for detection and local staging of prostate cancer as well as posts treatment evaluation of patients either after surgery or radiation therapy for detection of local recurrence.

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“…Higher ratio is in favor of higher risk of malignancy. The ratio more than 0.75 is considered as significant and is consistent with prostate cancer ( Figure 2 ) ( 31 , 32 ).…”

Section: Resultsmentioning

confidence: 93%

MRI in Prostate Cancer

Ghafoori

Alavi

Ghasabeh

2013

Iran Red Crescent Med J

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“…A multiplex NASBA assay was used for simultaneous detection of HIV-1 and HCV in plasma samples. The LoD of the assay for both HIV-1 and HCV was determined to be 1000 copies/ml and no cross-reactions with other selected viruses [ 45 ]. Swenson and his colleagues [ 75 ] used real-time TMA for the detection of HSV-1 and HSV-2 in lesion swab specimens with overall sensitivities of 98.2% and 99.4%, respectively, and specificity of 97.8% and 94.5%, respectively, compared to culture.…”

Section: Molecular Diagnostic Techniques Of Medical Virusesmentioning

confidence: 99%

Molecular and Immunological Diagnostic Techniques of Medical Viruses

Reta

Tessema

Ashenef

et al. 2020

International Journal of Microbiology

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Viral infections are causing serious problems in human population worldwide. The recent outbreak of coronavirus disease 2019 caused by SARS-CoV-2 is a perfect example how viral infection could pose a great threat to global public health and economic sectors. Therefore, the first step in combating viral pathogens is to get a timely and accurate diagnosis. Early and accurate detection of the viral presence in patient sample is crucial for appropriate treatment, control, and prevention of epidemics. Here, we summarize some of the molecular and immunological diagnostic approaches available for the detection of viral infections of humans. Molecular diagnostic techniques provide rapid viral detection in patient sample. They are also relatively inexpensive and highly sensitive and specific diagnostic methods. Immunological-based techniques have been extensively utilized for the detection and epidemiological studies of human viral infections. They can detect antiviral antibodies or viral antigens in clinical samples. There are several commercially available molecular and immunological diagnostic kits that facilitate the use of these methods in the majority of clinical laboratories worldwide. In developing countries including Ethiopia where most of viral infections are endemic, exposure to improved or new methods is highly limited as these methods are very costly to use and also require technical skills. Since researchers and clinicians in all corners of the globe are working hard, it is hoped that in the near future, they will develop good quality tests that can be accessible in low-income countries.

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“…Commercial NASBA assays are clinically used for the detection of RSV [38], HIV-1 [39], CMV [40] and human papillomavirus (HPV) [41]. A multiplex NASBA assay for HIV-1 and HCV has been developed and demonstrated an analytical sensitivity of 1000 copies/mL, 93.3% clinical sensitivity, and 100% specificity [42].…”

Section: Nucleic Acid Sequence Based Amplification (Nasba)mentioning

confidence: 99%

Amplification chemistries in clinical virology

Dunbar

Das

2019

Journal of Clinical Virology

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Molecular diagnostic methods have evolved and matured considerably over the last several decades and are constantly being evaluated and adopted by clinical laboratories for the identification of infectious pathogens. Advancement in other technologies such as fluorescence, electronics, instrumentation, automation, and sensors have made the overall diagnostic process more accurate, sensitive, and rapid. Nucleic acid based detection procedures, which rely on the fundamental principles of DNA replication have emerged as a popular and standard diagnostic method, and several commercial assays are currently available based on different nucleic acid amplification techniques. This review focuses on the major amplification chemistries that are used for developing commercial assays and discusses their application in the clinical virology laboratory. Polymerase chain reaction (PCR)The development of PCR by Kary Mullis in the 1980s revolutionized the detection of microbial pathogens in clinical specimens. PCR is a highly sensitive method that involves cyclical heating and cooling of the reaction mixture to facilitate amplification of the target DNA [3]. The method consists of three basic steps (denaturation, annealing, and extension) and the reaction mixture requires four primary components (template DNA, primers, nucleotides, and a DNA polymerase) (Fig. 1a). Double-stranded target DNA (dsDNA) is thermally denatured to form a single-stranded DNA template (ssDNA). Oligonucleotide primers anneal to complementary target sequences on the nucleic acid template and are extended by the DNA polymerase, thus creating the resultant PCR product (amplicon). The reaction occurs in a programmable thermal https://doi.

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A Simple and Rapid Method for the Detection of HIV-1/HCV in Co-Infected Patients

Cited by 4 publications

References 23 publications

MRI in Prostate Cancer

MRI in Prostate Cancer

Molecular and Immunological Diagnostic Techniques of Medical Viruses

Amplification chemistries in clinical virology

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