A simple and rapid method for isolation of serum lipoproteins from the other serum protein constituents

Wille, Leif E.

doi:10.1016/0009-8981(76)90554-4

Cited by 22 publications

(4 citation statements)

References 2 publications

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“…BSA and other reagents were purchased from Sigma. XSP were obtained by the passage of normal male serum over a column of Affi-Gel Blue (Bio-Rad) to remove the albumin (12). The resulting protein mixture was dialyzed against distilled water and lyophilized.…”

Section: Methodsmentioning

confidence: 99%

Proteins iodinated by the Chloramine-T method appear to be degraded at an abnormally rapid rate after endocytosis

Opresko

Wiley

Wallace

1980

Proc. Natl. Acad. Sci. U.S.A.

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Proteins iodinated by the chloramine-T method appear to be degraded at an abnormally rapid rate after endocytosis ( Proteins labeled with either 3H by reductive methylation or 125I by the chloramine-T method were incubated with Xenopus laevis oocytes; the incorporation and acid precipitability of the proteins were then studied. The uptake rates of both specifically incorporated (vitellogenin) and nonspecifically incorporated proteins (bovine serum albumin and X.

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Section: Methodsmentioning

confidence: 99%

Proteins iodinated by the Chloramine-T method appear to be degraded at an abnormally rapid rate after endocytosis

Opresko

Wiley

Wallace

1980

Proc. Natl. Acad. Sci. U.S.A.

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“…This isolated system offers the unique opportunity to examine the vascular carrier in greater detail than is possible in intact-animal experiments. Moreover, we have utilized affinity chromatography on Blue Sepharose CL-6B (Travis & Pannell, 1973; Wille, 1976) to fractionate the serum proteins in the vascular effluent of the perfused intestine. This method of (UM-20) were obtained from Amicon Corp., Lexington, MA, U.S.A.…”

mentioning

confidence: 99%

Identification of albumin as the plasma carrier for zinc absorption by perfused rat intestine

Smith¹,

Failla²,

Cousins³

1979

Biochemical Journal

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The isolated vascularly perfused rat intestine exhibits an obligatory need for a protein carrier in order to absorb zinc. Therefore this system is ideal for use as a model to identify the plasma carrier during zinc absorption. Affinity chromatography on Blue Sepharose CL-6B was employed to separate the major serum zinc-binding proteins in the portal effluent of the perfused intestine. It was found that 94% of newly absorbed 65Zn was transported in the portal serum-containing perfusate as an albumin-65Zn complex. The identity of albumin as the plasma carrier was confirmed by polyacrylamide-slab-gel electrophoresis. This evidence suggests that albumin is the plasma protein that is involved in removal of zinc from intestinal-mucosal cells and subsequent transport of the metal in portal blood to the liver.

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“…The eluates, designated Fraction 1 and Fraction I1 respectively, were concentrated by dialysis against saline. Fraction I contains all the serum globulins, whereas the lipoproteins and albumin were almost quantitatively recovered in Fraction 11 [27].…”

Section: A T E R I a L A N D M E T H O D Smentioning

confidence: 96%

Studies on the pre-α-lipoprotein of human serum: I. Characterization of an albumin-containing lipoprotein

Wille

1977

Scandinavian Journal of Clinical and Laboratory Investigation

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In this study an albumin-containing lipoprotein is isolated by column affinity chromatography and electrophoresis. Its electrophoretic mobility is faster than that of albumin in agarose gel. Immunologically it reacts with anti-albumin and anti-apolipoprotein-A-I. No reaction is obtained against antibodies to any of the other previously known lipoportein families: Apo-AII, Apo-B, Lp(a), Apo-C, LpX, Apo-D or Apo-E ('the arginine rich peptide'). It differs from purified albumin in having higher amounts of glycine, serine and glutamic acid. Moreover, the molecular weight is estimated to 80,000 as compared to 67,000 for albumin.

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A simple and rapid method for isolation of serum lipoproteins from the other serum protein constituents

Cited by 22 publications

References 2 publications

Proteins iodinated by the Chloramine-T method appear to be degraded at an abnormally rapid rate after endocytosis

Proteins iodinated by the Chloramine-T method appear to be degraded at an abnormally rapid rate after endocytosis

Identification of albumin as the plasma carrier for zinc absorption by perfused rat intestine

Studies on the pre-α-lipoprotein of human serum: I. Characterization of an albumin-containing lipoprotein

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