A simple and rapid method for screening amylolytic bacteria

Abstract

A laboratory class was designed for the study of the ecology of amylolytic bacteria in soil, although other sources may be equally suitable for this purpose. Groups of three students carried out the following: (a) preparation and sterilization of medium and plates, (b) collection and preparation of soil samples, spreading the samples on the plates, (c) incubation of the plates at 373C overnight, a further 1 h incubation at 603C to observe amylolytic activity due to thermophilic bacteria, and (d) interpretation and discussion of the results. These tasks are accomplished in two periods of 4 h on consecutive days. No sophisticated instruments are required for these experiments, which can be carried out in three classes of 4 h each. On the "rst day the students prepare culture media, bu!ers and reagents, as well as collect and grow soil samples. The second day is spent for both taxonomic identi"cation of colonies and the HAI determination.

Characterization of the activity and stability of amylase from saliva and detergent: Laboratory practicals for studying the activity and stability of amylase from saliva and various commercial detergents

“…The cited article presents a methodological approach and suggests possible studies regarding incubation times, enzyme and substrate concentrations, and the effect of inhibitors. Effio et al [23] presented a methodology for screening amylolytic bacteria using agar plates and the dye Cibachron Brilliant Blue FR, in which the ratio between colony diameter and the diameter of the halo of amylolytic activity was measured. Fernandes et al [24] used a histopathological technique known as periodic acid and Schiff reactive (PAS) staining to detect sugar metabolic and tumor diseases.…”

Molecular expression of a recombinant thermostable bacterial amylase from Geobacillus stearothermophilus SR74 using methanol-free Meyerozyma guilliermondii strain SO yeast system

“…Preliminary screening of α-amylase was completed using a method from a previous study (Effio et al 2000). Wild-type M. guilliermondii strain SO was cultured on YPD agar containing 1% (w/v) starch for 2 to 3 days.…”

“…Preliminary screening of α-amylase was performed according to a previous study (Effio et al 2000). Colony 23 of M. guilliermondii strain SO containing recombinant plasmid, wild-type SO, and P. pastoris KM71H containing empty plasmid (negative control) were cultured on YPD (1% yeast extract, 2% peptone, 2% dextrose, and 2% agar) agar containing 1% (w/v) starch for 3 days.…”