2004
DOI: 10.1016/j.jviromet.2004.01.016
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A simple and rapid single-step multiplex RT-PCR to detect Norovirus, Astrovirus and Adenovirus in clinical stool samples

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Cited by 80 publications
(44 citation statements)
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“…For second-round amplification, master mix composition and cycling conditions were identical to those used for first-round PCR, except that primers GeneRacer 5Ј-nested (5Ј-GGACACUGACAUGGACUGAAGGAGUA-3Ј) and 140-NV-ORF3 (5Ј-GCTCTCAACACATCATTTGTCACC-3Ј) were used. Amplicons were sequenced as previously described (18,20). Sequences were generated in both directions for each amplified viral nucleic acid.…”
Section: Methodsmentioning
confidence: 99%
“…For second-round amplification, master mix composition and cycling conditions were identical to those used for first-round PCR, except that primers GeneRacer 5Ј-nested (5Ј-GGACACUGACAUGGACUGAAGGAGUA-3Ј) and 140-NV-ORF3 (5Ј-GCTCTCAACACATCATTTGTCACC-3Ј) were used. Amplicons were sequenced as previously described (18,20). Sequences were generated in both directions for each amplified viral nucleic acid.…”
Section: Methodsmentioning
confidence: 99%
“…2B). Thus, to define the stage at which it inhibits viral replication, IFN-␤-pretreated Caco2 cells were infected with HAstV-1 (MOI of 5), RNA was collected at 1, 4, 8, and 16 hpi, and negative-and positive-strand RNA synthesis was monitored by RT-PCR and quantified using ImageJ using ␤-actin levels to standardize as described previously (24,25,38). IFN-␤ pretreatment did not impact RNA levels at 1 and 4 hpi, suggesting that IFN-␤ pretreatment has no effect on HAstV-1 entry (Fig.…”
Section: Experiments Performed At Least Three Times (A To C) or Twicementioning
confidence: 99%
“…Stool samples were collected from children under 4 years of age presenting with acute gastroenteritis in 20 pediatric practices from five German cities over a 14-month period (34). In the first step, NV RNA extraction and amplification were performed as described previously (43). Those samples testing positive for NV were selected for complete capsid sequence characterization.…”
Section: Viral Strainsmentioning
confidence: 99%