A simple and reliable procedure for cord blood banking, processing, and freezing: St Louis and Ohio Cord Blood Bank experiences

Alonso, J.M.F.; Regan, Donna; Johnson, C.E.; Oliver, Dana; Fegan, R. L.; Lasky, LC; Wall, D.

doi:10.1080/146532401317248036

Cited by 39 publications

(48 citation statements)

References 8 publications

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“…A number of methods of processing CB units have been developed, which generally include RBC reduction. However, all RBC reduction processing methods lose significant numbers of hematopoietic progenitor cells 12,13,15,20‐22 . An alternative method of processing is by PDR wherein only a portion of the plasma is removed from the CB unit, which results in less than 0.1% TNC loss (mean, 0.072%; median, 0.055%; range, 0%‐0.21%) 17 and more than 90% recovery when taking into account sampling for infectious disease testing, HLA typing, and so forth and for archiving purposes.…”

Section: Discussionmentioning

confidence: 99%

Analysis of 120 pediatric patients with nonmalignant disorders transplanted using unrelated plasma‐depleted or ‐reduced cord blood

et al. 2011

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Section: Discussionmentioning

confidence: 99%

Analysis of 120 pediatric patients with nonmalignant disorders transplanted using unrelated plasma‐depleted or ‐reduced cord blood

et al. 2011

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“…CB was collected via cannulation of the umbilical vein into a sterile anticoagulated (CPD) blood bag with the placenta in situ under institutional review board approval and donor informed consent, which allows use of CB products for research if the TNC content of the product is less than 1 × 10 9 total cells. Collected CB units were shipped at room temperature (22‐24°C, with temperature monitoring) to a central processing laboratory where all processing, cryopreservation, and product characterization was performed as previously described 13,14 …”

Section: Methodsmentioning

confidence: 99%

Factors influencing cord blood viability assessment before cryopreservation

et al. 2010

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CB cell populations exhibit differential time- and temperature-dependent susceptibility to in vitro cell death; consequently, global viability measurements using TB, AO/PI, or 7-AAD (Tot-AAD) significantly underestimate (4-24 degrees C) or overestimate (24-37 degrees C) CD34+ viability and CFC recovery. Our results demonstrate the limitations of global viability assessment with TB, AO/PI, and total AAD; endorse the routine use of CD34+ cell viability measurements; emphasize the importance of temperature control during shipment; and have implications with regard to establishing acceptable "cutoff" values for viability measurements and CB collection through processing time.

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“…On January 1, 2014, processing limits were raised to 15 9 10 8 for donors of Caucasian origin and to 12.5 9 10 8 for donors with both parents of non-Caucasian origin. A volume-reduction step is performed using a Sepax instrument (Biosafe, Eysins, Switzerland) after the addition of 20 % hydroxyethyl starch (Grifols, Los Angeles, USA) relative to the volume of the unit (Alonso et al 2001;Rubinstein et al 1994). The result is a standardized volume of 21 ml CB buffy-coat (CBBC) in a storage bag divided into one main and one smaller accessory compartment (MacoPharma, Turcoing, France).…”

Section: Cord Blood Collectionmentioning

confidence: 99%

High quality cord blood banking is feasible with delayed clamping practices. The eight-year experience and current status of the national Swedish Cord Blood Bank

et al. 2016

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The National Swedish Cord Blood Bank (NS-CBB) is altruistic and publicly funded. Herein we describe the status of the bank and the impact of delayed versus early clamping on cell number and volume. Cord Blood Units (CBUs) were collected at two University Hospitals in Sweden. Collected volume and nucleated cell content (TNC) were investigated in 146 consecutive Cord Blood (CB) collections sampled during the first quarter of 2012 and in 162 consecutive CB collections done in the first quarter of 2013, before and after clamping practices were changed from immediate to late (60 s) clamping. NS-CBB now holds close to 5000 units whereof 30 % are from non-Caucasian or mixed origins. Delayed clamping had no major effect on collection efficiency. The volume collected was slightly reduced (mean difference, 8.1 ml; 95 % CI, 1.3-15.0 ml; p = 0.02), while cell recovery was not (p = 0.1). The proportion of CBUs that met initial total TNC banking criteria was 60 % using a TNC threshold of 12.5 × 10(8), and 47 % using a threshold of 15 × 10(8) for the early clamping group and 52 and 37 % in the late clamping group. Following implementation of delayed clamping practices at NS-CBB; close to 40 % of the collections in the late clamping group still met the high TNC banking threshold and were eligible for banking, implicating that that cord blood banking is feasible with delayed clamping practices.

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A simple and reliable procedure for cord blood banking, processing, and freezing: St Louis and Ohio Cord Blood Bank experiences

Cited by 39 publications

References 8 publications

Analysis of 120 pediatric patients with nonmalignant disorders transplanted using unrelated plasma‐depleted or ‐reduced cord blood

Analysis of 120 pediatric patients with nonmalignant disorders transplanted using unrelated plasma‐depleted or ‐reduced cord blood

Factors influencing cord blood viability assessment before cryopreservation

High quality cord blood banking is feasible with delayed clamping practices. The eight-year experience and current status of the national Swedish Cord Blood Bank

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