A simple and robust high-performance liquid chromatography coupled to a diode-array detector method for the analysis of genistein in mouse tissues

Abstract

A simple liquid-liquid extraction procedure and quantification by high-performance liquid chromatography (HPLC) method coupled to a diode-array detector (DAD) of genistein (GEN) was developed in various mouse biological matrices. 7-ethoxycoumarin was used as internal standard (IS) and peaks were optimally separated using a Kinetex C18 column (2.6 µm, 150 mm X 2.10 mm I.D.) at 40 ºC with an isocratic elution of mobile phase with sodium dihydrogen phosphate 0.01M in water at pH 2.5 and methanol (55:45, v/v), at a flow rate of 0.25 mL/min.The injection volume was 10 µL. In all cases, the range of GEN recovery was higher than 61%.The low limit of quantification (LLOQ) was 25 ng/mL. The linearity of the calibration curves was satisfactory in all cases as shown by correlation coefficients >0.996. The within-day and between-day precisions were <15% and the accuracy ranged in all cases between 90.14 and 106.05%. This method was successfully applied to quantify GEN in liver, spleen, kidney and plasma after intravenous administration of a single dose (30 mg/Kg) in female BALB/C mice.

Improving the genistein oral bioavailability via its formulation into the metal–organic framework MIL-100(Fe)

“…, respectively). 57 Aside from the increased drug saturation in GEN@MIL-100 NPs by the formation of the calcium salt of GEN, the probable adherence of the GEN@MIL-100 NPs to the intestinal mucosa (as previously observed in MIL-127 MOF) 58 , t 1/2 = 7.5 h for intravenous administered polyethylenglycol modified copolymer micelles (MePEG-PLGA), 59 and AUC = 7500 ng•h•mL -1…”

A new Approach for the Voltammetric Sensing of the Phytoestrogen Genistein at a Non‐modified Boron‐doped Diamond Electrode

“…In comparison to existing methods for determination genistein, including HPLC [23], gas chromatography (GC) [24], capillary electrophoresis (CE) [25], as well as so on, the electrochemical approach is less time‐consuming, simpler, as well as more convenient. In the literature, there were four voltammetric studies for determination of genistein.…”