A simple and sensitive method for l-cysteine detection based on the fluorescence intensity increment of quantum dots

Huang, Shan; Li, Ran; Guan, Hongliang; Liu, Jing; Liu, Xiaorong; He, Zhike; Liu, Yi

doi:10.1016/j.aca.2009.04.034

Cited by 102 publications

(30 citation statements)

References 38 publications

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“…QDs exhibit a lot of important advantages in many aspects, including high quantum yield of fluorescence, broad excitation spectrum and narrow/symmetric emission spectrum, high level of brightness as well as excellent photochemical stability as compared with traditional organic dyes and fluorescent proteins [6][7][8][9]. Therefore, QDs capped with different reagents have been widely used in chemical and biological assay [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

A novel method for iodate determination using cadmium sulfide quantum dots as fluorescence probes

Tang

Lin

et al. 2010

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

A novel method for iodate determination using cadmium sulfide quantum dots as fluorescence probes

Tang

Lin

et al. 2010

Analytica Chimica Acta

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“…The sensitivity of this porous silicon/ethanol probe to L-Cys is lower than some of the alternative approaches [11,25]. However, in the present study, we firstly fabricated a new method for the detection of L-Cys by using porous SiNPs in ethanol as a fluorescence “off-on” probe, which will broadly benefit the development of a new optical label-free biosensor based on porous nanoparticle materials.…”

Section: Resultsmentioning

confidence: 98%

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

Zhang

Jia

2017

Sensors

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A new type of fluorescence “off-on” probe was designed for L-Cysteine (L-Cys) based on the fluorescence resonance energy transfer (FRET) between negatively charged amino-capped porous silicon nanoparticles (SiNPs) and positively charged citrate-stabilized Au nanoparticles (AuNPs). In this proposed FRET immunosensor, novel water-soluble amino-conjugated porous SiNPs in ethanol with excellent photoluminescence properties act as the energy donor. Excellent quenching efficiency between SiNPs-ethanol and citrate-stabilized AuNPs by electrostatic interaction via FRET provides an ideal “off-state” (turn-off). The addition of L-Cys leads to releasing the adsorbed AuNPs from the surface of SiNPs and hence the fluorescence emission of SiNPs-ethanol is restored (turn-on), which means the coordination ability of the thiols with AuNPs is stronger than that of the electrostatic interaction. The fluorescence intensity of SiNPs-AuNPs in ethanol is sensitive to L-Cys, and such a restored fluorescence is proportional to the concentration of L-Cys. The method will broadly benefit the development of a new thiol biosensor based on nanostructured porous materials, and the proposed procedure is also expected to develop a variety of functional nanoparticles to form other novel kinds of nanosensors.

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“…The reason for the fluorescence decay may be attributed to the existence of ions in solution. [19][20][21] The existence of ions in solution may lead to the fluorescence of hydrocortisone antigen-QDs quenching. Thus, the incubated time was fixed to 2 h.…”

Section: Effect Of the Incubation Time On Fluorescence Intensitymentioning

confidence: 99%

Determination of Hydrocortisone in Cosmetics by CdSe/CdS Quantum Dots‐Based Fluoroimmunoassay Using Magnetic Fe₃O₄/Au Nanoparticles as Solid Carriers

Wang¹,

Lu²,

Tao³

et al. 2011

Chin. J. Chem.

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This work demonstrated the feasibility of detecting hydrocortisone in cosmetics using a novel CdSe/CdS quantum dots-based competitive fluoroimmunoassay with magnetic core/shell Fe 3 O 4 /Au nanoparticles (MCFN) as solid carriers. Hydrocortisone antigen was labeled with the synthesized core/shell CdSe/CdS quantum dots (QDs) to form the antigen-QDs conjugate. Meanwhile, hydrocortisone antibody was incubated with MCFN and the immobilized antibody was obtained. The immobilized antibody was then mixed sequentially with hydrocortisone and a slightly excess amount of the QDs-labeled hydrocortisone antigen, allowing their competition for binding with the antibody immobilized on MCFN. The bound hydrocortisone and the antigen-QDs conjugates on MCFN were removed subsequently after the mixture was applied to a magnetic force. The analyte concentration was obtained by measuring the fluorescence intensity of the unbound hydrocortisone antigen-QDs conjugates. The proposed method was characterized by simplicity, rapidity, and high sensitivity with a wide linear working range of 0.5 to 15000 pg•mL -1 and a low detection limit of 0.5 pg•mL -1 . The proposed method was successfully applied to the determination of hydrocortisone in cosmetics with satisfactory results.

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A simple and sensitive method for l-cysteine detection based on the fluorescence intensity increment of quantum dots

Cited by 102 publications

References 38 publications

A novel method for iodate determination using cadmium sulfide quantum dots as fluorescence probes

A novel method for iodate determination using cadmium sulfide quantum dots as fluorescence probes

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

Determination of Hydrocortisone in Cosmetics by CdSe/CdS Quantum Dots‐Based Fluoroimmunoassay Using Magnetic Fe₃O₄/Au Nanoparticles as Solid Carriers

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A simple and sensitive method for l-cysteine detection based on the fluorescence intensity increment of quantum dots

Cited by 102 publications

References 38 publications

A novel method for iodate determination using cadmium sulfide quantum dots as fluorescence probes

A novel method for iodate determination using cadmium sulfide quantum dots as fluorescence probes

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

Determination of Hydrocortisone in Cosmetics by CdSe/CdS Quantum Dots‐Based Fluoroimmunoassay Using Magnetic Fe3O4/Au Nanoparticles as Solid Carriers

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Determination of Hydrocortisone in Cosmetics by CdSe/CdS Quantum Dots‐Based Fluoroimmunoassay Using Magnetic Fe₃O₄/Au Nanoparticles as Solid Carriers