A simple and smart telemedicine device for developing regions: a pocket-sized colorimetric reader

Lee, Dae-Sik; Jeon, Byoung Goo; Ihm, Chunhwa; Park, Je‐Kyun; Jung, Mun Yeon

doi:10.1039/c0lc00209g

Cited by 104 publications

(80 citation statements)

References 14 publications

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“…For example, urine can be colorimetrically tested for glucose, protein, nitrites, bilirubin, urobilinogen, ketones, erythrocytes, and leukocytes. 17 We specifically use the prototype developed by wax printing to test protein and glucose levels within a human urine sample. The design of the prototype μPAD requires very small sample sizes; in the case of a urine sample, 3 μL is all that is necessary.…”

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confidence: 99%

Three-Dimensional Paper-Based Microfluidic Device for Assays of Protein and Glucose in Urine

et al. 2013

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The first step in curing a disease is being able to detect the disease effectively. Paper-based microfluidic devices are biodegradable and can make diagnosing diseases costeffective and easy in almost all environments. We created a three-dimesnional (3D) paper device using wax printing fabrication technique and basic principles of origami. This design allows for a versatile fabrication technique over previously reported patterning of SU-8 photoresist on chromatography paper by employing a readily available wax printer. The design also utilizes multiple colorimetric assays that can accommodate one or more analytes including urine, blood, and saliva. In this case to demonstrate the functionality of the 3D paper-based microfluidic system, a urinalysis of protein and glucose assays is conducted. The amounts of glucose and protein introduced to the device are found to be proportional to the color change of each assay. This color change was quantified by use of Adobe Photoshop. Urine samples from participants with no pre-existing health conditions and one person with diabetes were collected and compared against synthetic urine samples with predetermined glucose and protein levels. Utilizing this method, we were able to confirm that both protein and glucose levels were in fact within healthy ranges for healthy participants. For the participant with diabetes, glucose was found to be above the healthy range while the protein level was in the healthy range. D iseases surround the world and being able to diagnose them is crucial in order to then cure the disease. Diagnosing diseases is not always an affordable, time-efficient, or easy task. Paper-based microfluidic devices construct a framework that leaves the level of complication and cost low while keeping the efficiency high.1−6 The application of paperbased microfluidics presents many advantages such as their cost effectiveness: they are easy to mass-produce, transport, store, implement, dispose, and they do not need excessive equipment to move liquid like other devices. There are also many disadvantages to paper-based microfluidic devices. They are relatively new, which means issues such as the control over flow rates, mixing, and interaction times between sample and reagents have not yet been perfected. 7−9A previous approach by Liu and Crooks 10 uses photolithography and origami to develop a 3D paper-based analytical device (μPAD). The inclusion of origami to the overall design of the μPAD allows for only one step of the fabrication process. The μPAD can be simply folded into multiple layers. This also eliminates the contamination and nonspecific adsorption caused by using tape in previous 3D designs. 3 Following the procedure, analysis can be done by unfolding the μPAD, revealing the colorimetric and fluorescence assays on each layer. Photolithography patterns the paper by use of a light-sensitive chemical as photoresist, creating a pattern on the paper to control fluid flow without any input of excess energy such as a pump or valve system in glass-based ...

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confidence: 99%

Three-Dimensional Paper-Based Microfluidic Device for Assays of Protein and Glucose in Urine

et al. 2013

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“…To date, the use of smartphone cameras to interpret lateral flow tests has focused on individual still image end‐point readings to interpret paper‐based tests for diagnostics,15, 16, 17, 18, 19, 20, 21, 22, 23 chemical sensing,24, 25 and drug monitoring 26, 27. Cameras and smartphones have also been used with other microfluidic techniques to quantify biological reactions, such as the detection of nucleic acid sequences28 and E. coli detection using quantum dots 29.…”

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Quantifying Biomolecular Binding Constants using Video Paper Analytical Devices

Miller

Parolo

Turbé

et al. 2018

Chemistry A European J

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A novel ultra‐low‐cost biochemical analysis platform to quantify protein dissociation binding constants and kinetics using paper microfluidics is reported. This approach marries video imaging with one of humankind's oldest materials: paper, requiring no large, expensive laboratory equipment, complex microfluidics or external power. Temporal measurements of nanoparticle–antibody conjugates binding on paper is found to follow the Langmuir Adsorption Model. This is exploited to measure a series of antibody–antigen dissociation constants on paper, showing excellent agreement with a gold‐standard benchtop interferometer. The concept is demonstrated with a camera and low‐end smartphone, 500‐fold cheaper than the reference method, and can be multiplexed to measure ten reactions in parallel. These findings will help to widen access to quantitative analytical biochemistry, for diverse applications spanning disease diagnostics, drug discovery, and environmental analysis in resource‐limited settings.

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“…To avoid subjective interpretation of the color, human eye is substituted for smartphone high resolution built-in camera, used as a colorimetric sensor [28]. A digital image of the colorimetric paper is acquired by the camera and can be either analyzed directly or transferred to a healthcare facility via a network platform for further processing [22,29]. Basically, the paper color is described as a linear combination of three filters defining the red, green and blue channels (RGB) of the camera.…”

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confidence: 99%

Two low-cost digital camera-based platforms for quantitative creatinine analysis in urine

Debus

Kirsanov

Yaroshenko

et al. 2015

Analytica Chimica Acta

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h i g h l i g h t s g r a p h i c a l a b s t r a c tDescription of two homemade platforms for the cheap quantification of creatinine level in urine. Image processing to extract absorption spectra and spectral fingerprints. PLS regression for creatinine evaluation in urine from digital camera images.Validation of results with capillary electrophoresis reference data.a r t i c l e i n f o . Good repeatability was observed for intra-day (1.7e2.9%) and inter-day (3.6e6.5%) measurements evaluated on three consecutive days. The performance of CDS and CSPT was also validated in real human urine samples (n ¼ 26) using capillary electrophoresis data as reference. Corresponding Partial Least-Squares (PLS) regression models provided for mean relative errors below 10% in creatinine quantification.

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A simple and smart telemedicine device for developing regions: a pocket-sized colorimetric reader

Cited by 104 publications

References 14 publications

Three-Dimensional Paper-Based Microfluidic Device for Assays of Protein and Glucose in Urine

Three-Dimensional Paper-Based Microfluidic Device for Assays of Protein and Glucose in Urine

Quantifying Biomolecular Binding Constants using Video Paper Analytical Devices

Two low-cost digital camera-based platforms for quantitative creatinine analysis in urine

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