A Simple Apparatus for Individual C. elegans Culture

Automated identification and registration of anatomical landmarks inC. elegans

“…Using longitudinal brightfield and fluorescent image timeseries acquired using in our Worm Corral system, we selected images from 3–7 days post hatch (corresponding to young through mid-adulthood), resulting in a dataset of 6527 individual C. elegans images (36, 37) (See Methods). We defined three sets of image pairs to compare: lab-frame vs. worm-frame images; consecutive lab-frame images of the same individual; and random pairs of lab-frame images from different individuals.…”

“…We obtained VT1072 ( Plin-4 ::GFP) animals from the Caenorhabditis Genetics Center (CGC), which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440), and crossed them into the temperature-sensitive sterile mutant BA671 (spe-9(hc88)) (47). Longitudinal brightfield timeseries images of C. elegans animals were acquired using the single- animal culturing system described by Pittman et al (36). Brightfield and fluorescent images were acquired in succession every 3-6 hours from hatching until death.…”

“…Timepoint images were taken from multiple experiments where animals were cultured at 20°C or 25°C in the worm corral. At 20°C, reproduction was prevented through the use of pos-1 RNAi (36, 48). For worms cultured at 25°C, the spe-9 mutation prevents fertilization.…”

“…Longitudinal brightfield timeseries images of C. elegans animals were acquired using the singleanimal culturing system described by Pittman et al (36). Brightfield and fluorescent images were acquired in succession every 3-6 hours from hatching until death.…”