A simple approach to prepare fluorescent molecularly imprinted nanoparticles

Abstract

Fluorescent molecularly imprinted polymer (FMIP) gains great attention in many fields due to their low cost, good biocompatibility and low toxicity. Here, a high-performance FMIP was prepared based on the autocatalytic silica sol–gel reaction.

Customizable molecular recognition: advancements in design, synthesis, and application of molecularly imprinted polymers

“…Additionally, Wang et al focused on creating a new catalyst-free approach to the synthesis of fluorescent MIP (FMIP) nanoparticles using naproxen as a template molecule. 111…”

“…Additionally, Wang et al focused on creating a new catalystfree approach to the synthesis of fluorescent MIP (FMIP) nanoparticles using naproxen as a template molecule. 111 In this method, APTES was coupled with FITC prior to polymerization as a fluorescent silane monomer (FITC-APTES). Varying amounts of FITC-APTES were used to synthesize FMIPs that would allow an observable distribution of FITC fluorophores.…”

Review on molecularly imprinted polymers with a focus on their application to the analysis of protein biomarkers

“…Recently, carbon dots (CDs) have been used as an eco-friendlier, less toxic, and more biocompatible option for fluorescent MIPs [5,10,46,[98][99][100]. In addition, silica NPs can also be loaded with fluorescent dyes and incorporated in the core of MIPs [101]. A search on the MIP database (http://www.mipdatabase.com) in May 2021 for fluorescent MIPs gave about 230 hits, which points out to the extensive use of fluorescent MIPs in recent research articles, especially in the field of sensors and biosensors [102].…”

Three-Dimensional Ordered Macroporous Magnetic Inverse Photonic Crystal Microsphere-Based Molecularly Imprinted Polymer for Selective Capture of Aflatoxin B1

“…MIPs prepared by traditional bulk polymerization method have shortages of irregular particle morphology, deeply embedded templates, and slow mass transfer speed, − which seriously affect their specific recognition capability to the target molecules. Surface molecular imprinting technology supplies a way to overcome above shortages by growing a thin MIP layer onto a suitable supporting carrier with regular structures, e.g., silica spheres. , As the imprinted layers are thin, the templates can be easily removed, and the remaining imprinted cavities is close to the surface which is beneficial to improve the mass transfer efficiency. ,, On the basis of the surface molecular imprinting technology, various MIPs targeting AFB 1 were developed based on different materials: creation of an MIP layer on mesoporous silicas and carbon dots for selective extraction of AFB 1 in food samples which was further analyzed by high-performance liquid chromatography (HPLC), giving a limit of detection (LOD) of 0.118 ng/mL; coating MIP onto the gold chip to make surface plasmon resonance (SPR) sensor with a low LOD of 1.04 pg/mL; grafting MIP recognition sites onto quantum dots , and metal–organic frameworks , to prepare optical sensors with LODs in the level of ng/mL. In general, a dummy template imprinting strategy was mostly employed, e.g., using 5,7-dimethoxycoumarin (DMC) as the template instead of AFB 1 itself, due to pure AFB 1 being expensive and toxic.…”