A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots

Abstract

Publisher rights This is the author's version of a work that was accepted for publication in Journal of Chromatography B. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B, [VOL 923-924, (2013)] General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The authors wish also to acknowledge the Ministry of Higher Education, Malaysia for the studentship provided. The funder of the current study had no involvement in: (1) design and conduct of the study; (2) collection, management, analysis, and interpretation of the data;and (3) preparation, review, or approval of the manuscript. Conflict of Interest:The authors have no conflicts of interest relevant to this article to disclose.Page 2 Chromatographic separation of the AEDs was achieved using Waters XBridge™ C18 column with a gradient system. The developed method was linear over the concentration ranges studied with r ≥0.995 for all compounds. The lower limits of quantification (LLOQs) were 2, 1, 2, 0.5 and 1 μg/mL for LVT, LTG, PHB, CBZE and CBZ, respectively. Accuracy (%RE) and precision (%CV) values for within and between day were <20% at the LLOQs and <15% at all other concentrations tested. This method was successfully applied to the analysis of the AEDs in DBS samples taken from children with epilepsy for the assessment of their adherence to prescribed treatments. Page 3 of 35A c c e p t e d M a n u s c r i p t 4 Highlights: We report a simple method for the analysis of four antiepileptic drugs in DBS samples  The method was applied to DBS samples collected from children with epilepsy  Such technique has potential in assessing adherence to AEDs using home sampling

Therapeutic drug monitoring of carbamazepine and its metabolite in children from dried blood spots using liquid chromatography and tandem mass spectrometry

“…Only one DBS based method for the quantification of CBZ by using HPLC/UV has been reported [19]. However, UV, although useful, are poorly selective and require a complete chromatographic separation of target drugs from the matrix and other drugs, and consequently a relatively long analysis time.…”

“…A relevant number of methods have been published for the quantitation of CBZ alone or in combination with its metabolite in human matrices, like DBS, plasma, serum, urine, brain homogenates, and brain microdialysates [19][20][21][22][23]. These methods imply immunoassays, capillary electrophoresis (CE), micellar electrokinetic capillary chromatography (MEKC), high performance liquid chromatography (HPLC).…”

Distinguishing Intracerebral Hemorrhage from Acute Cerebral Infarction through Metabolomics

“…To satisfy the requirement of rapid stroke diagnosis in the clinical setting, direct infusion MS technology was used in this study. The dried blood spot (DBS ) sample technique has been applied in various fields, such as targeted blood metabolite analysis and newborn screening 13,14 . The profilings of amino acid and carnitine/acylcarnitine in DBS samples have been devoted to disease research 15 .…”

Novel contribution in online sample cleanup for quantitative determination of Levetiracetam in human plasma by LC-Tandem mass spectrometry

“…Most of these methods are utilized different chromatographic techniques such as Gas chromatography (GC) with nitrogen-phosphorus detection, High performance liquid chromatography (HPLC) and GC-MS [7,8]. There are numerous HPLC techniques [9][10][11][12][13][14][15][16], however there are few reported methods for the analysis of LEV using LC-MS/MS [17][18][19][20]. Two of which utilize a tedious solid phase extraction (SPE) procedure of sample extraction [17,19].…”