A simple chemo-enzymatic synthesis of alkyl-acyl (plasmanyl) phospholipids

Solitary and Synergistic Effects of Different Hydrophilic and Hydrophobic Phospholipid Moieties on Rat Behaviors

“…Plasmanylcholines were synthesized according to a previous report [ 14 ]. Briefly, Lyso-PAF C-16 (0.1 mmol), DHA (0.2 mmol), EDC-HCl (0.24 mmol), and DMAP (0.01 mmol) were dissolved in dry dichloromethane (2 mL) and stirred for 3 days at 37 °C.…”

“…Phospholipase-D-mediated head group exchange was carried out in a solvent–water biphasic reaction system [ 14 ]. For the synthesis of PE(O-16:0/22:6), a reaction mixture consisting of 20 mg of PC(O-16:0/22:6) dissolved in 2 mL of chloroform, 1.8 mL of 3 M aqueous ethanolamine solution (pH 5.6, adjusted with acetic acid) and 200 μL of 0.16 mg/mL wild-type phospholipase D from Streptomyces antibioticus was incubated at 37 °C for 24 h with vigorous mixing.…”

“…Plasmanylcholines were synthesized according to a previous report [14]. Briefly, Lyso-PAF C-16 (0.1 mmol), DHA (0.2 mmol), EDC-HCl (0.24 mmol), and DMAP (0.01 mmol) were dissolved in dry dichloromethane (2 mL) and stirred for 3 days at 37 • C. Next, 4 mL of 5% KHSO 4 , 4 mL chloroform, and 2 mL methanol were added to quench the reaction before the mixture was centrifuged briefly for phase separation.…”