A Simple Colorimetric Au‐on‐Au Tip Sensor with a New Functional Nucleic Acid Probe for Food‐borne Pathogen <i>Salmonella typhimurium</i>

Li, Jiuxing; Khan, Shadman; Gu, Jimmy; Filipe, Carlos D. M.; Didar, Tohid F.; Li, Yingfu

doi:10.1002/ange.202300828

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…Recognizing that many food monitoring systems employ oligonucleotide probes, we immobilized fluorophore-labelled, single-stranded DNA oligonucleotides onto transparent polyethylene food wraps, which were then imaged with target food products 23 , 40 – 42 . To this end, DNA oligonucleotides labelled with Pacific Blue, FAM, Cy3, and Cy5 fluorophores—compatible with DAPI, FITC, TRITC, and Cy5 channels, respectively, were deposited onto the polymer substrate via microcontact printing.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive fluorescence profiles of contamination-prone foods applied to the design of microcontact-printed in situ functional oligonucleotide sensors

Khan,

Shakeri,

Monteiro

et al. 2024

Sci Rep

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With both foodborne illness and food spoilage detrimentally impacting human health and the economy, there is growing interest in the development of in situ sensors that offer real-time monitoring of food quality within enclosed food packages. While oligonucleotide-based fluorescent sensors have illustrated significant promise, the development of such on-food sensors requires consideration towards sensing-relevant fluorescence properties of target food products—information that has not yet been reported. To address this need, comprehensive fluorescence profiles for various contamination-prone food products are established in this study across several wavelengths and timepoints. The intensity of these food backgrounds is further contextualized to biomolecule-mediated sensing using overlaid fluorescent oligonucleotide arrays, which offer perspective towards the viability of distinct wavelengths and fluorophores for in situ food monitoring. Results show that biosensing in the Cyanine3 range is optimal for all tested foods, with the Cyanine5 range offering comparable performance with meat products specifically. Moreover, recognizing that mass fabrication of on-food sensors requires rapid and simple deposition of sensing agents onto packaging substrates, RNA-cleaving fluorescent nucleic acid probes are successfully deposited via microcontact printing for the first time. Direct incorporation onto food packaging yields cost-effective sensors with performance comparable to ones produced using conventional deposition strategies.

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Section: Resultsmentioning

confidence: 99%

Comprehensive fluorescence profiles of contamination-prone foods applied to the design of microcontact-printed in situ functional oligonucleotide sensors

Khan,

Shakeri,

Monteiro

et al. 2024

Sci Rep

Self Cite

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Advancing In Situ Food Monitoring through a Smart Lab‐in‐a‐Package System Demonstrated by the Detection of Salmonella in Whole Chicken

et al. 2023

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With food production shifting away from traditional farm‐to‐table approaches to efficient multistep supply chains, the incidence of food contamination has increased. Consequently, pathogen testing via inefficient culture‐based methods has increased, despite its lack of real‐time capabilities and need for centralized facilities. While in situ pathogen detection would address these limitations and enable individual product monitoring, accurate detection within unprocessed, packaged food products without user manipulation has proven elusive. Herein, “Lab‐in‐a‐Package” is presented, a platform capable of sampling, concentrating, and detecting target pathogens within closed food packaging, without intervention. This system consists of a newly designed packaging tray and reagent‐infused membrane that can be paired universally with diverse pathogen sensors. The inclined food packaging tray maximizes fluid localization onto the sensing interface, while the membrane acts as a reagent‐immobilizing matrix and an antifouling barrier for the sensor. The platform is substantiated using a newly discovered Salmonella‐responsive nucleic acid probe, which enables hands‐free detection of 103 colony forming units (CFU) g−1 target pathogen in a packaged whole chicken. The platform remains effective when contamination is introduced with toolsand surfaces, ensuring widespread efficacy. Its real‐world use for in situ detection is simulated using a handheld fluorescence scanner with smartphone connectivity.

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Machine learning-enabled colorimetric sensors for foodborne pathogen detection

Holliday,

Zhang

2024

Advances in Food and Nutrition Research

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A Simple Colorimetric Au‐on‐Au Tip Sensor with a New Functional Nucleic Acid Probe for Food‐borne Pathogen Salmonella typhimurium

Cited by 4 publications

References 42 publications

Comprehensive fluorescence profiles of contamination-prone foods applied to the design of microcontact-printed in situ functional oligonucleotide sensors

Comprehensive fluorescence profiles of contamination-prone foods applied to the design of microcontact-printed in situ functional oligonucleotide sensors

Advancing In Situ Food Monitoring through a Smart Lab‐in‐a‐Package System Demonstrated by the Detection of Salmonella in Whole Chicken

Machine learning-enabled colorimetric sensors for foodborne pathogen detection

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