A simple colorimetric determination of the free amino groups in water soluble chitin derivatives using genipin

Ramos-Ponce, Luz M; Vega, M.; Sandoval, Georgina; Colunga-Urbina, Edith; Balagurusamy, Nagamani; Rodríguez-González, Francisco; Contreras-Esquivel, Juan Carlos

doi:10.1007/s10068-010-0096-9

Cited by 14 publications

(4 citation statements)

References 32 publications

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“…Samples of 5 mL were taken from extracts to determine the cross-linked genipin [4]. A calibration curve ( Fig.…”

Section: Chemical and Statistical Analysismentioning

confidence: 99%

“…It is also known as jagua, juito, huito and genipa (Spanish areas in Latin America), genipap and genipa (English), bois de fer (French) and genipapo (Portuguese) [2]. G. americana and Gardenia jasminoides Ellis fruits contain genipin, an iridoid cross-linking compound [3,4] that is able to react spontaneously with primary amino acids groups, peptides or proteins to form dark blue pigments. It is soluble in water, alcohol and propylene-glycol and it presents stability in the pH range from 4 to 9.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, genipin has been included in analytical purposes, such as aminoacid quantification [14], where studies have been carried out to elucidate the mechanism and kinetics of the cross-linking reaction between biopolymers containing primary amine groups and genipin [15]. Ramos-Ponce et al (2010) have demonstrated that D-glucosamine is an amino sugar that can be cross-linked with genipin to determine the amount of genipin that is present in a sample [4]. Fig.…”

Section: Introductionmentioning

confidence: 99%

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Environmental friendly cold-mechanical/sonic enzymatic assisted extraction of genipin from genipap (Genipa americana)

Ramos‐de‐la‐Peña

Renard

Wicker

et al. 2014

Ultrasonics Sonochemistry

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An efficient cold-mechanical/sonic-assisted extraction technique was developed for extraction of genipin from genipap (Genipa americana) peel. Ultrasound assisted extraction (285 W, 24 kHz) was performed at 5, 10 and 15 °C for 5, 10 and 15 min. After cold-extraction, genipin was separated from pectin and proteins by aid of fungal pectinesterase. The maximum yield of non-cross-linked genipin was 7.85±0.33 mg/g, at 10 °C for 15 min by means of ultrasound extraction. The protein amount in extracts decreased in all samples. If mechanical process is combined with ultrasound assisted extraction the yield is increased by 8 times after the pectinesterase-assisted polyelectrolyte complex formation between pectic polysaccharides and proteins, avoiding the typical cross-linking of genipin. This novel process is viable to obtain non-cross-linked genipin, to be used as a natural colorant and cross-linker in the food and biotechnological industries.

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“…Samples of 5 mL were taken from extracts to determine the cross-linked genipin [4]. A calibration curve ( Fig.…”

Section: Chemical and Statistical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Environmental friendly cold-mechanical/sonic enzymatic assisted extraction of genipin from genipap (Genipa americana)

Ramos‐de‐la‐Peña

Renard

Wicker

et al. 2014

Ultrasonics Sonochemistry

Self Cite

View full text Add to dashboard Cite

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“…The available methods for recovery of genipin and geniposide were described, as well as the methods for genipin and geniposide identification and quantification based on instrumental analyses. Analytical methods for genipin were implemented in view of effective recovery protocols [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ].…”

Section: Introduction and Scopementioning

confidence: 99%

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

et al. 2015

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The present review article intends to direct attention to the technological advances made since 2009 in the area of genipin-crosslinked chitosan (GEN-chitosan) hydrogels. After a concise introduction on the well recognized characteristics of medical grade chitosan and food grade genipin, the properties of GEN-chitosan obtained with a safe, spontaneous and irreversible chemical reaction, and the quality assessment of the gels are reviewed. The antibacterial activity of GEN-chitosan has been well assessed in the treatment of gastric infections supported by Helicobacter pylori. Therapies based on chitosan alginate crosslinked with genipin include stem cell transplantation, and development of contraction free biomaterials suitable for cartilage engineering. Collagen, gelatin and other proteins have been associated to said hydrogels in view of the regeneration of the cartilage. Viability and proliferation of fibroblasts were impressively enhanced upon addition of poly-l-lysine. The modulation of the osteocytes has been achieved in various ways by applying advanced technologies such as 3D-plotting and electrospinning of biomimetic scaffolds, with optional addition of nano hydroxyapatite to the formulations. A wealth of biotechnological advances and know-how has permitted reaching outstanding results in crucial areas such as cranio-facial surgery, orthopedics and dentistry. It is mandatory to use scaffolds fully characterized in terms of porosity, pore size, swelling, wettability, compressive strength, and degree of acetylation, if the osteogenic differentiation of human mesenchymal stem cells is sought: in fact, the novel characteristics imparted by GEN-chitosan must be simultaneously of physico-chemical and cytological nature. Owing to their high standard, the scientific publications dated 2010–2015 have met the expectations of an interdisciplinary audience.

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A comprehensive review on genipin: an efficient natural cross-linker for biopolymers

Wahba

2024

Polym. Bull.

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Cross-linkers are pivotal to meliorate the attributes of the biopolymers, which are exploited in the biomedical industries, and also those intended as packaging supplies. Genipin (GN) is an efficacious cross-linker. Moreover, being naturally procured, biodegradable and biocompatible makes it an auspicious candidate for the biomedical and food industries. Accordingly, we attempted to provide a comprehensive review on GN as an efficient cross-linker for biopolymers. Initially, we presented the chief botanical sources of GN. The GN extraction strategies, which adopted safe solvents, were then discussed while highlighting their realized yields. The proposed GN structures, its possible modes of action, and the factors affecting its interactions, such as pH, temperature, and GN concentration were also reviewed. Afterward, the GN applications that mainly involved cross-linking biopolymers and biopolymers containing materials were discussed. These included tissue engineering, wound dressings, drug delivery, and packaging applications. GN capability to activate biopolymers, such as chitosan and gelatin, into covalently reactive enzyme immobilizers was also discussed. Moreover, other important GN applications, such as exploiting it as a colorant for foods and textiles and incorporating it in altered biosensors, were discussed.

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A simple colorimetric determination of the free amino groups in water soluble chitin derivatives using genipin

Cited by 14 publications

References 32 publications

Environmental friendly cold-mechanical/sonic enzymatic assisted extraction of genipin from genipap (Genipa americana)

Environmental friendly cold-mechanical/sonic enzymatic assisted extraction of genipin from genipap (Genipa americana)

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

A comprehensive review on genipin: an efficient natural cross-linker for biopolymers

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