A simple colorimetric experiment using mammalian cell culture to study metabolism

Sharma, Kanika; Fizet, Kiel J.; Montgomery, Kathlyene R.; Smeltzer, Nathan A.; Sikorski, Madison H.; Brown, Kennedy G.; Beyke, Brandon J.; Burkhart, Ryan C.; Lynn, Abigail N.; Grandinetti, Giovanna

doi:10.1002/bmb.21457

Cited by 3 publications

References 9 publications

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2-Aminopyridine Chemicals Kill Pc-3 Prostate Cancer Cells Via Activation of Ampk and Indirect Inhibition of the Mtor Pathway

Valdes,

Botelho,

Lima

et al. 2024

Preprint

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2-Aminopyridine Chemicals Kill Pc-3 Prostate Cancer Cells Via Activation of Ampk and Indirect Inhibition of the Mtor Pathway

Valdes,

Botelho,

Lima

et al. 2024

Preprint

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Complementary Metal Oxide Semiconductor-Based Optical Detection System for Fluidic Cellular Medium pH Quantification

Santos,

Ferreira,

Sousa

et al. 2024

Photonics

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Accurate pH sensing is essential for the effective physiological control of the culture media in organ-on-a-chip (OoC) systems. This work proposes a miniaturized optical detection system, based on optical transmittance, for pH level quantification of the EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ culture medium. Firstly, using a commercial spectrophotometric setup, a set of wavelengths (500, 560, and 600 nm) was selected, as these wavelengths assure distinctive slope variations for the different pH levels. Then, a current-to-frequency converter, based on a low-power Schmitt trigger model with a voltage enhancer, was proposed as the readout electronics and simulated in Cadence Tools using UMC L180 MM/RF technology. A resolution of 0.002 nA was achieved in the linear range of 30 pA to 3800 nA. A miniaturized system composed of a CMOS n-well/p-substrate photodiode and a polydimethilsiloxane (PDMS) microchannel for the culture media substrate was experimentally tested. For a pH range from 6.6 to 6.2, the results clearly demonstrate a magnitude shift of the slope signal, which becomes negative in basic media and positive in acidic media. Additionally, in the 500–560 nm spectral range, the amplitude of the slopes increases for both basic and acidic culture media. In the 560–600 nm range, the slope decreases progressively as the pH of the medium lowers. This miniaturized system was able to quantify the pH of the culture medium, showing potential to be integrated into an organ-on-a-chip device.

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Evaluation of the use of virtual simulators for training in problem-solving skills in university students

Cajamarca Carrazco,

Hidalgo Cajo,

Jhon Alexander Ponce Alencastro

et al. 2024

Salud, Ciencia y Tecnología

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The objective of this article is to evaluate the effectiveness of virtual simulators in training problem-solving skills in university students, a crucial aspect for their professional performance. To carry out this evaluation, a documentary review of various surveys and case studies was carried out. Academic articles, technical reports, and student and faculty survey data were analyzed to obtain a comprehensive view of the use of virtual simulators in higher education.Research results indicate that virtual simulators not only improve students' theoretical understanding, but also allow them to apply their knowledge in controlled and safe environments, replicating real-world situations. These simulators provide an environment where students can make mistakes and learn from them without real consequences, strengthening their ability to address complex problems and make informed decisions. The case studies reviewed show specific benefits in various academic disciplines, highlighting a positive impact on academic performance and career preparation.However, challenges and limitations were identified, such as the cost of implementing the simulators and the need for adequate training for instructors. Evaluating the use of virtual simulators for training problem-solving skills in university students is a crucial area of study in contemporary education. The research reviewed highlights the growing importance of these educational technologies in promoting more interactive and practical learning, facilitating the development of skills essential for success.

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A simple colorimetric experiment using mammalian cell culture to study metabolism

Cited by 3 publications

References 9 publications

2-Aminopyridine Chemicals Kill Pc-3 Prostate Cancer Cells Via Activation of Ampk and Indirect Inhibition of the Mtor Pathway

2-Aminopyridine Chemicals Kill Pc-3 Prostate Cancer Cells Via Activation of Ampk and Indirect Inhibition of the Mtor Pathway

Complementary Metal Oxide Semiconductor-Based Optical Detection System for Fluidic Cellular Medium pH Quantification

Evaluation of the use of virtual simulators for training in problem-solving skills in university students

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