A simple concentration method for the detection of parasitic ova and cysts in faeces.

Thompson, R. G.

doi:10.1136/jcp.25.6.546

Cited by 5 publications

(3 citation statements)

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“…Blood count included haemoglobin and peripheral white blood count and differential formula; determination of semi quantitative CRP was performed using the commercial kit Humatex ® CRP(Human Gessellshaff, Wiesbaden, Germany); sodium, potassium and calcium in plasma using Photometry using Sherwood ® Model 410 Classic Flame Photometer Range (Sherwood Scientific Limited, Cambridge, UK); latex test for rotavirus using Rida ® Quick Rotavirus/Adenovirus Combi (R-Biopharm AG, Darmstadt, Germany) and parasitological assessment by PAF technique by Lugol method [ 17 ]. Stool culture was performed for enteropathogenic, toxigenic, enteroadherent, enteroaggregative, invasive and enterohemorrhagic E coli , Salmonella typhi and Shigella sp , applying routine procedures (Agar base, MacConkey Agar, SS agar and Tetrahionate Broth Base.…”

Section: Methodsmentioning

confidence: 99%

Probiotics in the treatment of acute rotavirus diarrhoea. A randomized, double-blind, controlled trial using two different probiotic preparations in Bolivian children

Medina²,

et al. 2010

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BackgroundEvidence suggests that probiotics reduce rotavirus diarrhoea duration. Although there are several probiotic strains potentially useful, daily practice is often limited by the type and number of products locally available. In general, information about combined products is scarce. In this study we compare the effect of two probiotic products in the treatment of diarrhoea in children less than 2 years of age.MethodsA Randomized double-blind controlled clinical trial in children hospitalized for acute rotavirus diarrhoea, in the Paediatric Centre Albina Patino, Cochabamba, Bolivia.Participants were children aged 1 - 23 months, who were randomly assigned to receive one of three treatments: Oral rehydration therapy plus placebo; Oral rehydration solution plus Saccharomyces boulardii; or Oral rehydration solution plus a compound containing Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium longum and Saccharomyces boulardii. Sample size was 20 per group and the outcomes were duration of diarrhoea, of fever, of vomiting and of hospitalization.Results64 cases finished the protocol. On admission, patients' characteristics were similar. Median duration of diarrhoea (p = 0.04) in children who received the single species product (58 hours) was shorter than in controls (84.5 hrs). Comparing children that received the single probiotic product and controls showed shorter duration of fever (18 vs 67 hrs) (p = 0.0042) and the mixed probiotic of vomiting (0 vs 42.5 hrs) (p = 0.041). There was no effect on duration of hospitalization (p = 0.31). When experimental groups were merged, statistical significance of changes increased (total duration of diarrhoea, fever and vomiting P = 0.025, P = 0.025 and P = 0.014, respectively).ConclusionsBoth products decreased the duration of diarrhoea compared to oral rehydration solution alone. This decrease was significant only for the single species product which also decreased the duration of fever. With the multiple species product there was no vomiting subsequent to the initiation of treatment. The quantity of probiotic bacteria needed for optimum treatment of gastroenteritis remains to be determined, particularly when multiple species are included in the product.Trial registration: ClinicalTrials.gov ID: NCT00981877Link: https://register.clinicaltrials.gov/prs/app/action/SelectProtocol/sid/S0002653/selectaction/View/ts/2/uid/U0000N04Trial RegistrationClinical trials NCT ID: NCT00981877

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Section: Methodsmentioning

confidence: 99%

Probiotics in the treatment of acute rotavirus diarrhoea. A randomized, double-blind, controlled trial using two different probiotic preparations in Bolivian children

Medina²,

et al. 2010

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“…For the detection of rotavirus in faeces the Rida Quick Rotavirus kit was used (R-Biopharm, Darmstadt, Germany). Parasitological assessment by PAF technique by Lugol method [13]. Stool cultures were performed for enteropathogenic, toxigenic, enteroadhaerent, enteroaggregative, invasive and enterohaemorrhagic E. coli and for Salmonella typhi and Shigella spp using routine procedures (Agar Base, McConkey, SS Agar and Tetrathionate Broth Base (Bacto Difco, Kansas, USA).…”

Section: Methodsmentioning

confidence: 99%

Use of Probiotic Yogurt in the Management of Acute Diarrhoea in Children. Randomized, Double-Blind, Controlled Study

Grandy¹,

Jose²,

Soria³

et al. 2014

OJPed

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Background: In Bolivia there are few probiotic preparations available in the market, practically without alternatives to the use of freeze-dried products. An alternative used as a good alternative in other parts of the world is yogurt with a probiotic strain added. In this study we report the use of a locally prepared yogurt with a lyophilized probiotic added. Methods: This is randomized, double-blind and controlled clinical trial in children hospitalized with acute diarrhoea carried out at the Paediatric Centre Albina Patiño in Cochabamba, Bolivia with children 10 to 35 months participating. Children were randomly assigned to receive an oral rehydration solution and Saccharomyces boulardii (positive control) or the same solution plus yogurt preparation containing Lactobacillus rhamnosus (yogurt group). The primary outcome was the duration of diarrhoea. Secondary outcomes were the duration of fever, vomiting and hospitalization. Results: Of the 74 children incorporated 42 completed the protocol; baseline characteristics were similar in both groups. The median duration of diarrhoea was similar in children who received yogurt (71 hours) and controls (79 hours) (p = 0.3). The mean duration of fever was also similar in both groups: S. boulardii (24 hours) or yogurt (11 hours) (p = 1.02), as was the duration of vomiting: 17 hours vs. 32 hours in the control group (p = 0.37). A significant difference in the numbers of daily bowel movements was observed in the third day, the probiotic group was lower (p =< 0.01). There was no effect on the duration of hospitalization (p = 0.26). Conclusions: The use of a yogurt containing L. rhamnosus has an effect comparable to S. boulardii. Introduction of yoghurt with a probiotic in the diet of patients hospitalized with acute diarrhoea may help reduce its duration, regardless of its aetiology.

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“…The best of these stains is that of Thompson (1972) which also enhances somewhat the structure of cysts: add a drop of a mixture in equal parts of 10 % w/v aqueous nigrosin and 1 % w/v aqueous alcian blue.…”

Section: Examination Of Faecesmentioning

confidence: 99%

The laboratory diagnosis of tropical diseases with special reference to Britain: a review.

Ridley¹

1974

J Clin Pathol

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A simple concentration method for the detection of parasitic ova and cysts in faeces.

Cited by 5 publications

References 1 publication

Probiotics in the treatment of acute rotavirus diarrhoea. A randomized, double-blind, controlled trial using two different probiotic preparations in Bolivian children

Probiotics in the treatment of acute rotavirus diarrhoea. A randomized, double-blind, controlled trial using two different probiotic preparations in Bolivian children

Use of Probiotic Yogurt in the Management of Acute Diarrhoea in Children. Randomized, Double-Blind, Controlled Study

The laboratory diagnosis of tropical diseases with special reference to Britain: a review.

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