A simple, cost efficient fiber amplifier wavelength extension unit for broadly tunable, femtosecond pulse Ti-sapphire lasers for CARS microscopy

Kolonics, Attila; Csáti, Dániel; Antal, P.; Szipöcs, R.

doi:10.1364/biomed.2012.bsu3a.28

Cited by 3 publications

(5 citation statements)

References 5 publications

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“…However, after a relatively simple, cost efficient modification/upgrade of the 2PAF laser and microscope setups, one can make his system capable for CARS microscopy as well. In this section we briefly introduce our relatively simple, cost efficient, fiber laser based CARS extension unit, which can be adopted for most of the femtosecond pulse, tunable Ti:sapphire lasers used for 2PAF imaging (Kolonics et al, ) (Fig. ).…”

Section: Methodsmentioning

confidence: 99%

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In vivosecond-harmonic generation andex vivocoherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit

Haluszka

Lőrincz

Molnár

et al. 2015

Microsc. Res. Tech.

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Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work we used two different techniques that require no labeling: second harmonic generation (SHG) for collagen detection and coherent anti-Stokes Raman scattering (CARS) to assess lipid distribution in genetically obese murine skin. Obesity is one of the most serious public health problems due to its high and increasing prevalence and the associated risk of type 2 diabetes and cardiovascular diseases. Other than these diseases, nearly half of patients with diabetes mellitus suffer from dermatological complications such as delayed wound healing, foot ulcers and several other skin changes. In our experiment we investigated and followed the effects of obesity on dermal collagen alterations and adipocyte enlargement using a technique not reported in the literature so far. Our results indicate that the in vivo SHG and ex vivo CARS imaging technique might be an important tool for diagnosis of diabetes-related skin disorders in the near future.

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Section: Methodsmentioning

confidence: 99%

“…In this section we briefly introduce our relatively simple, cost efficient, fiber laser based CARS extension unit, which can be adopted for most of the femtosecond pulse, tunable Ti:sapphire lasers used for 2PAF imaging (Kolonics et al, 2012) (Fig. 1).…”

Section: Imaging Setupmentioning

confidence: 99%

In vivosecond-harmonic generation andex vivocoherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit

Haluszka

Lőrincz

Molnár

et al. 2015

Microsc. Res. Tech.

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show abstract

“…This latter wavelength corresponds to the CH 2 symmetric stretching vibration resonance of saturated fatty acids in our setup. Further details on the optical setup can be found in Ref [23]. 3D images of the skin samples were obtained by computer controlled accurate positioning of the objective along the z-axis ("z-stack images"), which enabled us imaging the samples in different tissue depths.…”

Section: Imaging Setupmentioning

confidence: 99%

Diet-induced obesity skin changes monitored by in vivo SHG and ex vivo CARS microscopy

Haluszka¹,

Lőrincz²,

Kiss³

et al. 2016

Biomed. Opt. Express

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Obesity related metabolic syndrome and type 2 diabetes have severe consequences on our skin. Latest developments in nonlinear microscopy allow the use of noninvasive, label free imaging methods, such as second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS), for early diagnosis of metabolic syndrome-related skin complications by 3D imaging of the skin and the connective tissue. Our aim was to study effects of various types of diet-induced obesity in mice using these methods. We examined mice on different diets for 32 weeks. The collagen morphology was evaluated four times by SHG microscopy, and adipocytes were examined once at the end of experiment by CARS method. A strong correlation was found between the body weight and the adipocyte size, while we found that the SHG intensity of dermal collagen reduces considerably with increasing body weight. Obese mice on high-fat diet showed worse results than those on high-fat - high-fructose diet. Animals on high-fructose diet did not gain more weight than those on ordinary diet despite of the increased calorie intake, but their collagen damage was nonetheless significant. Obesity and high sugar intake damages the skin, mainly the dermal connective tissue and subcutaneous adipose tissue, which efficiently can be monitored by SHG and CARS microscopy.

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“…The question arises: how well the distorted modes of dispersive fibers can be applied, for example, for two photon microscopy, where focusability of the fiber delivered ultrashort pulses is of primary importance [4]. In order to investigate this question, we calculate the intensity square of the mode field distribution in the Fourier space, which can be related to the effectiveness of the two-photon absorption or second harmonic generation in the focal plane of a focusing element, such as a microscope objective.…”

Section: B Focusability Of the Distorted Modesmentioning

confidence: 99%

“…Recently, we have shown [3] that reversed slope (S < 0) or flat (S = 0), anomalous (D > 0), or zero (D = 0) dispersion functions can be obtained in a wide wavelength range by introducing resonant structures in the fiber cladding in hollow-core, air-silica photonic bandgap (PBG) fibers or in solid core Bragg fibers with step-index profile. Such optical fibers could be well suited for distortion free delivery or high quality pulse compression of broadly tunable or broadband femtosecond laser pulses in all fiber laser systems, such as femtosecond pulse, all-fiber laser oscillators [2], and amplifiers [4] being suitable for nonlinear microscopy. During the last few years, novel, all-fiber, multimodal (TPF + SHG + CARS) microscope systems have been presented [5]- [7] that can be combined with endoscopy [7], which would greatly increase the utility of nonlinear microscopy for pre-clinical applications and tissue imaging [8].…”

Section: Introductionmentioning

confidence: 99%

Stored Energy, Transmission Group Delay and Mode Field Distortion in Optical Fibers

Várallyay

Szipöcs

2014

IEEE J. Select. Topics Quantum Electron.

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The relationship between transmission group delay and stored energy in optical fibers is discussed. We show by numerical computations that the group delay of an optical pulse of finite bandwidth transmitted through a piece of a low loss optical fiber of unit length is proportional to the energy stored by the standing wave electromagnetic field. The stored energy-group delay ratio typically approaches unity as the confinement loss converges to zero. In case of a dispersion tailored Bragg fiber, we found that the stored energy-group delay ratio decreased while the confinement loss increased compared to those of the standard quarterwave Bragg fiber configuration. Furthermore, a rapid variation in the group delay versus wavelength function due to mode-crossing events (in hollow core photonic bandgap fibers for instance) or resonances originating from slightly coupled cavities, surface or leaking modes in index guiding, photonic bandgap, or photonic crystal fibers always results in a rapid change in the mode-field distribution, which seriously affects splicing losses and focusability of the transmitted laser beam. All of these factors must be taken into consideration during the design of dispersion tailored fibers for different applications.

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A simple, cost efficient fiber amplifier wavelength extension unit for broadly tunable, femtosecond pulse Ti-sapphire lasers for CARS microscopy

Cited by 3 publications

References 5 publications

In vivosecond-harmonic generation andex vivocoherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit

In vivosecond-harmonic generation andex vivocoherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit

Diet-induced obesity skin changes monitored by in vivo SHG and ex vivo CARS microscopy

Stored Energy, Transmission Group Delay and Mode Field Distortion in Optical Fibers

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