A simple device for controlled feeding of pasty materials used as fermentation substrates

Abstract

A simple device is described for controlled feeding of pasty materials in laboratory scale fed-batch cultures. The device was tested with apple pomace as raw material for production of microbial biomass, using the level of dissolved oxygen as controlled parameter. Results and discussionThe control unit is depicted in Fig. 2. Any parameter associated with a metabolic activity such as pO2, pCO2, pH, carbon and nitrogen source level, etc.

Microbial biomass product from apple pomace in batch and fed batch cultures

“…Fed-batch experiments were started in fermentors with medium 3 diluted 1.5 times with water. A feeding device (Hours and Massucco 1985) was charged with NTAP, at pH 1.6, including the salts used in medium 3 but without water, and adjusted to a DO reference value of 25%, with an addition time of 1 sec, a waiting time of 90 sec and using a motor speed of 1000 rpm.…”