A simple device to rapidly prepare whole mounts of murine intestine

Rudling, R.; Hassan, Amber; Kitau, J.; Mandir, Nikki; Goodlad, R A

doi:10.1111/j.1365-2184.2006.00391.x

Cited by 20 publications

(25 citation statements)

References 11 publications

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“…Briefly, the small intestine was divided into three equal sections: proximal, middle, and distal. These sections, as well as the entire colon, were then dissected longitudinally using a recently described cutting guide (30) and fixed in Carnoy's fluid for 2 h and stored in 70% ethanol. For macroscopic polyp counts assessed with a dissecting microscope (at Â20 magnification), the number and size (mean of two largest diameters measured with digital calipers) of polyps in the small and large intestines were determined.…”

Section: Methodsmentioning

confidence: 99%

Dual inhibition of VEGFR and EGFR signaling reduces the incidence and size of intestinal adenomas in ApcMin/+ mice

Alférez

Wilkinson²,

Watkins³

et al. 2008

Molecular Cancer Therapeutics

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Both the epidermal growth factor (EGF) and the vascular endothelial growth factor (VEGF) pathways are associated with intestinal cancer, and therapeutic approaches targeting either EGF receptor (EGFR) or VEGF receptor (VEGFR) signaling have recently been approved for patients with advanced colorectal cancer. The Apc Min/+ mouse is a well-characterized in vivo model of intestinal tumorigenesis, and animals with this genetic mutation develop macroscopically detectable adenomas from f6 weeks of age. Previous work in the Apc Min/+ mouse has shown that therapeutic approaches targeting either VEGFR or EGFR signaling affect predominantly the size or number of adenomas, respectively. In this study, we have assessed the effect of inhibiting both these key pathways simultaneously using ZD6474 (Vandetanib, ZACTIMA), a selective inhibitor of VEGFR and EGFR tyrosine kinases. To assess the effects of ZD6474 on early-and later-stage disease, treatment was initiated in 6-and 10-week-old

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Section: Methodsmentioning

confidence: 99%

Dual inhibition of VEGFR and EGFR signaling reduces the incidence and size of intestinal adenomas in ApcMin/+ mice

Alférez

Wilkinson²,

Watkins³

et al. 2008

Molecular Cancer Therapeutics

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“…We have recently described the construction and use of a device to aid the process (Rudling et al 2006). Samples of tissue can be fixed in appropriate containers or whole mounts prepared.…”

Section: Gut Preparationmentioning

confidence: 99%

“…Whole mounts are very useful if other measures, such as searching for aberrant crypt foci, for polyps or 'micropolyp' formation are intended. We have recently described the construction and use of a device to aid the process (Rudling et al 2006). In brief, stainless steel rods are threaded through the segments of intestine and a cutting guide is used to guide a scalpel and dissect the segments longitudinally.…”

Section: Gut Preparationmentioning

confidence: 99%

To best measure cell proliferation in samples from the intestine

Alférez

Goodlad

2007

Cell Proliferation

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Arrangement of the intestinal cell lining, as it is, into distinct anatomically defined zones where proliferation is confined to the crypts, makes it an ideal tissue to study growth control mechanisms. While many methods have been used to quantify cell proliferation in the gut, several of them have severe limitations and others (although potentially better) have been misused and misinterpreted. Here, correct use and interpretation of labelling studies will be described as will a well established alternative method that provides equivalent results for one-sixth of the effort.

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“… F1 (CC‐C57BL/6 Apc Min+/− ) mice were produced by crossing females of the different CC lines with males of C57BL/6 Apc Min+/− . Genotypes of the F1 for C57BL/6 Apc Min+/− was determined by PCR. F1 (CC‐C57BL/6 Apc Min+/− ) were kept for 5 months on a standard mouse chow diet and water ad libitum. Mice were terminated, and their small intestine and colon were extracted and washed with PBS. The small intestine were divided into three segments (SB1—proximal, SB2—middle, and SB3—distal), and the colon was kept as whole and spread over a 3‐mm paper. The intestines were fixed in 10% NBF overnight and stained with 0.02% methylene blue. The samples were then examined by binuclear. The number and sizes of polyps in each of the four intestinal subregions were recorded as described …”

Section: The Usage Of the CC Mouse Populationmentioning

confidence: 99%

Efficient protocols and methods for high‐throughput utilization of the Collaborative Cross mouse model for dissecting the genetic basis of complex traits

Atamni

Iraqi

2019

Anim Models and Exp Med

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The Collaborative Cross (CC) mouse model is a next‐generation mouse genetic reference population (GRP) designated for a high‐resolution quantitative trait loci (QTL) mapping of complex traits during health and disease. The CC lines were generated from reciprocal crosses of eight divergent mouse founder strains composed of five classical and three wild‐derived strains. Complex traits are defined to be controlled by variations within multiple genes and the gene/environment interactions. In this article, we introduce and present variety of protocols and results of studying the host response to infectious and chronic diseases, including type 2 diabetes and metabolic diseases, body composition, immune response, colorectal cancer, susceptibility to Aspergillus fumigatus, Klebsiella pneumoniae, Pseudomonas aeruginosa, sepsis, and mixed infections of Porphyromonas gingivalis and Fusobacterium nucleatum, which were conducted at our laboratory using the CC mouse population. These traits are observed at multiple levels of the body systems, including metabolism, body weight, immune profile, susceptibility or resistance to the development and progress of infectious or chronic diseases. Herein, we present full protocols and step‐by‐step methods, implemented in our laboratory for the phenotypic and genotypic characterization of the different CC lines, mapping the gene underlying the host response to these infections and chronic diseases. The CC mouse model is a unique and powerful GRP for dissecting the host genetic architectures underlying complex traits, including chronic and infectious diseases.

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A simple device to rapidly prepare whole mounts of murine intestine

Cited by 20 publications

References 11 publications

Dual inhibition of VEGFR and EGFR signaling reduces the incidence and size of intestinal adenomas in ApcMin/+ mice

Dual inhibition of VEGFR and EGFR signaling reduces the incidence and size of intestinal adenomas in ApcMin/+ mice

To best measure cell proliferation in samples from the intestine

Efficient protocols and methods for high‐throughput utilization of the Collaborative Cross mouse model for dissecting the genetic basis of complex traits

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