A simple DNA coprecipitation method for the detection of diatoms in heart blood

Seo, Yasuhisa; Sato, Shingo; Kuroki, Kohji; Kishida, T

doi:10.1016/j.forsciint.2013.07.016

Cited by 11 publications

(6 citation statements)

References 21 publications

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“…In the previous examples proteinase K was added to HRP in equimass quantities. Proteases are usually applied in much smaller quantities than the protein to be digested (10-1000 : 1) 34,35 to minimize self-digestion. We therefore performed a digestion on HRP pretreated with RapiGest™, DTT and IAM using only 10% proteinase K compared to HRP's mass.…”

Section: Resultsmentioning

confidence: 99%

Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

et al. 2017

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Section: Resultsmentioning

confidence: 99%

Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

et al. 2017

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“…Pleural effusion, decreased weight of spleen, middle ears hemorrhage and fluid accumulation in the stomach are other macroscopic findings. Concerning the microscopic findings, hematoxylin and eosin staining used in histological investigations depict intra-alveolar edema and dilatation of the alveolar spaces with secondary compression of the septal capillaries (Seo et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Histopathological Differentiation of Drowning in Freshwater and Saltwater in Rats: Forensic Point of View

et al. 2022

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The postmortem diagnosis of death by drowning is one of the most difficult issues in forensic pathology. We investigated possible evidence differentiating saltwater drowning from freshwater drowning by histopathological changes in brain, heart, lungs, liver, and kidneys tissues. A cross section descriptive study was carried out on eighteen 12-week-old male Wistar rats; they were divided equally into 3 groups. Group 1: control group; Group 2: death by drowning in freshwater; Group 3: death by drowning in saltwater. Immediately after death, all tested organs were removed and fixed for histopathological examination. The brain of freshwater group depicted degenerated neurocytes with dystrophic changes in the form of shrunken cell, pyknotic nuclei and deeply eosinophilic cytoplasm. The heart showed clear evidence of myocyte injuries in saltwater drowning compared to the control and freshwater groups. The kidneys of rats drown in saltwater revealed more glomerular destruction with no differences in tubulo-interstitial changes in comparison with those drown in freshwater. In the lungs, the changes in freshwater were restricted to the alveoli, and the bronchial changes were more distinctive in saltwater. No disturbed liver architecture was seen in both test groups, however hydropic degeneration, congested vessels, and sinusoids were more distinct in saltwater group. In conclusion, diagnostic differentiation between fresh and saltwater drowning was reliable in rats' lungs and heart with minimal differentiation in liver, kidneys, and brain. Further studies of drowning with different staining techniques will help to clarify the potential role of histopathological changes in body organs as indicator of drowning.

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“…The autopsy diagnosis of drowning is based on macroscopic and microscopic findings [2,3]. Externally, the presence of foam at the mouth or nostrils, or both, is important.…”

Section: Discussionmentioning

confidence: 99%

“…The polymerase chain reaction method represents a new perspective for identifying diatoms but its higher sensitivity could be hampered by a higher risk for contamination [4]. Recently, Seo et al [3] suggested a DNA coprecipitation method for the detection of diatoms in heart blood as a simple and safe tool for the diagnosis of drowning.…”

Section: Discussionmentioning

confidence: 99%

“…The autopsy diagnosis of drowning is one of the major problems in forensic medicine, especially when there is a delay in recovering the corpse. The forensic diagnosis of drowning is based on macroscopic and microscopic findings but the pathological proof is often difficult or even impossible to obtain [2,3]. Furthermore, the macroscopic and microscopic findings in the fresh drowned corpse are non-specific and will disappear with putrefaction [4].…”

Section: Introductionmentioning

confidence: 99%

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Role of virtopsy in the post-mortem diagnosis of drowning

Vernuccio

Galfano

et al. 2014

Radiol med

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Purpose Due to admitted limits of autopsy-based studies in the diagnosis of drowning, virtopsy is considered the new imaging horizon in these post-mortem studies. The aim of our study was to evaluate the role of virtopsy performed through computed tomography (CT) in the forensic diagnosis of drowning. Materials and methods We retrospectively examined the CT data of four cadavers recovered from sea water and suspected to have died by drowning. Each patient underwent a full-body post-mortem CT scan, and then a traditional autopsy. Results All the cadavers showed fluid in the airways and patchy ground-glass opacities in the lung. Only one patient had no fluid in the digestive tract; this patient had a left parietal bone fracture with a large gap and other multiple bone fractures (nose, clavicle, first rib and patella). One of the three patients who had fluid in the digestive tract had no fluid in the paranasal sinuses. This latter patient showed cerebral oedema with subarachnoid and intraventricular haemorrhage, multiple bone fractures (orbital floor, ribs, sacrum and acetabular edge) and air in the heart, in the aorta and in bowel loops. ConclusionTo date, there are no autopsy findings pathognomonic of drowning. This study proves that virtopsy is a useful tool in the diagnosis of drowning in that it allows us to understand if the victim was alive or dead when he entered the water and if the cause of death was drowning.

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A simple DNA coprecipitation method for the detection of diatoms in heart blood

Cited by 11 publications

References 21 publications

Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

Histopathological Differentiation of Drowning in Freshwater and Saltwater in Rats: Forensic Point of View

Role of virtopsy in the post-mortem diagnosis of drowning

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