A simple, efficient and economical method for isolating and culturing human umbilical cord blood‑derived mesenchymal stromal cells

Abstract

Mesenchymal stromal cells (MScs) hold broad therapeutic potential in various diseases, however, it is difficult to produce sufficient numbers of MSCs for clinical application, therefore, improved culture systems are required. The present study aimed to develop a novel method for isolating and culturing human umbilical cord blood-derived mesenchymal stromal cells (hucB-MScs). a sequential culture method was developed that uses two types of culture media to optimize the isolation and culture of hucB-MScs. First, dMeM supplemented with mesenchymal stem cell growth supplement was used to improve the colony formation and primary culture success rates of hucB-MScs. Then, after removing the heterogeneous cell population, ordinary dMeM was used from the fourth passage. This method obtained hucB-MScs with high culture efficiency and at a greatly reduced cost. The optimal culture conditions were determined and the hucB-MScs were phenotypically characterized after passaging. Taken together, this simple, efficient and economical method can produce a large number of high-quality hucB-MScs in <1 month, therefore facilitating the future clinical applications of hucB-MScs.

Human adipose-derived mesenchymal stem cells inhibit proliferation and induce apoptosis of human gastric cancer HGC-27 cells

“…Mesenchymal stem cells (MSCs) can be obtained from umbilical cord, amniotic membrane, adipose tissue, and so on (Cho et al 2019;Han et al 2014;Hendrijantini and Hartono 2019;Zhang et al 2019). MSCs are usually used for the treatment of traumatic and degenerative diseases due to its extensive source, self-renewal potential, multi-lineage differentiation, and immunoregulatory ability (Li et al 2016).…”

Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells

“…In the era of stem cell research, mesenchymal stem cells have been one of the key sources of cell-based therapies [51]. Different research groups identified, isolated, and compared the cellular morphology, surface markers, and differentiation abilities of MSCs derived from different sources [2][3][4][5][6][7][8][9][10][11][12][13][14][15]. A lot of work has been performed to date to investigate the possible therapeutic effects of MSCs in different acute kidney injury models [16][17][18][19][20][21][22][23][24].…”

“…MSCs are undifferentiated, self-renewable, non-hematopoietic adult stem cells of mesodermal origin [1]. These multipotent stem cells are broadly distributed in the body and can be relatively easily isolated from various tissues, such as bone marrow, umbilical cord blood, fat, muscle, periosteum, lung, testis, and dermis [2][3][4][5][6][7][8][9][10][11][12][13][14]. MSCs can differentiate into both mesenchymal and non-mesenchymal lineages, including differentiation toward adipocytes, chondrocytes, osteocytes, skeletal muscle cells, renal cells, and smooth muscle cells [9][10][11]15].…”