A simple enzyme-assisted cascade amplification strategy for ultrasensitive and label-free detection of DNA

MOF-mediated dual energy transfer nanoprobe integrated with exonuclease III amplification strategy for highly sensitive detection of DNA

“…31,32 However, the enzyme-free strategies present challenges such as prolonged reaction cycles and non-specific binding. 33 Enzyme-assisted cascade amplification exhibits improved amplification performance and hybridization specificity; 34 however, the application of certain enzymes, such as DNase I and DNAzymes, is restricted by their sequence-dependent requirements and complex designs. 35,36 On the contrary, exonuclease III (Exo III), enables catalyzing the stepwise hydrolysis of mononucleotide from 3′-blunt end of dsDNA including 5′-overhangs or nicks, and benefits from low cost, simple design, and sequence-independent recognition.…”

Photogenerated Hole-Induced Chemical–Chemical Redox Cycling Strategy on a Direct Z-Scheme Bi2S3/Bi2MoO6 Heterostructure Photoelectrode: Toward an Ultrasensitive Photoelectrochemical Immunoassay

“…Besides the development of various photoactive materials to fabricate the photoelectrode, the exploration of ingenious signaling strategies on the photoelectrode is another powerful aspect in the PEC bioanalysis. Currently, some strategies including the DNA amplification strategy, , enzymes-assisted strategy, , and energy transfer , have been proposed for the sensitivity enhancement. Of these, the PEC chemical–chemical (PECCC) redox cycling amplification (RCA) strategy proposed by our group , has exhibited its great potential for signal amplification.…”