A Simple Enzyme Histochemical Method for the Simultaneous Demonstration of Acetylcholinesterase and Monoamine Oxidase in Fixed-Frozen Sections

Dunning, Daniel D.; McHaffie, John G.; Stein, Barry E.

doi:10.1177/002215549704500614

Cited by 2 publications

(2 citation statements)

References 52 publications

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“…Serial sections were cut (50–60 μm) on a freezing microtome, collected in cold PB, and processed immediately. MAO activity was revealed according to Dunning et al (1997). Briefly, sections were rinsed three times for 1 minute each in PB, preincubated for 15 minutes in 50 mM Tris, pH 7.6, incubated for 4–24 hours at 22–25°C in a solution containing 0.07% tyramine‐HCl (Sigma, St. Louis, MO), 0.005% diaminobenzidine‐HCl, 0.05% horseradish peroxidase Type II (Sigma), 0.065% sodium azide, and 0.6% nickel sulfamate (Sigma) in 50 mM Tris, pH 7.6.…”

Section: Methodsmentioning

confidence: 99%

Developmental expression of monoamine oxidases A and B in the central and peripheral nervous systems of the mouse

Vitalis

Fouquet

Alvarez

et al. 2002

J of Comparative Neurology

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Monoamine oxidases A (MAOA) and B (MAOB) are key players in the inactivation pathway of biogenic amines. Their cellular localization has been well established in the mature brain, but nothing is known concerning the localization of both enzymes during development. We have combined in situ hybridization and histochemistry to localize MAOA and MAOB in the developing nervous system of mice. Our observations can be summarized as five key features. (1) MAOA is tightly linked to catecholaminergic traits. MAOA is expressed in all noradrenergic and adrenergic neurons early on, and in several dopaminergic cell groups such as the substantia nigra. MAOA is also expressed in all the neurons that display a transient tyrosine hydroxylase expression in the brainstem and the amygdala and in neurons with transient dopamine-beta-hydroxylase expression in the cranial sensory ganglia. (2) MAOA and MAOB are coexpressed in the serotoninergic neurons of the raphe from E12 to P7. During postnatal life, MAOA expression declines, whereas MAOB expression remains stable. (3) MAOA is transiently expressed in the cholinergic motor nuclei of the hindbrain, and MAOB is expressed in the forebrain cholinergic neurons. (4) MAOA- and MAOB-expressing neurons are also detected in structures that do not contain aminergic neurons, such as the thalamus, hippocampus, and claustrum. (5) Starting at birth, MAOB expression is found in a variety of nonneuronal cells, the choroid plexus, the ependyma, and astrocytes. These localizations are of importance for understanding the effects of monoaminergic transmission during development.

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Section: Methodsmentioning

confidence: 99%

Developmental expression of monoamine oxidases A and B in the central and peripheral nervous systems of the mouse

Vitalis

Fouquet

Alvarez

et al. 2002

J of Comparative Neurology

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“…Serial sections were cut (50 -60 m) on a freezing microtome, collected in cold PB, and processed immediately. MAO activities were revealed according to Dunning et al (1997). Briefly, sections were rinsed three times for 10 minutes each in PB; preincubated for 15 minutes in 50 mM Tris, pH 7.6; incubated for 4 -24 hours at 22-25°C, 0.07% tyramine HCl (Sigma-RBI), 0.005% diaminobenzidine HCl, 0.05% HRP type II (Sigma-RBI), 0.065% sodium azide, 0.6% nickel sulfamate, 50 mM Tris, pH 7.6.…”

Section: Histochemistry Of Maoa and Maob Activitiesmentioning

confidence: 99%

Developmental expression pattern of monoamine oxidases in sensory organs and neural crest derivatives

Vitalis

Alvarez

Chen

et al. 2003

J of Comparative Neurology

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Serotonin (5-HT) has been shown to act as a morphogen in craniofacial and heart development and in the migration of neural crest derivatives. Some of these structures are capable of capturing 5-HT during development, but nothing is known about the localization of the main monoamine degradation enzymes, monoamine oxidase (MAO) A and B, in these developing tissues. We generated a highly specific antibody to MAOB; immunoreactivity is entirely abolished in brain extracts or brain sections of mice lacking MAOB. From the use of this antibody and specific riboprobes, we report that MAOB is expressed early in a variety of neural crest derivatives, in facial sensory organs, and in the heart. From E11.5 to P0, MAOB was found to be strongly expressed in the following neural crest derivatives: the aorta, cranial mesenchyme (developing bones, sensory neurons of the cranial ganglia, cartilages, thyroid, and striate muscles), dental mesenchyme, several soft palate derivatives, and boundary cap cells (E11.5-P4). Boundary cap cells contribute to the formation of nerve exit-entry points between the central and the peripheral nervous systems. Several facial sensory organs also contained MAOB mRNA, protein, and activity. High MAOB expression was noted in the olfactory placode, the dorsal part of the olfactory epithelium, the olfactory nerve layer (probably the ensheathing glia), the cochlear ganglionic cells, the taste buds, and the Merkel cells in the vibrissae follicles. Finally, we found that MAOB is massively expressed in the pharyngeal organ, heart, liver, and mast cells. In contrast, MAOA expression was restricted to the sympathetic ganglia and to the meningeal and capillary blood vessels. The pattern of MAOB expression generally matched the previously reported patterns of expression of the plasma 5-HT transporter expression or of the histamine biosynthetic enzyme L-histidine decarboxylase, suggesting a role for MAOB in fine regulation of the levels of 5-HT and histamine in the developing embryo.

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A Simple Enzyme Histochemical Method for the Simultaneous Demonstration of Acetylcholinesterase and Monoamine Oxidase in Fixed-Frozen Sections

Cited by 2 publications

References 52 publications

Developmental expression of monoamine oxidases A and B in the central and peripheral nervous systems of the mouse

Developmental expression of monoamine oxidases A and B in the central and peripheral nervous systems of the mouse

Developmental expression pattern of monoamine oxidases in sensory organs and neural crest derivatives

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