A simple experiment demonstrating the allosteric regulation of yeast pyruvate kinase

Taber, Richard L.; Campbell, Angela; Spencer, Scott S.

doi:10.1016/s0307-4412(97)00117-9

Cited by 8 publications

(9 citation statements)

References 5 publications

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“…Using a coupled enzyme assay we confirmed that recombinant CpPyK used for crystallization was enzymatically active [37]. In this assay P. falciparum lactate dehydrogenase ( Pf LDH) was used to couple the oxidation of pyruvic acid generated in the reaction catalyzed by CpPyK.…”

Section: Methodsmentioning

confidence: 72%

Crystal Structure of Cryptosporidium parvum Pyruvate Kinase

et al. 2012

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Pyruvate kinase plays a critical role in cellular metabolism of glucose by serving as a major regulator of glycolysis. This tetrameric enzyme is allosterically regulated by different effector molecules, mainly phosphosugars. In response to binding of effector molecules and substrates, significant structural changes have been identified in various pyruvate kinase structures. Pyruvate kinase of Cryptosporidium parvum is exceptional among known enzymes of protozoan origin in that it exhibits no allosteric property in the presence of commonly known effector molecules. The crystal structure of pyruvate kinase from C. parvum has been solved by molecular replacement techniques and refined to 2.5 Å resolution. In the active site a glycerol molecule is located near the γ-phosphate site of ATP, and the protein structure displays a partially closed active site. However, unlike other structures where the active site is closed, the α6' helix in C. parvum pyruvate kinase unwinds and assumes an extended conformation. In the crystal structure a sulfate ion is found at a site that is occupied by a phosphate of the effector molecule in many pyruvate kinase structures. A new feature of the C. parvum pyruvate kinase structure is the presence of a disulfide bond cross-linking the two monomers in the asymmetric unit. The disulfide bond is formed between cysteine residue 26 in the short N-helix of one monomer with cysteine residue 312 in a long helix (residues 303–320) of the second monomer at the interface of these monomers. Both cysteine residues are unique to C. parvum, and the disulfide bond remained intact in a reduced environment. However, the significance of this bond, if any, remains unknown at this time.

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Section: Methodsmentioning

confidence: 72%

Crystal Structure of Cryptosporidium parvum Pyruvate Kinase

et al. 2012

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“…At the same time, the expression of the gene encoding the PK decreases as a physiological response to prevent a futile cycling within glycolysis and gluconeogenesis which may lead to the corresponding energy waste. Previous studies have shown inactivation of pyruvate kinase during gluconeogenesis, to avoid unnecessary energy outlay (Smart & Pritchard, ; Taber, Campbell & Spencer, ; Xu et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown inactivation of pyruvate kinase during gluconeogenesis, to avoid unnecessary energy outlay (Smart & Pritchard, 1982;Taber, Campbell & Spencer, 1998;Xu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profile of pyruvate kinase and pancreatic lipase encoding mRNAs of the Pacific whiteleg shrimpPenaeus vannameiduring PstDV-1 infection

et al. 2017

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Beyond their ability to infect and spread, viruses lack the ability to replicate by their own. To counter this, viruses have evolved strategies to exploit the host's machinery for the production of new virions. However, viruses are by no means merely passive consumers of host metabolic products. Viruses induce remarkable changes in their host's cellular metabolism, yielding a metabolic state, to meet its specific requirements. The decapod penstyldensovirus (PstDV‐1) is probably the most prevalent virus affecting shrimp farming and has been associated with massive mortality outbreaks in hatchery‐reared larvae and juveniles of Penaeus stylirostris, and results in developmental deformities in symptomatic specimens of P. vannamei. Previous studies have suggested that PstDV‐1 induces metabolic reprogramming of P. vannamei to achieve a successful replication. In this study, the effects of PstDV‐1 infection over the gene expression of pyruvate kinase and pancreatic lipase of the shrimp P. vannamei were evaluated. The expression of both genes was significantly altered by PstDV‐1 infection, which may lead to the accumulation of specific metabolites, as lactate and fatty acids, providing a suitable platform for viral assembly and replication. The transcriptional profile of pyruvate kinase and pancreatic lipase‐encoding mRNAs offers initial clues on the potential metabolic alteration that contribute to PstDV‐1 pathogenesis.

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“…Demand for BCAAs as energy metabolites or for the synthesis of immune-related molecules has been demonstrated to increase during disease [76,77]. Decreased levels of a number of other amino acids during Vibrio infection in mussels have also been reported, suggesting that the significantly higher abundance of Alanine aminotransferase (GPT2), Aspartate aminotransferase (GOT1), and Excitatory aminoacid transporter-1 (SLC1A1) in DIS clams may also be associated with the degradation and/or conversion of amino acids into metabolites such as pyruvate, oxaloacetate, and α-ketoglutarate for the TCA cycle [78][79][80][81].…”

Section: Amino Acid Degradation For Energy Productionmentioning

confidence: 99%

A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum

Smits

Artigaud

Bernay

et al. 2020

Fish & Shellfish Immunology

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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A simple experiment demonstrating the allosteric regulation of yeast pyruvate kinase

Cited by 8 publications

References 5 publications

Crystal Structure of Cryptosporidium parvum Pyruvate Kinase

Crystal Structure of Cryptosporidium parvum Pyruvate Kinase

Transcriptional profile of pyruvate kinase and pancreatic lipase encoding mRNAs of the Pacific whiteleg shrimpPenaeus vannameiduring PstDV-1 infection

A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum

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