A Simple FIA-System for Simultaneous Measurements of Glucose and Lactate with Amperometric Detection

Marzouk, Sayed A.M.; Sayour, Hossam E. M.; Ragab, Ahmed; Cascio, Wayne E.; Hassan, Saad S. M.

doi:10.1002/1521-4109(200011)12:16<1304::aid-elan1304>3.0.co;2-b

Cited by 20 publications

(13 citation statements)

References 40 publications

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“…Marzouk et al described a simple flow injection system for simultaneous amperometric determination of glucose and lactate by using two parallel Pt/PmPD-GOx and Pt/PmPD-LAOx electrodes. 429 The reliability of the system assessed by measuring glucose and lactate simultaneously in several samples of blood plasma, urine, and dairy products was in good agreement with those obtained by standard spectrophotometric methods. The flow system proved to offer advantages of (1) simplicity, (2) operational stability, up to 3 months, (3) simultaneous determination of glucose and lactate using a single 20 mL sample injection, (4) stability of the signal of a detective component by wide variations in the concentration of a nondetective component, (5) high sample throughput, more than 50 samples/h, (6) high accuracy and precision, and (7) a reasonable dynamic range of about two orders of magnitude.…”

Section: Bifunctional Biosensorsupporting

confidence: 53%

Novel Multifunctional Polymers from Aromatic Diamines by Oxidative Polymerizations

et al. 2002

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Section: Bifunctional Biosensorsupporting

confidence: 53%

Novel Multifunctional Polymers from Aromatic Diamines by Oxidative Polymerizations

et al. 2002

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“…Having described the AA sensitivity of PoPD-based polymers in some detail, the behaviour of polymers synthesised from monomers related to oPD can be condensed into the four AA calibration parameters given in Table 1. The meta isomer, mPD, was tested because it has been used recently by several laboratories in biosensor applications, [68][69][70][71][72][73][74] and to compare with our previous Pt/PmPD/GOx results. 26 The values of both I max (p < 0.001) and I lim (p < 0.0001) were significantly greater for Pt/ PmPD/BSA compared with Pt/PoPD/BSA and supports our previous conclusion, based on PPD/GOx electrodes, 26 that oPD is the superior isomer for biosensor applications.…”

Section: Pt/pmpd/bsa Electrodesmentioning

confidence: 99%

Comparison of simple aromatic amines for electrosynthesis of permselective polymers in biosensor fabrication

Craig

O’Neill

2003

Analyst

101

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A range of simple aromatic amines were used to modify Pt microcylinders with insulating electrosynthesised polymers generated amperometrically in the presence or absence of a typical globular protein, bovine serum albumin (BSA), at neutral pH. Sensitivity to a typical interference species, ascorbic acid (AA), and the most common electrochemical signal molecule for oxidase enzymes, H 2 O 2 , was used to compare the suitability of the resulting polymer-protein modified electrodes for biosensor applications. Pt modified with poly(aminobenzene), PANI, gave the lowest sensitivity to AA ( ~3000 times lower than bare Pt); incorporation of BSA into the polymer during synthesis had a detrimental effect on its AA-rejecting capability. The H 2 O 2 sensitivity of the PANI-based electrodes was poor ( ~7 times lower) compared to bare Pt. The behaviour of poly(1,2-diaminobenzene), PoPD, was different in many respects: its AA-blocking ability was enhanced 3-fold by the presence of BSA and its H 2 O 2 sensitivity was similar to bare Pt. EQCM data recorded in PBS indicated that PANI films electrosynthesised at pH 7.4 were two orders of magnitude thicker than for PoPD. These differences may be due, at least in part, to the 'ladder' structure with phenazine rings proposed for PoPD, since they were not duplicated by other mono-substituted derivatives of aniline, such as 2-methylaniline or 1,3-diaminobenzene.

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“…Moreover, the biosensors based on the enzymatic reactors possess the distinctive advantage of much larger amount of the immobilized enzyme resulting in the higher stability of the biosensor and lower enzyme consumption in general. The development of the flow amperometric biosensors with enzyme‐based reactors for the determination of lactate , glucose , sucrose , ascorbic acid , and hydrogen peroxide has been recently reported. To our knowledge, only one biosensor with enzymatic reactor for the determination of Ch has been proposed , which is based on covalently immobilized ChOx on an NH 2 ‐functionalized controlled pore glass support as a reactor and a Pt electrode modified by electrosynthesized polypyrrole film as a transducer for direct H 2 O 2 detection in FIA.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Biosensor Based on Enzymatic Reactor for Choline Determination in Flow Systems

2019

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A simple, selective and stable biosensor with the enzymatic reactor based on choline oxidase (ChOx) was developed and applied for the determination of choline (Ch) in flow injection analysis with amperometric detection. The enzyme ChOx was covalently immobilized with glutaraldehyde to mesoporous silica powder (SBA‐15) previously covered by NH2‐groups. This powder was found as an optimal filling of the reactor. The detection of Ch is based on amperometric monitoring of consumed oxygen during the enzymatic reaction, which is directly proportional to Ch concentration. Two arrangements of an electrolytic cell in FIA, namely wall‐jet cell with working silver solid amalgam electrode covered by mercury film and flow‐through cell with tubular detector of polished silver solid amalgam were compared. The experimental parameters affecting the sensitivity and stability of the biosensor (i. e. pH of the carrier solution, volume of reactor, amount of the immobilized enzyme, the detection potential, flow rate, etc.) were optimized. Under the optimized conditions, the limit of detection was found to be 9.0×10−6 mol L−1. The Michaelis‐Menten constant for covalently immobilized ChOx on SBA‐15 was calculated. The proposed amperometric biosensor with the developed ChOx‐based reactor exhibits good repeatability, reproducibility, long‐term stability, and reusability. Its efficiency has been confirmed by the successful application for the determination of Ch in two commercial pharmaceuticals.

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A Simple FIA-System for Simultaneous Measurements of Glucose and Lactate with Amperometric Detection

Cited by 20 publications

References 40 publications

Novel Multifunctional Polymers from Aromatic Diamines by Oxidative Polymerizations

Novel Multifunctional Polymers from Aromatic Diamines by Oxidative Polymerizations

Comparison of simple aromatic amines for electrosynthesis of permselective polymers in biosensor fabrication

Amperometric Biosensor Based on Enzymatic Reactor for Choline Determination in Flow Systems

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