A simple fluorometric method for rapid screening of aflatoxins after their extraction by magnetic MOF-808/graphene oxide composite and their discrimination by HPLC

Alilou, Sevda; Amirzehni, Maliheh; Eslami, Parvin Alizadeh

doi:10.1016/j.talanta.2021.122709

Cited by 45 publications

(6 citation statements)

References 28 publications

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“…In the last few years, there has been an exponential growth in research works regarding the development of molecularly imprinted polymers (MIPs) for mycotoxins analysis [ 19 , 20 ]. The effectiveness of MIPs relies on the formation of nanostructured binding sites in organic polymers, which are obtained using different strategies.…”

Section: Introductionmentioning

confidence: 99%

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Palmieri

Elfadil²,

Fanti³

et al. 2023

Molecules

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Aflatoxins (AFs) are fungi secondary metabolites produced by the Aspergillus family. These compounds can enter the food chain through food contamination, representing a risk to human health. Commercial immunoaffinity columns are widely used for the extraction and cleanup of AFs from food samples; however, their high cost and large solvent consumption create a need for alternative strategies. In this work, an alternative strategy for producing molecularly imprinted polymers (MIPs) was proposed to extract aflatoxins AFB1, AFB2, AFG1, and AFG2 from complex food samples, using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). The MIPs were synthesized via a low-cost and rapid (5 min) sonochemical free-radical polymerization, using 1-hydroxy-2-naphthoic acid as a dummy template. MIPs-based solid phase extraction performance was tested on 17 dietary supplements (vegetables, fruits, and cereals), obtaining appreciable recovery rates (65–90%) and good reproducibility (RSD ≤ 6%, n = 3); the selectivity towards other mycotoxins was proved and the data obtained compared with commercial immunoaffinity columns. The proposed strategy can be considered an alternative affordable approach to the classical immunoaffinity columns, since it is more selective and better performing.

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Section: Introductionmentioning

confidence: 99%

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Palmieri

Elfadil²,

Fanti³

et al. 2023

Molecules

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“…However, this procedure has not only been studied for liquid or oily food samples, other agri-food matrices including maize, wheat, pistachio or fruit samples have been tested using different magnetic nanomaterials [ 11 , 14 , 15 , 16 , 17 , 18 ]. In addition, two types of nanomaterials synthesized using ionic liquids for B1, B2, G1 and G2 AFs determination in milk and pistachio samples [ 19 , 20 ] and an imprinted molecular polymer [ 21 ] in milk, rice and wheat flour samples have been used for the determination of the four major aflatoxins together with AFM1 and AFM2 with the DMSPE procedure. All these studies were carried out via a targeted approach, clearly pointing out the lack of other studies also doing non-targeted analysis in order to assess the presence of other aflatoxin-derived metabolites.…”

Section: Introductionmentioning

confidence: 99%

Dispersive Magnetic Solid-Phase Extraction as a Novelty Sample Treatment for the Determination of the Main Aflatoxins in Paprika

García-Nicolás

Arroyo‐Manzanares

Viñas

2023

Toxins

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Dispersive magnetic solid-phase extraction (DMSPE) technique is proposed as a new sensitive and effective sample treatment method for the determination of aflatoxins in paprika samples. DMSPE was followed by ultrahigh-performance liquid chromatography and high-resolution mass spectrometry detection (UHPLC-HRMS) using a non-targeted acquisition mode for the detection of main aflatoxins (aflatoxin G1, G2, B1 and B2) and derivatives. DMSPE was based on the use of magnetic nanocomposite coated with polypyrrole (PPy) polymer and the main experimental parameters influencing the extraction efficiency in adsorption and desorption steps have been studied and optimized. Analyses were performed using 250 µL magnetic PPy nanocomposite into the sample solution, adsorbing the analytes in 30 min and desorbing them with ethyl acetate (2 mL) in 15 min. The method has been validated, obtaining quantification limits between 3.5 and 4.7 µg kg−1 and recoveries between 89.5–97.7%. The high recovery rate, wide detection range and the use for the first time of the reusable Fe3O4@PPy nanomaterial in suspension for solid food matrices, guarantee the usefulness of the method developed for adequate control of aflatoxins levels in paprika. The proposed methodology was applied for the analysis of 31 samples (conventional and organic) revealing the absence of aflatoxins in the samples.

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“…MIPs prepared by traditional bulk polymerization method have shortages of irregular particle morphology, deeply embedded templates, and slow mass transfer speed, − which seriously affect their specific recognition capability to the target molecules. Surface molecular imprinting technology supplies a way to overcome above shortages by growing a thin MIP layer onto a suitable supporting carrier with regular structures, e.g., silica spheres. , As the imprinted layers are thin, the templates can be easily removed, and the remaining imprinted cavities is close to the surface which is beneficial to improve the mass transfer efficiency. ,, On the basis of the surface molecular imprinting technology, various MIPs targeting AFB 1 were developed based on different materials: creation of an MIP layer on mesoporous silicas and carbon dots for selective extraction of AFB 1 in food samples which was further analyzed by high-performance liquid chromatography (HPLC), giving a limit of detection (LOD) of 0.118 ng/mL; coating MIP onto the gold chip to make surface plasmon resonance (SPR) sensor with a low LOD of 1.04 pg/mL; grafting MIP recognition sites onto quantum dots , and metal–organic frameworks , to prepare optical sensors with LODs in the level of ng/mL. In general, a dummy template imprinting strategy was mostly employed, e.g., using 5,7-dimethoxycoumarin (DMC) as the template instead of AFB 1 itself, due to pure AFB 1 being expensive and toxic.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Ordered Macroporous Magnetic Inverse Photonic Crystal Microsphere-Based Molecularly Imprinted Polymer for Selective Capture of Aflatoxin B₁

Wang

Shao

et al. 2022

ACS Appl. Mater. Interfaces

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Development of an efficient detection method to monitor residual mycotoxins in food is very important to ensure food safety, but the complex food matrix seriously affects the detection sensitivity and accuracy. Here, using a three-dimensional ordered macroporous magnetic inverse photonic crystal microsphere (MPCM) as the supporting material, a molecularly imprinted polymer (MIP) that can selectively recognize aflatoxin B1 (AFB1) was synthesized through the dummy template imprinting strategy. The MPCM@MIP prepared by employing 5,7-dimethoxycoumarin as the template and methacrylic acid as the functional monomer displayed selectivity toward AFB1 (imprinting factor of 1.5) and could be used as a solid-phase extraction material. By coupling with high-performance liquid chromatography, an analytical method targeting AFB1 was established and displayed a wide linear range of 5–1000 ng/mL with a low detection limit of 0.4 ng/mL. The method showed a good recovery rate of 73–92% in AFB1-spiked soy sauce and vinegar samples. Moreover, the MPCM@MIP could be separated from the sample solution easily because of its magnetic performance, displaying a promising future not only in the enrichment of AFB1 to improve the detection sensitivity and accuracy but also in the removal of AFB1 from food and environmental samples.

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A simple fluorometric method for rapid screening of aflatoxins after their extraction by magnetic MOF-808/graphene oxide composite and their discrimination by HPLC

Cited by 45 publications

References 28 publications

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Dispersive Magnetic Solid-Phase Extraction as a Novelty Sample Treatment for the Determination of the Main Aflatoxins in Paprika

Three-Dimensional Ordered Macroporous Magnetic Inverse Photonic Crystal Microsphere-Based Molecularly Imprinted Polymer for Selective Capture of Aflatoxin B₁

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A simple fluorometric method for rapid screening of aflatoxins after their extraction by magnetic MOF-808/graphene oxide composite and their discrimination by HPLC

Cited by 45 publications

References 28 publications

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Dispersive Magnetic Solid-Phase Extraction as a Novelty Sample Treatment for the Determination of the Main Aflatoxins in Paprika

Three-Dimensional Ordered Macroporous Magnetic Inverse Photonic Crystal Microsphere-Based Molecularly Imprinted Polymer for Selective Capture of Aflatoxin B1

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Three-Dimensional Ordered Macroporous Magnetic Inverse Photonic Crystal Microsphere-Based Molecularly Imprinted Polymer for Selective Capture of Aflatoxin B₁