A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain

Jîtcă, George; Fogarasi, Erzsébet; Ősz, Bianca-Eugenia; Vari, Camil-Eugen; Tero-Vescan, Amelia; Miklos, Amalia; Bătrînu, Mădălina-Georgiana; Rusz, Carmen Maria; Croitoru, Mircea Dumitru; Dogaru, Maria Titica

doi:10.3390/molecules26165066

Cited by 8 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following the heating process, the samples were transferred in HPLC vials and promptly analyzed following the derivatization reaction. For the analysis of plasma samples, a similar procedure was followed, with strict modifications [ 56 ]. Representative chromatograms of plasma and brain MDA analysis are shown in Supplementary Figures S1 and S2 .…”

Section: Methodsmentioning

confidence: 99%

Drug Repurposing of Metformin for the Treatment of Haloperidol-Related Behavior Disorders and Oxidative Stress: A Preliminary Study

Jîtcă,

Gáll,

Jîtcă

et al. 2024

Pharmaceutics

Self Cite

View full text Add to dashboard Cite

A particular attribute of the brain lies in the ability to learn, acquire information from the environment, and utilize the learned information. Previous research has noted that various factors (e.g., age, stress, anxiety, pathological issues), including antipsychotic medications, affect the brain and memory. The current study aimed to reveal the effects of chronic metformin treatment on the cognitive performance of rats and on commonly measured markers for oxidative stress. Wistar male rats (n = 40) were randomly divided into four groups: CTR (n = 10)–control group, METF (n = 10)–animals receiving metformin 500 mg/kg, HAL (n = 10)–animals receiving haloperidol 2 mg/kg, and HALMETF (n = 10)–animals receiving haloperidol 2 mg/kg and metformin 500 mg/kg. The medication was administered daily by oral gavage for 40 days. Memory and learning were assessed using the Morris Water Maze (MWM) test. At the end of the MWM, the rodents were decapitated under anesthesia, and the brain and blood samples were assayed by liquid chromatography for markers of oxidative stress (malondialdehyde, MDA, reduced/oxidized glutathione ratio, GSH/GSSG). The quantification of brain-derived neurotrophic factor (BDNF) was performed using the conventional sandwich ELISA technique. In the HALMETF group, metformin attenuated the negative effects of haloperidol. Brain and plasma MDA levels increased in the HAL group. Brain and plasma GSH/GSSG ratios and BDNF levels did not reveal any differences between groups. In conclusion, metformin treatment limits the deleterious cognitive effects of haloperidol. The effect on oxidative stress markers may also point toward an antioxidant-like effect of metformin, but this needs further tests for confirmation.

show abstract

Section: Methodsmentioning

confidence: 99%

Drug Repurposing of Metformin for the Treatment of Haloperidol-Related Behavior Disorders and Oxidative Stress: A Preliminary Study

Jîtcă,

Gáll,

Jîtcă

et al. 2024

Pharmaceutics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Так, деякими вченими зареєстровано, що при стресі вміст продуктів перекисного окиснення ліпідів зростає: концентрація дієнових кон'югатів на 10,6%, а МДА -на 11,7% [22]. Отримані в ході інших досліджень аналітичні кореляційні криві для МДА в головному мозку також були лінійними, а концентрація перебувала в нормальному діапазоні (0,2-20 мкг/г, коефіцієнт регресії =0,998) [23].…”

Section: параметрunclassified

Clinical course of pro-oxidant processes in brain structures in rats under conditions of experimental hyperthyroxinemia in age-related dynamics

Voichenko,

Rodynskyi

2023

Med. perspekt.

View full text Add to dashboard Cite

The search for a connection between changes in the indicators of the pro-oxidant system of brain structures and the thyroid gland as a target organ is an urgent issue. The goal is to investigate the features of the pro-oxidant system against the background of experimental hyperthyroxinemia and to evaluate the age-related changes of malondialdehyde and diene conjugates in brain structures. Among Wistar rats (n=50) intact animals ("control", Σ=20) and experimental animals (under simulated hyperthyroxinemia, Σ=30) were selected for observation. The principle of the model of experimental hyperthyroxinemia lies in the addition of levothyroxine in a dosage of 20 μg/day (manufactured by "Berlin-Chemie AG", Germany) in the form of tablets with subsequent grinding at 9 o'clock in the morning with food. On day 14, hyperthyroxinemia in the animals was found, while the thyroxine level in the peripheral blood was >10 μg/dL in the first 48 hours and further, being >25 μg/dL (the results were verified in the laboratory, confirmed by immunoenzymatic method with the participation of the "Humarlander" test system). After functional testing in rats, highly specific parameters (malondialdehyde, diene conjugates) in the cortex, trunk, and hippocampus were further recorded and evaluated. The humane treatment of animals is certified by the local protocol of the meeting of the commission of the Dnipro State Medical University No. 10 dated 21.06.2023, bioethical principles and rules are taken into account during the experimental research. In the presented work, the results of the biochemical method were used to discuss the importance of the pro-oxidant system at the stage of protection against manifestations of stress and anxiety. Functional dependence on the accumulation of intermediate and final products of lipid peroxidation in the structures of the brain during the study of the state of the pro-oxidant system was found; the age-related dynamics of the activity of malondialdehyde and diene conjugates from young to middle-aged rats under conditions of experimental hyperthyroxinemia was observed; age-related changes contribute to a certain depletion of the activity of pro-oxidant compounds in response to stress as a principle of nervous system protection.

show abstract

“…This decrease in GSH is most likely due to methanol metabolites. In fact, multiple studies have demonstrated this oxidative damage, both at the hepatocellular level and in the kidneys, but, most likely, there are oxidative lesions at the central level as well [ 142 , 145 , 146 , 147 , 148 , 149 ]. In the same manner, a reduction in protein thiols was observed, which resulted from the oxidation of thiolic groups (-SH), which was the result of the decrease in GSH [ 142 , 145 ].…”

Section: From Pure Caffeine To Coffee Productsmentioning

confidence: 99%

Caffeine and Its Antioxidant Properties—It Is All about Dose and Source

Ősz

Jîtcă²,

Ștefănescu³

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Caffeine is the most frequently used substance with a central nervous system stimulant effect, but its consumption is most often due to the intake of foods and drinks that contain it (coffee, tea, chocolate, food supplements with plant extracts of Guarana, Mate herba, Cola nuts). Due to its innocuity, caffeine is a safe xanthine alkaloid for human consumption in a wide range of doses, being used for its central nervous stimulating effect, lipolytic and diuresis-enhancing properties, but also as a permitted ergogenic compound in athletes. In addition to the mechanisms that explain the effects of caffeine on the targeted organ, there are many proposed mechanisms by which this substance would have antioxidant effects. As such, its consumption prevents the occurrence/progression of certain neurodegenerative diseases as well as other medical conditions associated with increased levels of reactive oxygen or nitrogen species. However, most studies that have assessed the beneficial effects of caffeine have used pure caffeine. The question, therefore, arises whether the daily intake of caffeine from food or drink has similar benefits, considering that in foods or drinks with a high caffeine content, there are other substances that could interfere with this action, either by potentiating or decreasing its antioxidant capacity. Natural sources of caffeine often combine plant polyphenols (phenol-carboxylic acids, catechins) with known antioxidant effects; however, stimulant drinks and dietary supplements often contain sugars or artificial sweeteners that can significantly reduce the effects of caffeine on oxidative stress. The objective of this review is to clarify the effects of caffeine in modulating oxidative stress and assess these benefits, considering the source and the dose administered.

show abstract

A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain

Cited by 8 publications

References 24 publications

Drug Repurposing of Metformin for the Treatment of Haloperidol-Related Behavior Disorders and Oxidative Stress: A Preliminary Study

Drug Repurposing of Metformin for the Treatment of Haloperidol-Related Behavior Disorders and Oxidative Stress: A Preliminary Study

Clinical course of pro-oxidant processes in brain structures in rats under conditions of experimental hyperthyroxinemia in age-related dynamics

Caffeine and Its Antioxidant Properties—It Is All about Dose and Source

Contact Info

Product

Resources

About