A Simple HPLC Method with Fluorescence Detection for Simultaneous Determination of 10-methoxycamptothecin and its Metabolite 10-hydroxycamptothecin in Rat Liver Tissue

Abstract

A simple HPLC method to determine the amount of 10-methoxycamptothecin (MCPT) and its major metabolite 10-hydroxycamptothecin (HCPT) in rat liver tissue was developed in the present study. Camptothecin (CPT) was used as internal standard (IS). A piecewise linear function was used over lower and higher concentrations, respectively. The calibration curves were linear (r (2) >0.99) over concentrations from 2.5 to 20 ng/mL and 20 to 320 ng/mL for both MCPT and HCPT. The method had an accuracy of 92.74% to 112.76%, and the intra- and inter-day precision (RSD%) were 11.85% or less for MCPT and HCPT. The stability data showed no significant degradation occurred under the experimental conditions. This method was successfully applied to the tissue distribution study of MCPT and its metabolite HCPT in liver tissue samples after intravenous administration.

Tissue distribution of 10-methoxycamptothecin and its metabolite 10-hydroxycamptothecin in rats by a RP-HPLC method with fluorescence detection/UV detection

“…21 In present study, the necessary validation, including selectivity, linearity and extraction recoveries were investigated for lung, kidney, heart, spleen, and brain tissue homogenate samples. 21 In present study, the necessary validation, including selectivity, linearity and extraction recoveries were investigated for lung, kidney, heart, spleen, and brain tissue homogenate samples.…”

“…injection. 21 The mobile phase was composed of water (containing 5% ACN) as mobile phase A and ACN as mobile phase B. 11 In order to improve the understanding the in vivo behavior of MCPT, the study on the tissue distribution of MCPT and its major metabolite HCPT is necessary.…”

“…A validated HPLC method using a uorescence detector was established for the quantication of MCPT and HCPT in liver tissue in our previous study. 21 In the present study, the method coupled with a uorescence detector (or a UV detector) based on protein precipitation was applied to simultaneously determine MCPT and HCPT in other tissues with essential validation and the distribution patterns in liver, kidney, lung, heart, spleen and brain were then illustrated. Surprisingly, MCPT was overwhelmingly accumulated in the lung tissues and the concentration was up to 1000 times higher than that found in the plasma sample.…”

Synthesis, antitumor activity and pharmacokinetic study of 10‐propionyloxy camptothecin in rats

“…The binary gradient mobile phases were water (containing 5% ACN) as mobile phase A and ACN as mobile phase B. The time program of the gradient was the same as for our previous studies (Liu et al, 2014;Shao et al, 2015;. The total data acquisition time was 25 min.…”

Combined use of ionic liquid and sulfobutylether‐β‐cyclodextrin as electrolyte additives for separation and determination of camptothecin alkaloids by CZE

“…For the past decades, some analytical methods for separation and determination of CPT alkaloids have been developed, including HPLC , TLC , fluorimetric analysis method , and high‐performance CE . Among these detection systems, high‐performance CE has been increasingly applied to the separation and determination of the traditional Chinese medicine herbs owing to its high speed, high efficiency, ultra small sample volume used, and ease of cleaning up contaminants.…”