A Simple Immunofluorescence Test for the Detection of Platelet Antibodies

Borne, A E von dem; Verheugt, Freek W. A.; Oosterhof, F.; Riesz, E; Rivière, Aart Brutel de la; Engelfriet, C. P.

doi:10.1111/j.1365-2141.1978.tb01089.x

Cited by 565 publications

(80 citation statements)

References 13 publications

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“…The alloantibody level decreased progressively and was undetectable 3 months later by both ELISA 14 and immunofluorescence. 10 Nevertheless, because of the risk of rebound if platelet transfusions were needed, and since she had a healthy HLA-identical brother, BMT was suggested.…”

Section: Casementioning

confidence: 99%

Bone marrow transplantation in severe Glanzmann's thrombasthenia with antiplatelet alloimmunization

Bellucci

Damaj

Boval

et al. 2000

Bone Marrow Transplant

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Summary:Glanzmann's thrombasthenia is an autosomal recessive disorder characterized by a lack of platelet aggregation due to the absence of platelet glycoprotein IIb and IIIa. Usually, the disease leads to mild hemorrhage but sometimes bleeding is severe enough to be life-threatening. We report the case of a 16-year-old girl, presenting with very severe type 1 Glanzmann's thrombasthenia, successfully treated with an HLA-identical sibling bone marrow transplant (BMT). We also update the clinical and laboratory data of her brother, who had received a BMT 16 years ago for the same disease. In the light of these two cases and two others published in the literature, we discuss the indications for BMT from HLAidentical sibling donors in Glanzmann's thrombasthenia. Alloimmunization against the missing platelet GPIIb/IIIa complex and severity of bleeding episodes may constitute sufficient criteria for allogeneic BMT after careful assessment of the risk-benefit of such a procedure, although this remains exceptional in this disease. Bone Marrow Transplantation (2000) 25, 327-330.

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Section: Casementioning

confidence: 99%

Bone marrow transplantation in severe Glanzmann's thrombasthenia with antiplatelet alloimmunization

Bellucci

Damaj

Boval

et al. 2000

Bone Marrow Transplant

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“…Platelet indirect immunofluorescent test (PIFT) was performed by the recommended technique. 5 The father's platelets were incubated in one tube with the maternal serum and in another tube with the infant's serum. A second layer of fluorescein isothiocyanate-labelled mouse anti-human IgG was added.…”

Section: Detection Of Anti-platelet and Anti-hla Antibodiesmentioning

confidence: 99%

A Rare Case of Neonatal Alloimmune Thrombocytopenia Due to Anti-HPA-2b

et al. 1998

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Despite the fact that neonatal alloimmune thrombocytopenia (NAIT) is a relatively rare disease (1/1500-1/5000 live births), 1 its importance stems from its potential to cause serious complications. NAIT accounts for 20% of cases of neonatal thrombocytopenia and is most commonly attributed to platelet-specific antigens. Diagnosis is usually confirmed by demonstrating that antiplatelet antibodies in the mother's serum are reactive with her baby's or husband's platelets. This test is, however, not always reliable.2 Alternatively, platelets from the family could be serotyped, using specific antisera, but this is usually limited to reference laboratories. Recently, it has become possible to genotype platelet alloantigens, using various DNA-based techniques.3,4 This does not only simplify diagnosis but also allows early recognition and therapeutic intervention, such as intrauterine platelet transfusion and delivery by cesarean section. These therapeutic measures may prevent mortality or serious sequelae, such as permanent CNS damage. In this case report, we discuss the utilization of polymerase chain reaction-sequence specific primer (PCR-SSP) technology in a Saudi newborn with NAIT. Case ReportA full-term, low birth weight (2.3 kg) baby boy was normally delivered in the Maternity and Children's Hospital, Dammam in 1997. Apgar score was normal and there were no dismorphic features, petechiae or purpuric lesions. Routine biochemical investigations revealed low serum glucose (34 mg/dL). Hematological tests done on the first day showed normal Hb 21.1 g/dL, normal WBC (8.5x109 /L) and low platelet count (100x10 9 /L). The platelet count thereafter dropped to 66x10 9 /L on the 6th day of admission and became normal (163x10 9 /L) two weeks after delivery. Differential WBC count showed a predominance of lymphocytes (54%), with 42% neutrophils, 2% monocytes and 2% eosinophils. Apart from a few nucleated RBC and physiological macrocytosis, RBC morphology was normal. A few platelets looked morphologically large. Coagulation studies excluded disseminated intravascular coagulopathy (DIC). Serological evaluation was negative for toxoplasma, but positive (IgG) for both rubella and cytomegalovirus (CMV), indicating maternally acquired antibodies. Blood culture did not grow any bacteria. The mother had three other healthy children. She aborted twice during her second and fourth pregnancies in the first and second trimesters for unknown etiology. She had a history of hypertension but was not on any medication. There was no history of a similarly affected sibling with thrombocytopenia or bleeding manifestations. The mother herself did not have any history of bleeding. Her platelet count was 216x10 9 /L on the day of the delivery. Detection of Anti-Platelet and Anti-HLA AntibodiesSerum was collected from the baby and the mother, and 10 mL EDTA blood from the father provided platelets for the antibody test. Platelet indirect immunofluorescent test (PIFT) was performed by the recommended technique.5 The father's platelets were incubated i...

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“…All three were associated with the presence of a novel platelet-specific alloantibody that could only be detected by the enzyme-linked immunosorbent assay (ELISA) utilising intact, adherent platelets [12, 13]. An unusual characteristic of this alloantibody was its inability to be detected by the platelet suspension immunofluorescence test (PSIFT) [14]and by the monoclonal antibody immobilisation of platelet antigens assay (MAIPA) [15]. …”

Section: Introductionmentioning

confidence: 99%

Three Cases of Platelet Alloimmunisation Associated with the Presence of a Novel Platelet-Specific Antibody

Masters

Taaning

1998

Vox Sanguinis

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Background and Objectives: In three cases of platelet alloimmunisation, a platelet-specific alloantibody was detected which could not be classified within the known human platelet alloantigen or HLA systems. The first case was of a family in which two siblings suffered neonatal alloimmune thrombocytopenia at birth. In the second case, the newborn was suffering from phocomelia with hypoplastic thrombocytopenia. The third case was a male who became refractory to transfusions of HLA-matched platelets after a related bone marrow transplantation. Materials and Methods: The serum samples were investigated by: enzyme-linked immunosorbent assay, platelet suspension immunofluorescence test (PSIFT), monoclonal antibody immobilisation of platelet antigens assay (MAIPA), and by the lymphocytotoxicity test. Results: The antibody gave positive reactions with 26% of normal donor platelets. Surprisingly, no platelet-specific antibody was detected by PSIFT or by MAIPA and there was no evidence found to support classifying the antibody within the HLA system. Conclusion: The reactivity pattern of the antibody detected and the clinical presentation of the three cases described, strongly suggest the presence of an additional platelet-specific alloantigen system.

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A Simple Immunofluorescence Test for the Detection of Platelet Antibodies

Cited by 565 publications

References 13 publications

Bone marrow transplantation in severe Glanzmann's thrombasthenia with antiplatelet alloimmunization

Bone marrow transplantation in severe Glanzmann's thrombasthenia with antiplatelet alloimmunization

A Rare Case of Neonatal Alloimmune Thrombocytopenia Due to Anti-HPA-2b

Three Cases of Platelet Alloimmunisation Associated with the Presence of a Novel Platelet-Specific Antibody

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