A simple, label-free AuNPs-based colorimetric ultrasensitive detection of nerve agents and highly toxic organophosphate pesticide

Sun, Jingbo; Guo, Lei; Bao, Yi Wang; Xie, Jianwei

doi:10.1016/j.bios.2011.07.012

Cited by 139 publications

(45 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, different NPbased assays with nonconjugated cholinesterases were also developed. For example, colorimetric assays based on growth or aggre gation of gold NPs upon substrate hydrolysis showed better detection limits than 10 −10 M of antiChE concen trations (Periasamy et al, 2009;Wang et al, 2009;Sun et al, 2011;Wang et al, 2013).…”

Section: Copyrights (2003) American Chemical Society and (2008) John mentioning

confidence: 98%

Cholinesterase Inhibitors

Waiskopf¹,

Soreq²

2015

Handbook of Toxicology of Chemical Warfare Agents

View full text Add to dashboard Cite

Section: Copyrights (2003) American Chemical Society and (2008) John mentioning

confidence: 98%

Cholinesterase Inhibitors

Waiskopf¹,

Soreq²

2015

Handbook of Toxicology of Chemical Warfare Agents

View full text Add to dashboard Cite

“…These samples were pretreated by the previous described method (Sun et al 2011). Typically, these fruit juices were treated with acetonitrile for 20 min under vigorous shaking, and then, the insoluble draff was removed by centrifugation.…”

Section: Pretreatment Of the Fruit Juice Samplesmentioning

confidence: 99%

Acetylcholinesterase-Free Colorimetric Detection of Chlorpyrifos in Fruit Juice Based on the Oxidation Reaction of H2O2 with Chlorpyrifos and ABTS2− Catalyzed by Hemin/G-Quadruplex DNAzyme

et al. 2014

View full text Add to dashboard Cite

Herein, we for the first time report an acetylcholinesterase (AChE)-free colorimetric method for the detection of chlorpyrifos (CP), which is based on the oxidation reaction of H 2 O 2 with CP and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS 2− ) catalyzed by hemin/G-quadruplex DNAzyme. In the absence of CP, ABTS 2− is directly oxidized by H 2 O 2 with hemin/Gquadruplex DNAzyme as a biocatalyst to produce the bluegreen-colored free radical anion (ABTS ·− ), accompanied with an obvious UV-visible absorbance at 418 nm. However, the amount of H 2 O 2 used in H 2 O 2 -DNAzyme-ABTS 2− system will be decreased if CP is first reacted with H 2 O 2 , leading to the decrease of the amount of ABTS ·− and a change in the UVvisible absorbance of ABTS ·− at 418 nm. Thus, an indirect dependence relation between CP concentration and the absorbance of ABTS ·− at 418 nm could be constructed and used for CP detection. Under optimal conditions, the detection range of the method is 0.04-1 μg/mL, with a detection limit of 0.01 μg/ mL. Application of the method in real juice samples shows good analytical features in terms of recoveries (95-107.5 %), the relative standard deviation (RSD) (0.6-4.7 %), rendering it as an attractive candidate to current methodologies for the determination of CP.

show abstract

“…The utilization of two kinds of enzymes increases cost that limits their applications. Recently, nanomaterials such as fluorescent conjugated polymers, Fe 3 O 4 nanoparticles [17], gold nanoparticles [18][19][20][21][22][23], graphene quantum dots [24], upconversion nanoparticles [25], DNA-templated copper/silver nanoclusters [26], CdTe quantum dots [27] have also been successfully employed for the detection of AChE activity and its inhibitors. However, most of these nanomaterials-based methods show certain drawbacks, such as labor-intensive and time-consuming sample pretreatment, the use of other enzymes for signal amplification and readout, low sensitivity, and sophisticated instrument manipulation.…”

Section: Introductionmentioning

confidence: 99%