A simple method for acid-extraction of cadmium from tissue

Syversen, Tore; Syversen, Gaby B.

doi:10.1007/bf01684871

Cited by 14 publications

(2 citation statements)

References 3 publications

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“…For analysis of zinc and copper, tissue samples (about 0.2 g) were digested as follows (19). Metal-free nitric acid (1 ml) was added to each sample and maintained at room temperature for at least 24 h. The nitric acid digests were heated to 60" C for 5 min to dissolve small particle.…”

Section: Estimation Of Metallothionein and Metals (Zn And Cu)mentioning

confidence: 99%

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Hepatic Metallothionein as a Source of Zinc and Cysteine during the First Year of Life

Zlotkin

Cherian

1988

Pediatr Res

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ABSTRACT. Metallothionein, a high cysteine-containing protein, can bind with both essential and nonessential metals and thus play an important role as a metal storage protein and also in the detoxification of toxic metals. Although in the human fetus, levels of trace minerals and metallothionein are very high, their postnatal changes are not well documented. The purpose of the present investigation, therefore, was to quantify the accumulation of metallothionein in premature and full-term infants during the first year of life and to identify factors affecting its accumulation. From 47 postmortem samples, it was determined that hepatic metallothionein levels were highest in newborn premature and full-term infants falling to levels found in older children by 4.4 months of age. Hepatic zinc levels were also highest in the youngest infants, falling with increasing postnatal age. There was a significant positive correlation between zinc and metallothionein at all ages. However, there was a negative correlation between hepatic metallothionein levels and cystathionase activity. Hepatic copper and metallothionein levels were unrelated. The renal concentration of metallothionein, zinc, and copper were significantly lower than corresponding hepatic levels. The fall in hepatic levels of zinc and metallothionein during the first months of life correspond to a period of negative zinc balance and low endogenous cysteine production in the newborn. Thus metallothionein may play an important role as a storage depot for these two essential nutrients during this critical period of active growth. (Pediatr Res 24: 326-329, 1988) During the last trimester of pregnancy the mature placenta regulates the transfer of nutrients and protects the fetus from potentially toxic substances. With premature birth, the newborn infant is at risk of both nutrient deficiencies and toxicities for at least five reasons. I) Nutrient intake requirements may be unknown thus too much or too little may be provided; 2) homeostatic mechanisms in the functionally immature preterm are poorly developed; 3) the immature blood-brain bamer is more permeable to any elements present in the plasma; 4) growth is extremely rapid, thus nutrient needs are highest at the same time that nutrient stores are very low; and 5) postnatal adaptation may be prolonged for months even in the absence of overt disease. This combination of factors demand that both environmental and nutritional factors be rigidly controlled for the newborn premature infant.It has been established that most of the minerals of the human fetus are layed down during the last trimester of pregnancy (1).

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Section: Estimation Of Metallothionein and Metals (Zn And Cu)mentioning

confidence: 99%

“…Hepatic copper and metallothionein concentrations were unrelated (r = 0. 19 Metallothionein and cystathionase activity. There was a significant negative correlation between hepatic cystathionase activity and metallothionein concentration (Fig.…”

Section: Concentrations Of Metallothionein Zinc and Copper In Hepaticmentioning

confidence: 99%

Hepatic Metallothionein as a Source of Zinc and Cysteine during the First Year of Life

Zlotkin

Cherian

1988

Pediatr Res

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The use of cerenkov radiation in the measurement of115mcd in blood and other tissues

M.¹,

Bem²,

Reimschüssel³

1978

Bull. Environ. Contam. Toxicol.

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A new method for the activity measurement of 115mCd in biological samples was proposed. After tissues mineralization with 60% perchloric acid and 30% hydrogen peroxide (I) or with 0.5 M ethanolic sodium hydroxide (II) the solutions were measured in automatic liquid scintillation counter SL-30 by CERENKOV technique. The channels ratio method was successfully applied for standardization. The recoveries of 115mCd varied in the ranges: 93.6-102% and 82.7-99.3% for method I and II, respectively.

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Liver cadmium uptake, catalase inhibition and cadmium thionein production in the killifish (Fundulus Heteroclitus) induced by experimental cadmium exposure

Pruell

Engelhardt

1980

Marine Environmental Research

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A simple method for acid-extraction of cadmium from tissue

Cited by 14 publications

References 3 publications

Hepatic Metallothionein as a Source of Zinc and Cysteine during the First Year of Life

Hepatic Metallothionein as a Source of Zinc and Cysteine during the First Year of Life

The use of cerenkov radiation in the measurement of115mcd in blood and other tissues

Liver cadmium uptake, catalase inhibition and cadmium thionein production in the killifish (Fundulus Heteroclitus) induced by experimental cadmium exposure

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