A simple method for benzoyl chloride derivatization of biogenic amines for high performance liquid chromatography

Abstract

A simple, economical and reproducible benzoylation procedure was developed for the derivatization of biogenic amines (BAs) prior to high-performance liquid chromatography determination. The significant factors affecting biogenic amine benzoylation yield were optimized by central composite design. The derivatized BAs were extracted from basic aqueous solutions by 2-undecanol for direct HPLC injection. The obtained optimal conditions significantly decreased reagent consumption, uncertainty and time of analysis. The optimized method was used for further determination of BAs in real samples of nonalcoholic beers. The method showed good linearity (correlation coefficients > 0.997) and good recoveries (from 87.3 to 96.3%). The repeatability and reproducibility of the method were >4.6% and >6.7% respectively. Moreover, the detection limits of BAs were calculated between 0.02 and 0.09 mg ml À1 in non-alcoholic beers samples.

Benzoyl chloride derivatization with liquid chromatography–mass spectrometry for targeted metabolomics of neurochemicals in biological samples

“…Indeed, it has previously been used with MS or ultraviolet absorption detection for monitoring neurochemicals in dialysate [9, 10], plasma [10], and human cerebrospinal fluid (CSF) [11]. It has also been used for other amine [12, 13] and alcohol [14, 15] containing compounds. These previous assays targeted a relatively narrow group of compounds.…”

One drop chemical derivatization – DESI‐MS analysis for metabolite structure identification

“…Benzoylation with benzoyl chloride is usually performed in a biphasic reaction medium with an aqueous phase containing a base such as sodium hydroxide . Biphasic reactions can, however, not be performed in a drop size medium.…”

Biogenic Amines in Alcohol-Free Beverages

“…In the literature there are few studies carried out to evaluate BA profile and amount in these drinks. The beverages analyzed in the literature are orange carbonated-based drink [79], non-alcoholic beer [102,103], dairy beverages [104], and milk chocolate [105]. The BA determination methods used in soft drinks is high performance liquid chromatography, supported by UV [79,102,104,105]; FD [102,103] detector.…”

“…The analysis time for the reported literature methods varied between 35 and 71 min. In addition, the LOD and LOQ were 0.003-1.40 mg/L and 0.009-4.60 mg/L, respectively [79,[102][103][104][105]. Vieira et al (2007) evaluated BA amount in 35 samples of orange carbonated-based drinks.…”

“…The amines most present were putrescine (0.31-1.43 mg/L), cadaverine (0.11-0.56 mg/ L), tryptamine (0.28-2.56 mg/L), spermine (0.13-0.72 mg/L), and spermidine (0.16-0.81 mg/L). Phenylethylamine, histamine, and tyramine were only found in some of the analyzed non-alcoholic beer samples (Table 6) [102,103]. Another type of soft drink considered is dairy beverages.…”