A simple method for biocatalyst immobilization using PVA‐based hydrogel particles

Fernandes, Pedro; Marques, Marco P. C.; Carvalho, Filipe

doi:10.1002/jctb.2080

Cited by 33 publications

(16 citation statements)

References 23 publications

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“…These lens‐shaped hydrogels, termed LentiKats, were used for successful immobilization of cells and enzymes, as reported in numerous studies 118–120. Fernandez et al121 immobilized inulinase in PVA particles using LentiKat Liquid followed by extrusion directly into PEG 600 which afforded an instantaneous gelation of PVA. The hydrogels remained stable throughout an extended period of time at elevated temperature (50 °C) indicating efficiency of PEG as a dehydrating and stabilization agent.…”

Section: Supramolecular Polymer Engineeringmentioning

confidence: 99%

Poly(Vinyl Alcohol) Physical Hydrogels: New Vista on a Long Serving Biomaterial

Alves

Jensen

Smith

et al. 2011

Macromolecular Bioscience

209

159

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Poly(vinyl alcohol), PVA, and physical hydrogels derived thereof have an excellent safety profile and a successful history of biomedical applications. However, these materials are hardly in the focus of biomedical research, largely due to poor opportunities in nano- and micro-scale design associated with PVA hydrogels in their current form. In this review we aim to demonstrate that with PVA, a (sub)molecular control over polymer chemistry translates into fine-tuned supramolecular association of chains and this, in turn, defines macroscopic properties of the material. This nano- to micro- to macro- translation of control is unique for PVA and can now be accomplished using modern tools of macromolecular design. We believe that this strategy affords functionalized PVA physical hydrogels which meet the demands of modern nanobiotechnology and have a potential to become an indispensable tool in the design of biomaterials.

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Section: Supramolecular Polymer Engineeringmentioning

confidence: 99%

Poly(Vinyl Alcohol) Physical Hydrogels: New Vista on a Long Serving Biomaterial

Alves

Jensen

Smith

et al. 2011

Macromolecular Bioscience

209

159

View full text Add to dashboard Cite

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“…PVA hydrogel is chemically and mechanically stable compared to many other hydrogels (Fernandes et al 2009). GeniaLab markets an improved PVAbased hydrogel called LentiKat ® Liquid, which through controlled drying and chemical stabilisation produces lens-shaped particles for biocatalyst entrapment (Fernandes et al 2009;Wilson et al 2004).…”

Section: Improving the Mechanical Stability Of Spherezyme Tmmentioning

confidence: 98%

Modification of Alcalase SphereZyme™ by entrapment in LentiKats^®to impart improved particle stability

Molawa

Jordaan

Limson

et al. 2013

Biocatalysis and Biotransformation

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SphereZymes ä are self-immobilised enzyme particles that are formed by cross-linking enzymes while in an emulsion. The resultant spherical particles can be isolated and applied in biocatalytic reactions.Modifi cation of the cross-linking procedure to utilise a polyamine polymer (polyethyleneimine), in conjunction with glutaraldehyde, enhanced the activity of Alcalase ® SphereZyme preparations towards both large (azocasein) and small substrates ( p -nitrophenyl acetate), resulting in 16.6% and 53.7% retention in the activity, respectively, after immobilisation. To improve the fi lterability of these particles, we attempted two strategies. One was to include rigid polyethyleneimine -silica particles in the SphereZymes, and the second was to entrap SphereZymes in rigid PVA hemispheres (LentiKats ® ). Both methods greatly enhanced the fi lterability and robustness of the SphereZymes, with the entrapped particles being the easiest to handle.

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“…The operational stability of the immobilized biocatalyst was assessed by carrying out consecutive batch runs, based on a methodology described elsewhere [21][22][23]. Reactions were performed in 2 ml Safe-Lock Eppendorf Microtubes (VWR International-Material de Laboratório, Lda, Carnaxide, Portugal) with magnetic stirring (400 rpm) at 50°C, filled with 1.0 ml of a 5.0% (w/v) inulin solution in 0.1 M acetate buffer, pH 5.0.…”

Section: Evaluation Of Operational Stability Of the Immobilized Biocamentioning

confidence: 99%

From Inulin to Fructose Syrups Using Sol–Gel Immobilized Inulinase

Santa¹,

Bernardino

Magalhães³

et al. 2011

Appl Biochem Biotechnol

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The present work aims to provide the basic characterization of sol-gel immobilized inulinase, a biocatalyst configuration yet unexploited, using as model system the hydrolysis of inulin to fructose. Porous xerogel particles with dimensions in slight excess of 10 μm were obtained, yielding an immobilization efficiency of roughly 80%. The temperature- and pH-activity profiles displayed a broader bell-shaped pattern as a result of immobilization. In the latter case, a shift of the optimal pH of 0.5 pH units was observed towards a less acidic environment. The kinetic parameters estimated from the typical Michaelis-Menten kinetics suggest that immobilization in sol-gel did not tamper with the native enzyme conformation, but on the other hand, entrapment brought along mass transfer limitations. The sol-gel biocatalyst displayed a promising operational stability, since it was used in more than 20 consecutive 24-hour batch runs without noticeable decay in product yield. The performance of sol-gel biocatalyst particles doped with magnetite roughly matched the performance of simple sol-gel particles in a single batch run. However, the operational stability of the former proved poorer, since activity decay was evident after four consecutive 24-hour batch runs.

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A simple method for biocatalyst immobilization using PVA‐based hydrogel particles

Abstract: BACKGROUND: The aim of this study was to evaluate the feasibility of enzyme immobilization in PVA particles through extrusion of LentiKat  Liquid in polyethylene glycol. Inulinase, with invertase activity for sucrose hydrolysis, was used as model system.

Cited by 33 publications

References 23 publications

Poly(Vinyl Alcohol) Physical Hydrogels: New Vista on a Long Serving Biomaterial

Poly(Vinyl Alcohol) Physical Hydrogels: New Vista on a Long Serving Biomaterial

Modification of Alcalase SphereZyme™ by entrapment in LentiKats^®to impart improved particle stability

From Inulin to Fructose Syrups Using Sol–Gel Immobilized Inulinase

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A simple method for biocatalyst immobilization using PVA‐based hydrogel particles

Abstract: BACKGROUND: The aim of this study was to evaluate the feasibility of enzyme immobilization in PVA particles through extrusion of LentiKat  Liquid in polyethylene glycol. Inulinase, with invertase activity for sucrose hydrolysis, was used as model system.

Cited by 33 publications

References 23 publications

Poly(Vinyl Alcohol) Physical Hydrogels: New Vista on a Long Serving Biomaterial

Poly(Vinyl Alcohol) Physical Hydrogels: New Vista on a Long Serving Biomaterial

Modification of Alcalase SphereZyme™ by entrapment in LentiKats®to impart improved particle stability

From Inulin to Fructose Syrups Using Sol–Gel Immobilized Inulinase

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Product

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Modification of Alcalase SphereZyme™ by entrapment in LentiKats^®to impart improved particle stability