A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene

Bravi, Cláudio M.; Lirón, Juan Pedro; Mirol, Patricia M.; Ripoli, María Verónica; Peral‐García, Pilar; Giovambattista, Guillermo

doi:10.1016/j.legalmed.2004.06.003

Cited by 44 publications

(35 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More reliable results can be obtained by DNA-based tests such as Random Amplified Polymorphic DNA (RAPD) [10,11], Amplified Fragment Length Polymorphism (AFLP) [12,13] and Random Fragment Length Polymorphism (RFLP) [14][15][16]. These allow a specific DNA profile to be produced for an individual animal.…”

Section: Conventional Methods and Their Limitationsmentioning

confidence: 99%

Forensic Wildlife Parts and their Product Identification and Individualization Using DNA Barcoding

Panday¹,

Jha²,

Thapa³

et al. 2014

TOFORSJ

View full text Add to dashboard Cite

Despite very strict wildlife protection acts in several countries and trading laws such as CITES, illegal trading and poaching of wildlife is still active mostly because of the use of their parts in Chinese medicinal products and ornaments. For successfully identifying wildlife and their parts, mitochondrial DNA sequencing is being used now-a-days. DNA bar coding using fragments of cytochrome b and cytochrome oxidase I genes are frequently utilized for mammalian species identification. This mini review describes some of the mtDNA sequences and STR markers used for mammalian species identification in the field of forensic wildlife along with the problems associated and their possible solutions.

show abstract

Section: Conventional Methods and Their Limitationsmentioning

confidence: 99%

Forensic Wildlife Parts and their Product Identification and Individualization Using DNA Barcoding

Panday¹,

Jha²,

Thapa³

et al. 2014

TOFORSJ

View full text Add to dashboard Cite

show abstract

“…Bravi et al (2004) amplified a fragment of cyt b to identify meat of cattle, horse, donkey, pig, sheep, dog, cat, rabbit, chicken, and human using universal primers and 3 restriction enzymes (AluI, HaeIII, and HinfI). Ahmed et al (2007) amplified a segment of cyt b gene (359 bp) followed by digestion with TaqI to differentiate between cattle's and buffalo's meat.…”

Section: Restriction Fragment Length Polymorphism (Pcr-rflp)mentioning

confidence: 99%

“…The cyt b gene has been completely partially or sequenced for identification of numerous different species of birds, mammals (Bravi et al, 2004;Andrzej and Kamila, 2005), fishes, amphibian and reptiles (Chow et al, 1993;Ram et al, 1996;Quinterio et al, 1998;Lindstrom, 1999;Parson et al, 2000) and also some invertebrates (Lee et al, 2009). In addition, Chikuni et al (1994); Matsunaga et al (1998);La Neve et al (2008) used cyt b gene sequence to identify of meats and meat products of red deer, roe deer, song thrush, pyrenean ibex, chamois, quail and sparrow.…”

Section: Pcr-sequencingmentioning

confidence: 99%

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

| Identification of animal species of origin in meat and meat products is a matter of great concerns such as religious, economical, legal as well as medical aspects. Thus, several analytical techniques have been suggested for the identification of meat species either in individual or in mixed samples to protect consumers from the fraudulent and bad habits of marketing. DNA-based techniques especially the techniques based on polymerase chain reaction (PCR) are recognized as the most appropriate methods employed for species identification in raw and processed meat. PCR techniques including randomly amplified polymorphic DNA (PCR-RAPD), restriction fragment length polymorphism (PCR-RFLP), PCR with species-specific primers, real-time PCR and PCR-nucleotide sequencing allow identification of meat species under different processing conditions. But the variability of DNA content on the level of species as well as target tissue make the DNA-based methods somewhat unsuitable for the quantification of exact percentages of different species in meat and meat products. For these reasons the proteomic approaches depending on identification of different peptide biomarkers has been developed and employed to give information on the different composition of food. To broad the knowledge about these technologies, this review is compiled in an attempt to provide an overview of the possible PCR-based analytical techniques that could help in identifying the meat species of origin in meat and meat products and threw the light on the identification of species specific peptide biomarkers by proteomic technologies as a new and attractive alternative that could overcome some of the limitations that faced DNAbased methods especially when used for meat exposed to intensive heating of processing as well as for meat mixtures.

show abstract

“…Only few studies concerning host preference patterns were conducted for identification of vertebrate species [3,12] . These studies were based on the analysis of mitochondrial cytochrome b (cytb) gene which has been widely used [13][14][15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

Establishment of a molecular tool for blood meal identification in Malaysia

Lah

Ahamad

Haron

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene

Cited by 44 publications

References 20 publications

Forensic Wildlife Parts and their Product Identification and Individualization Using DNA Barcoding

Forensic Wildlife Parts and their Product Identification and Individualization Using DNA Barcoding

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Establishment of a molecular tool for blood meal identification in Malaysia

Contact Info

Product

Resources

About