A Simple Method for Extraction and Purification of Hypericins from St John’s Wort

Abstract

Hypericin was extracted from Hypericum perforatum L. using a different extraction procedure. Highest amounts of hypericins were extracted by ultrasonic methods using methanol: acetone 2:1 as an extracting solvent. Hypericin contents of the extract was purified by passing it through 2 series of 70 × 5 mm glass columns, each filled with 800 mg of 35 -70 mesh silica gel, followed by elution with MeOH: acetone: CH2Cl2 (75:10:15). The extent of purification was determined by HPLC. The amount of purified hypericins, which was extracted from Hypericum perforatum, was 5.105 mg per gram dried plant leaves.

Effect of Extraction Conditions on the Phenolic Content and DPPH Radical Scavenging Activity of Hypericum perforatum L.

“…The Hypericum genus has approximately 500 species all over the World. H. perforatum has been generally studied due to its biological activities including antitumor, antimicrobial, antiviral, and antidepressant (Ramezani and Zamani, 2017). Hypericum species have been used in traditional medicine for skin injuries and burns in public, as well (Dresler et al, 2018).…”