A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot

Deshmukh, Vishal P.; Thakare, P. V.; Chaudhari, Uddhav S.; Gawande, Prashant A.

doi:10.2225/vol10-issue3-fulltext-5

Cited by 33 publications

(25 citation statements)

References 8 publications

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“…It has been reported that it is difficult to obtain intact DNA from tropical species because of the presence of secondary composites, particularly those in the phenolics class (Rath et al, 1998;Deshmukh et al, 2007). During the C. antisyphiliticus DNA extraction process, both viscosity and oxidation were observed in the samples, and this problem was solved using the protocol developed by Deshmukh et al (2007).…”

Section: Discussionmentioning

confidence: 99%

“…During the C. antisyphiliticus DNA extraction process, both viscosity and oxidation were observed in the samples, and this problem was solved using the protocol developed by Deshmukh et al (2007). The effectiveness of this protocol is related to the higher number of washings to which the samples were subjected.…”

Section: Discussionmentioning

confidence: 99%

“…The first was proposed by Doyle and Doyle (1987), and the second was designed by Deshmukh et al (2007). Both methods were performed with modifications.…”

Section: Methodsmentioning

confidence: 99%

“…The protocol proposed by Deshmukh et al (2007) resulted in genetic material with the highest integrity as well as a mean DNA concentration of 393.43 ng/µL.…”

Section: Dna Extractionmentioning

confidence: 99%

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Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers

et al. 2016

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ABSTRACT. Croton antisyphiliticus Mart. is a medicinal plant native to Cerrado vegetation in Brazil, and it is popularly used to treat urogenital tract infections. The objective of the present study was to assess the genetic variability of natural C. antisyphiliticus populations using AFLP molecular markers. Accessions were collected in the states of Minas Gerais, São Paulo, and Goiás. The genotyping of individuals was performed using a LI-COR ® DNA Analyzer 4300. The variability within populations was found to be greater than the variability between them. The F ST value was 0.3830, which indicated that the populations were highly structured. A higher percentage of polymorphic loci (92.16%) and greater genetic diversity were found in the population accessions from Pratinha-MG. Gene flow was considered restricted (N m = 1.18), and there was no correlation between genetic and geographic distances. The populations of C. antisyphiliticus exhibited an island-model structure, which demonstrates the vulnerability of the species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The first was proposed by Doyle and Doyle (1987), and the second was designed by Deshmukh et al (2007). Both methods were performed with modifications.…”

Section: Methodsmentioning

confidence: 99%

“…The protocol proposed by Deshmukh et al (2007) resulted in genetic material with the highest integrity as well as a mean DNA concentration of 393.43 ng/µL.…”

Section: Dna Extractionmentioning

confidence: 99%

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Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers

et al. 2016

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“…1). These ramets along with mother leaf samples were subjected to genomic DNA extraction following extraction method described by Deshmukh et al (2007). Total DNA was quantified and its quality was verified by UV spectrophotometer (Cintra 404, Australia) and each sample was diluted to 40 ng per 3 ul with TE buffer and stored at 4°C.…”

Section: Introductionmentioning

confidence: 99%

Testing of genetic homogeneity of elite eucalyptus clones using DNA marker

Mohammad¹,

Dahayat²,

Pardhi³

et al. 2017

Trop. Plant Res.

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Under 'Hariyali Prasar Yojna' Chhattisgarh State Forest Department has procured and planted elite eucalyptus clones on large scale in different forest circles/divisions of the state during the monsoon of 2015-16 and 2016-17. To assess the genetic homogeneity of the supplied clones, genetic fidelity testing of the procured clones was carried out using ISSR marker. The monomorphic pattern of ISSR profiles observed for the ramets of the respective clones in comparison with their mother plant confirmed the genetic purity. This also demonstrates the application of molecular marker technology for quality control in social forestry plantation.

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Quantitative Study of As (V) and As (III) Interaction with Mangrove DNA by Molecular Fluorescence Spectroscopy

Majumder

Chowdhury

Ray

et al. 2014

Bull Environ Contam Toxicol

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This study describes the in vitro study of (1:1) one step nucleophilic displacement ([Formula: see text]) of phosphate by heavier anion arsenate and arsenite in the DNA of arsenic ridden Sundarban mangroves. Mangrove DNA was found to give rise to a broad fluorescence and its integrated fluorescence intensity was enhanced on addition of As (V) and As (III), respectively. Analyses of the fluorescence parameter showed adequacy of 1:1 model to describe substitution of phosphate of mangrove DNA chain exiplex by arsenate and arsenite with equilibrium constant (log Kc) ranging between 4.19 and 4.32 for As (V), and between 3.77 and 3.89 for As (III) at pH 7 and 25°C. In the cases, the melting temperature (Tm) and reassociation rate constant of mangrove DNA was increased on treatment with As (V) and As (III). It is suggested that heavier ion arsenate and arsenite may substitute phosphate in natural DNA.

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A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot

Cited by 33 publications

References 8 publications

Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers

Genetic diversity analysis of Croton antisyphiliticus Mart. using AFLP molecular markers

Testing of genetic homogeneity of elite eucalyptus clones using DNA marker

Quantitative Study of As (V) and As (III) Interaction with Mangrove DNA by Molecular Fluorescence Spectroscopy

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