A simple method for mitochondrial respiration and calcium uptake assessment in pollen tubes

Ortiz-Jiménez, Diana J.; López-Aquino, Casandra M.; Flores-Herrera, Cesar; Preciado-Linares, Gisela; Gonzalez-Vizueth, Israel; García-Arrazola, Roeb; Araiza‐Olivera, Daniela; Gutiérrez‐Aguilar, Manuel

doi:10.1016/j.mex.2019.07.023

Cited by 2 publications

(2 citation statements)

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“…Mitochondrial respiration was estimated using an Oxygraph (HansaTech) according to the method described by Diana [29]. Brie y, after calibration of the Oxygraph, pellets were resuspended in a nal volume of 0.5 mL into the Oxygraphs water-jacketed chamber containing PTA buffer with 10 mM sodium succinate, 0.01% digitonin and 200 mM ADP.…”

Section: Extraction Of Hippocampus Mitochondrial Matrixmentioning

confidence: 99%

Triggering Mitochondrial Dysfunction and Apoptosis Under Chronic Low-dose Lambda-cyhalothrin Exposure Modulated by Melissa Officinalis L Methanol Extract in Rat Hippocampus

Benaicha

Rouabhi

Gasmi

et al. 2021

Preprint

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The extensive use of Lambda-cyhalothrin (LCT) has been associated with the various toxicities that non-target organisms can undergo including mammals. However, the mechanism of induced-LCT cytotoxicity in animal brain cells is still elusive, particularly in brain regions, notably the hippocampus, an area directly involved in cognitive function. This study aimed to investigate the neurotoxic effects in the rat hippocampus chronically exposed to LCT (0.18 mg/kg and 0.36 mg/kg), and the neuroprotective potential of Melissa officinalis L methanol extract (MOE) (200 mg/kg) against this toxicity. After experimental period (90 days), the redox status, the functional and structural integrity of the hippocampus mitochondria as well as the apoptotic signaling pathway were evaluated. The current findings suggest that LCT produces imbalance of mitochondrial redox status expressed by, on one side, increasing of stress markers such as protein carbonyls (PCO), malondialdehyde (MDA), and hydrogen peroxide (H2O2) levels; and on the other side, by a decline in the potential of antioxidant systems, namely the level of mitochondrial enzymatic activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione (GSH).This study also showed an increase in mitochondrial permeability, along with mitochondrial edema and considerable decrease in its O2 consumption. Moreover, the same results recorded an increase in caspase-3 and cytosolic cytochrome-c. Conversely, this study proved that all these toxic aspects induced by LCT were significantly mitigated when the administration of this synthetic pyrethroid was associated with MOE. Taken together, data of this study shed light on mitochondrial damage and apoptosis stimulation under the toxic effect of LCT and suggests that MOE is endowed with potent neuroprotective effect, possibly via its antioxidant and antiapoptotic properties.

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Section: Extraction Of Hippocampus Mitochondrial Matrixmentioning

confidence: 99%

Triggering Mitochondrial Dysfunction and Apoptosis Under Chronic Low-dose Lambda-cyhalothrin Exposure Modulated by Melissa Officinalis L Methanol Extract in Rat Hippocampus

Benaicha

Rouabhi

Gasmi

et al. 2021

Preprint

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“…Calcium transients were assessed by measuring changes in the fluorescence of 2 μM Calcium Green-5N at 500 nm ex /530 nm em as previously reported. 37 Calcium transport was also assessed in the presence of 2 μM RuR to determine the potential contribution of MCUc to such transport activity.…”

Section: Methodsmentioning

confidence: 99%

An Arabidopsis mutant line lacking the mitochondrial calcium transport regulator MICU shows an altered metabolite profile

Gutiérrez-Mireles,

Páez-Franco,

Rodríguez-Ruíz

et al. 2023

Plant Signaling & Behavior

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Plant metabolism is constantly changing and requires input signals for efficient regulation. The mitochondrial calcium uniporter (MCU) couples organellar and cytoplasmic calcium oscillations leading to oxidative metabolism regulation in a vast array of species. In Arabidopsis thaliana , genetic deletion of AtMICU leads to altered mitochondrial calcium handling and ultrastructure. Here we aimed to further assess the consequences upon genetic deletion of AtMICU. Our results confirm that AtMICU safeguards intracellular calcium transport associated with carbohydrate, amino acid, and phytol metabolism modifications. The implications of such alterations are discussed.

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A simple method for mitochondrial respiration and calcium uptake assessment in pollen tubes

Abstract: Graphical abstract

Cited by 2 publications

References 4 publications

Triggering Mitochondrial Dysfunction and Apoptosis Under Chronic Low-dose Lambda-cyhalothrin Exposure Modulated by Melissa Officinalis L Methanol Extract in Rat Hippocampus

Triggering Mitochondrial Dysfunction and Apoptosis Under Chronic Low-dose Lambda-cyhalothrin Exposure Modulated by Melissa Officinalis L Methanol Extract in Rat Hippocampus

An Arabidopsis mutant line lacking the mitochondrial calcium transport regulator MICU shows an altered metabolite profile

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