A Simple Method for Obtaining Anaerobiosis

Weiss, Johanna; Spaulding, Earle H.

doi:10.1093/ajcp/8.ts2.1.36a

Cited by 15 publications

(14 citation statements)

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“…All samples were plated on 5% bovine blood agar and Eugon agar (BBL) and incubated aerobically and anaerobically according to the method described by Weiss and Spaulding (1937). A crystal violet stain was also made to determine the presence of spirochetes.…”

Section: Methodsmentioning

confidence: 99%

A Study of the Bacterial Flora of the Respiratory System of Normal Chickens

1958

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Section: Methodsmentioning

confidence: 99%

A Study of the Bacterial Flora of the Respiratory System of Normal Chickens

1958

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“…Anaerobic agar (BBL) plates were streaked and incubated according to the anaerobic method of Weiss and Spaulding (1937). Streak plates of each sample were made on Eugon agar (BBL), Tergitol 7 agar (BBL), Lactobacillus-Selection medium (BBL), Azide blood agar (BBL), and 5% Bovine blood agar and incubated aerobically.…”

Section: Methodsmentioning

confidence: 99%

Studies on “Air-Sac” Infection in Poultry

1960

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“…For single-colony isolation, no simple method has been devised, and one must use one of several cumbersome methods if anaerobic infection is suspected. The best method, which requires considerably less outlay than the original Mclntosh-Fildes or Navy methods, is that devised by Weiss and Spaulding,8 and Spaulding and Goode,9 in which a jar is evacuated, filled with carbon dioxide, then re-evacuated and filled with hydrogen, which removes the remaining oxygen in the presence of palladinized asbestos as a catalyst. A chemical method that works fairly satisfactorily is that of Rosenthal,10 in which chromium is added to sulfuric acid, first liberating hydrogen, and later removing the oxygen by an oxidation of the chromous compound formed.…”

Section: Smearsmentioning

confidence: 99%

Bacteriology

Janeway¹

1941

N Engl J Med

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WITH the development of powerful and specific agents for the treatment of infections, accurate diagnosis has become increasingly important. It is extremely difficult to handle a patient in diabetic coma without the aid of a chemical laboratory, and it is equally impossible to give satisfactory care to patients with serious infections without the assistance of a good bactériologie laboratory. Consequently, the efforts of the Massachusetts Department of Public Health to improve the quality of bactériologie work throughout the Commonwealth by a system of inspection and approval of laboratories is commended. With these considerations in mind, I shall discuss the present status of diagnostic procedures from the point of view of both the clinician and the laboratory man. Bacteriologic Procedures SmearsWith the refinement of laboratory methods, older and simpler tests are often forgotten. This has been true of the practice of examining stained smears of bacteriologic material. As a method of exact diagnosis, a smear has great limitations, but as a guide to the clinician and bacteriologist, it is of inestimable value. It gives a tentative indication of the type of infection in a few minutes, whereas cultures take at least eighteen hours, and it shows the proportions of various types of organisms in mixed infections, as well as the cellular reaction in the exúdate.Smears are particularly useful in the examination of exudates from sites normally sterile, such as serous cavities, the subarachnoid space and tissues. The importance of smear examination in cases of peritonitis and empyema cannot be overemphasized, for cultures from these cases often fail to grow. A discussion of the valuable information yielded by smears of abdominal fluid obtained at laparotomy has recently been published.1 Smears made from sites at which there are normally profuse bacterial flora are difficult to inter-prêt and usually fall to the lot of the medical man. Throat smears are extremely unreliable in the diagnosis of diphtheria and various forms of tonsillitis, and the physician should be guided by his clinical judgment from the appearance of the lesion and by properly taken cultures for confirmation.In pneumonia, the gross inspection of the sputum, in conjunction with examination of a stained smear, may yield valuable information, particularly in such unusual cases as Friedländer's pneumonia.In venereal diseases, smears may be very useful from a man, who normally has an almost sterile urethra, whereas those from the female cervix mean little unless frank pus is seen. Smears of urinary sediment are often more valuable than cultures, when the latter are done by simple broth inoculation. Perhaps the greatest use for smears in medical conditions is in examination of fluid aspirated from acutely inflamed joints, and of cerebrospinal fluid.Certain pitfalls in smear examination are not commonly appreciated. All the gram-positive cocci staphylococcus, pneumococcus and hemolytic streptococcus tend to assume a diplococcal form when growing rapidly in the ...

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A Simple Method for Obtaining Anaerobiosis

Cited by 15 publications

References 0 publications

A Study of the Bacterial Flora of the Respiratory System of Normal Chickens

A Study of the Bacterial Flora of the Respiratory System of Normal Chickens

Studies on “Air-Sac” Infection in Poultry

Bacteriology

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