A simple method for rapid purification of phycobiliproteins from Arthrospira platensis and Porphyridium cruentum biomass

Lauceri, Rosaria; Zittelli, Graziella Chini; Torzillo, Giuseppe

doi:10.1016/j.algal.2019.101685

Cited by 45 publications

(19 citation statements)

References 54 publications

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“…Scavenging activity of superoxide anion (%) = (1 − (A 1 /A 0 )) × 100% (7) where A 0 and A 1 are the absorbance of the control (prepared with distilled water) and the treatment samples, respectively.…”

Section: Superoxide Anion Radical Scavenging Activitymentioning

confidence: 99%

“…In 2021, the market price of 1 mg of highly pure (A 545 /A 280 > 5.0) B-phycoerythrin was USD 741.4 [ 6 ]. The common extraction methods for phycoerythrin include microwave-assisted extraction, in-situ stirring extraction, and the freezing/thawing method, which involves −20 °C freezing for 2 h, followed by thawing at room temperature (RT, 20–25 °C) [ 7 ]. Owing to the light and pH sensitivity, phycoerythrin extraction requires mild conditions [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…The crude phycoerythrin extract contains a large number of other proteins [ 7 ]. Similarly, the ethanol extract has impurity of fat-soluble small molecule compounds (e.g., triacylglycerol, chlorophyll, and carotenoids) [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae Porphyridium purpureum

Wang

et al. 2021

Foods

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The microalga Porphyridium accumulates high-value compounds such as phycoerythrin, polyunsaturated fatty acids, and polysaccharides, and thus, the extraction of these compounds could significantly expand the value of Porphyridium biomass. In the present study, a novel fractional extraction strategy based on the characteristics of these compounds was established using cold water, 95% ethanol, and hot water. The yield of phycoerythrin, lipids, and polysaccharides was 63.3, 74.3, and 75.2%, respectively. The phycoerythrin exhibited excellent fluorescence characteristics but had low purity. The crude lipid was dark with poor fluidity. Digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerol containing C20:5 and C20:4 were the most abundant glycerolipids, while glucose, xylose, and galactose constituted the intracellular polysaccharides that had covalently bound to proteins (8.01%), uronic acid (4.13%), and sulfate (8.31%). Compared with polysaccharides and crude lipids, crude phycoerythrin showed the best antioxidant activity. Overall, the three-step fractional extraction process was feasible for Porphyridium; however, further purification is necessary for downstream applications.

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Section: Superoxide Anion Radical Scavenging Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae Porphyridium purpureum

Wang

et al. 2021

Foods

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“…The following operations were performed as per Lauceri et al (2018Lauceri et al ( , 2019. Briefly, an equal amount of 30% Ult was mixed with 3 M AS and Buffer B to obtain 3 mL of phycobiliprotein mixture in different AS concentrations.…”

Section: Purification Of R-pcmentioning

confidence: 99%

“…At present, large-scale preparation of highpurity PBPs has not been realized (Mittal et al 2017). Lauceri et al (2019) used hydrophilic PVDF membranes as stationary phases and ammonium sulfate solutions of different concentrations as mobile phases to separate and purify allophycocyanin, phycocyanin and B-phycoerythrin from aqueous extracts of fresh or freeze-dried biomass of Arthrospira platensis (Cyanobacteria) and Porphyridium cruentum (Rhodophyta). The PVDF membrane system used in our method can be reused, expanded, and thus can be applied for a large-scale production, which provides a new method for the large-scale production of R-PC and R-PE from P. yezoensis.…”

Section: Introductionmentioning

confidence: 99%

Preparation of high-purity R-phycoerythrin and R-phycocyanin from Pyropia yezoensis in membrane chromatography

Zang

Qin

et al. 2020

J Appl Phycol

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R-phycoerythrin and R-phycocyanin are the main light-harvesting pigment-protein complexes in Pyropia yezoensis. They have bright colors and a high degree of fluorescence, and are now widely used in biomedicine, food, and cosmetic industries. Therefore, membrane chromatography was used to isolate and purify phycobiliprotein from P. yezoensis. Algal cells were disrupted by repeated freeze-thaw cycles. Then ammonium sulfate was added into the crude phycobiliprotein extract, so that the saturation of ammonium sulfate in the solution reached 20%, 30%, and 40% in sequence. At 30% and 40% ammonium sulfate saturation, precipitates of R-phycoerythrin and R-phycocyanin mixture in different proportions were obtained. The precipitates were ultra-filtrated to remove the salt, and then, the retentate was taken for membrane chromatographic treatment. In the membrane chromatographic treatment, salt-sensitive PVDF (polyvinyl difluoride) membranes were used for the follow-up experiments. By adjusting the concentration of ammonium sulfate, the membrane system changed the binding strength of various proteins, and then separated different types of phycobiliproteins. The final products of R-phycoerythrin (A λmax /A 280 purity 4.25, yield 45%) and R-phycocyanin (two purity grades: purity 5.8, yield 14.70% and purity 4.18, yield 39.89%) were obtained and then characterized by spectrophotometric and fluorometric measurements and SDS-PAGE. However, the large-scale production of R-phycocyanin and R-phycoerythrin remains a bottleneck as the column chromatography is difficult to be applied for industrial production. This method avoids the problem of easy clogging of column chromatography and can be useful for mass-production of R-phycoerythrin and R-phycocyanin in the future based on the laboratory results.

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The emergence and dominance of Planktothrix rubescens as an hypolimnetic cyanobacterium in response to re‐oligotrophication of a deep peri‐alpine lake

Suarez

Ventura²,

Stöckli³

et al. 2023

Limnology & Oceanography

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Toxic cyanobacteria, such as Planktothrix rubescens, came to dominate temperate lakes in the mid‐to‐late 20th century, as a result of eutrophication. Even after decades of re‐oligotrophication, where phosphorus levels were reduced by 1–2 orders of magnitude, P. rubescens remains present in various lakes. In this study, we examine the persistence and changes of P. rubescens deep chlorophyll maximum (DCM) in Lake Hallwil (Switzerland) over 35 yr of steadily decreasing phosphorous concentrations. Although lake transparency increased and the euphotic layer deepened during this period, the P. rubescens population maximum moved even deeper. It is now found ca. 7.7 m deeper than its shallowest position in the 2000s, and this depth no longer coincides with the depth of maximal water column stability. P. rubescens neutral buoyancy has now driven it beneath the stable metalimnion into the hypolimnion, where buoyancy regulation is restricted due to reduced metabolic activity at low light and low temperature. If P. rubescens DCM continues to deepen each year, it will eventually reach a region of lower stability in the hypolimnion where turbulent conditions are strong enough to disperse the DCM. We also explore the mechanisms that ensure P. rubescens ongoing presence in peri‐alpine lakes despite strong re‐oligotrophication and ongoing climate change. We find that P. rubescens in the lake is mainly sustained by growth during fully mixed conditions in winter, not during stratification in summer. This may contradict another commonly made prediction that periods of longer stratification will promote future blooms of this cyanobacterium.

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A simple method for rapid purification of phycobiliproteins from Arthrospira platensis and Porphyridium cruentum biomass

Cited by 45 publications

References 54 publications

A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae Porphyridium purpureum

A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae Porphyridium purpureum

Preparation of high-purity R-phycoerythrin and R-phycocyanin from Pyropia yezoensis in membrane chromatography

The emergence and dominance of Planktothrix rubescens as an hypolimnetic cyanobacterium in response to re‐oligotrophication of a deep peri‐alpine lake

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