A simple method for separating cells of<i>Microcystis aeruginosa</i>for counting

Humphries, Stella E.; Widjaja, Fifi

doi:10.1080/00071617900650331

Cited by 42 publications

(22 citation statements)

References 3 publications

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“…Cell numbers were calculated with the regression equation between algae density (×10 6 cell/mL) and A 680 as follows: cell number (×10 6 cells/mL)=40.325 A 680 −0.497 (n=14, R 2 = 0.997). This relationship was established by counting the cells under a microscope with a hemocytometer (Humphries and Widjaja 1979) and by determining absorbance (A 680 ) during the stationary phase of M. aeruginosa from the control (Zhu et al 2012).…”

Section: Measurement Of Cell Numbersmentioning

confidence: 99%

Effects of linear alkylbenzene sulfonate on the growth and toxin production of Microcystis aeruginosa isolated from Lake Dianchi

Wang

Zhang

Song

et al. 2014

Environ Sci Pollut Res

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The exogenous organic pollutant linear alkylbenzene sulfonate (LAS) pollution and Microcystis bloom are two common phenomena in eutrophic lakes, but the effects of LAS alone on Microcystis remain unclear. In the present study, we investigated the effects of LAS on the growth, photochemical efficiency, and microcystin production of Microcystis aeruginosa in the laboratory. Results showed that low LAS (≤10 mg/L) concentrations improved the growth of M. aeruginosa (12 days of exposure). High LAS (20 mg/L) concentrations inhibited the growth of M. aeruginosa on the first 8 days of exposure; afterward, growth progressed. After 12 days of exposure, the concentrations of chlorophyll a in algal cells were not significantly affected by any of LAS concentrations (0.05 to 20 mg/L) in the present study; by contrast, carotenoid and protein concentrations were significantly inhibited when LAS concentrations reached as high as 20 mg/L. After 6 and 12 days of exposure, low LAS (≤10 mg/ L) concentrations enhanced the maximal photochemical efficiency (Fv/Fm) and the maximal electron transport rate (ETRmax) of M. aeruginosa. Furthermore, LAS increased the microcystin production of M. aeruginosa. Extracellular and intracellular microcystin contents were significantly increased after M. aeruginosa was exposed to high LAS concentrations. Our results indicated that LAS in eutrophic lakes may increase the risk of Microcystis bloom and microcystin production.

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Section: Measurement Of Cell Numbersmentioning

confidence: 99%

Effects of linear alkylbenzene sulfonate on the growth and toxin production of Microcystis aeruginosa isolated from Lake Dianchi

Wang

Zhang

Song

et al. 2014

Environ Sci Pollut Res

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“…The determination of changes of filament length, the vegetative cell numbers between two heterocysts, and the heterocyst frequency was performed using a plankton counting chamber [27] under a compound light microscope (BX 51, Olympus, Tokyo, Japan). For each sub-sample, approximately 700-800 filaments were examined in order to measure the filament length and count the vegetative cell numbers between two heterocysts, and approximately 10,000-12,000 cells were counted for heterocyst frequency determination.…”

Section: Morphological Observationmentioning

confidence: 99%

Enhanced Resistance to UV-B Radiation in Anabaena sp. PCC 7120 (Cyanophyceae) by Repeated Exposure

Qin

2014

Curr Microbiol

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In natural habitats, organisms especially phytoplankton are not always continuously subjected to ultraviolet-B radiation (UVBR). By simulation of the natural situation, the N 2 -fixing cyanobacterium Anabaena sp. PCC 7120 was subjected to UV-B exposure and recovery cycles. A series of morphological and physiological changes were observed in Anabaena sp. PCC 7120 under repeated UVBR when compared with controls. Such as the breakage of filaments, intervals between heterocysts, heterocyst frequency, total carbohydrate, and carotenoids were increased, while the nitrogenase activity and photosynthetic activity were inhibited by repeated UVBR; however, these activities could recover when UV-B stress was removed. Unexpectedly, the over-compensatory growth was observed at the end of the second round of exposure and recovery cycle. Our results showed that discontinuous UVBR could increase the growth rate and the tolerance as well as repair capacity of Anabaena sp. PCC 7120. These results indicate that moderate UVBR may increase the growth of cyanobacteria in natural habitats.

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“…In order to count algal cells conveniently and accurately using the microscope, cells, particularly colonial Microcystis, were separated by sonication (LD-150N, Lede Technology Co., Ltd., Kunming, China) (Humphries and Widjaja 1979). The average cell numbers were counted by using a light microscope at ×4000 magnification (CX41, Olympus, Tokyo, Japan) with a plankton counting chamber and used to calculate the percent of the biomass.…”

Section: Phytoplankton Sample Collection and Analysismentioning

confidence: 99%

Fenced cultivation of water hyacinth for cyanobacterial bloom control

Qin

Zhang

Liu

et al. 2016

Environ Sci Pollut Res

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To achieve the goals of harmful cyanobacterial bloom control and nutrient removal, an eco-engineering project with water hyacinth planted in large-scale enclosures was conducted based on meteorological and hydrographical conditions in Lake Dianchi. Water quality, cyanobacteria distribution, and nutrient (TN, TP) bioaccumulation were investigated. Elevated concentrations of N and P and low Secchi depth (SD) were relevant to large amount of cyanobacteria trapped in regions with water hyacinth, where biomass of the dominant cyanobacteria Microcystis (4.95 × 10 10 cells L −1) was more than 30-fold compared with values of the control. A dramatic increase of TN and TP contents in the plants was found throughout the sampling period. Results from the present study confirmed the great potential to use water hyacinth for cyanobacterial bloom control and nutrient removal in algal lakes such as Lake Dianchi.

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A simple method for separating cells ofMicrocystis aeruginosafor counting

Cited by 42 publications

References 3 publications

Effects of linear alkylbenzene sulfonate on the growth and toxin production of Microcystis aeruginosa isolated from Lake Dianchi

Effects of linear alkylbenzene sulfonate on the growth and toxin production of Microcystis aeruginosa isolated from Lake Dianchi

Enhanced Resistance to UV-B Radiation in Anabaena sp. PCC 7120 (Cyanophyceae) by Repeated Exposure

Fenced cultivation of water hyacinth for cyanobacterial bloom control

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