A simple method for short term cultures from small biopsies

Meiosis and spermatogenesis in bulls heterozygous for a presumptive 1/29 Robertsonian translocation

“…Six of the offspring, each with different dams (3 heterozygous males, 2 normal males and 1 normal female) were purchased for more extensive study. Confirmation of their chromosome complements was sought by culturing fibroblasts (Basrur, Basrur & Gilman, 1963).…”

The Chromosomes of the Japanese Serow, Capricornis crispus (Temminck)

“…Biopsies were maintained in sterile tissue culture fluid to which antibiotics were added and processed several days later by the method originally published by Basrur et al (1963). Chromosome preparations were made in the conventional manner; after subculturing the explants they were stained with carbof uchsin and also with quinacrine hydrochloride and the trypsin-Giemsa technique (Seabright, 1972).…”

Studies on a male eland x kudu hybrid

“…These were grown according to the method described by Basrur et al (1963), and chromosome preparations were made in the usual manner. They were stained with Giemsa solution after pretreatment with trypsin: the flamed slides were treated for 2 hr in two changes of SSC at 60°C; washed in 0-5 % trypsin solution for 30 sec; washed briefly in 95 % alcohol; stained for 5 min in Giemsa solution (3 ml Giemsa/70 ml water); and finally washed again.…”