A Simple Method for the Quantitation of Haemoglobin Fractions Obtained by Starch-Gel Electrophoresis

Ricco, G; Gallo, Erika Alejandra Giraldo; Fiorina, L; Prato, V.

doi:10.1159/000209030

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…Haemoglobin electrophoresis was carried out by means of starch gel electrophoresis according to Smithies (1959) and each component was measured by the method of Ricco et al(1967). Fingerprints were made as summarized by Sick et a1 (1967); aminoacid analysis was performed in an EEL automatic analyser.…”

Section: A a T O Et A1mentioning

confidence: 99%

Haemolytic Anaemia due to Haemoglobin Torino

et al. 1970

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Haematological and clinical features of the haemolytic anaemia due to haemoglobin Torino are reported. The haematological picture is similar to that observed in other haemolytic anaemias due to unstable haemoglobins. The disease, present in three members of the same family, is inherited as a Mendelian co-dominant, non-sex-linked character.The disease is caused by the presence of haemoglobin Torino in erythrocytes and sulphonamide can provoke haemolytic crises. Besides the globin abnormality a decrease in porphobilinogen synthesis was demonstrated. Splenectomy improves the haematological state and clinical course.A new unstable haemoglobin, Hb Torino (a43 (CD I) phenylalanine-tvaline), has been recently described by Beretta et al (1968) in a man of Italian origin suffering from moderate anaemia with severe haemolytic crises. The clinical and haematological picture of the patient and of his relatives are described in this paper. Porphyrin synthesis and metabolism are also investigated in order to study the origin of the dipyrrolic urinary pigments excreted during the haemolytic crises. MATERIALS AND METHODSThe propositus, suffering from haemolytic anaemia due to Hb Torino, and 13 of his relatives belonging to three different generations were examined. Standard methods were used for haematological and biochemical investigations. Methaemoglobin was determined in fresh blood samples and in samples incubated for 48 hr at 37°C according to Evelyn & Malloy (1938); heat denaturation test was performed according to Dacie et a1 (1964); Heinz body induction test was carried out according to Beutler et a1 (1955) ; the methaemoglobin reduction test according to Brewer et a2 (1962) ; glutathione was measured using the method of Selwyn

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Section: A a T O Et A1mentioning

confidence: 99%

Haemolytic Anaemia due to Haemoglobin Torino

et al. 1970

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“…The technique of Ricco et at. [14] was used to evaluate the fractions. Haemoglobin fractions were chromatographically separated on Sephadex DEAE A-50 (2.5 cm X 100 cm) anionic resin exchange column, using a pH gradient buffered from 8.1 (tris-HCl 0.05 m) to 7.0 [8], After vacuum concentration, a-and /5-chains were separated by incubation with parachloromercuribenzoate (PCMB) and examined by starch gel electrophoresis at pH 8.3 [15].…”

Section: Methodsmentioning

confidence: 99%

A Case of Haemoglobin J Sardegna/<i>β</i>-ThaIassaemia Double Heterozygosis

Gallo¹,

Pugliatti²,

Ricco³

et al. 1972

Acta Haematol

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Clinical and haematological findings of a Sardinian family are described. Three members were carriers of Hb J Sardegna, 5 were heterozygous for β-thalassaemia and 2 were double heterozygous for Hb J Sardegna and β-thalassaemia. The presence of Hb J Sardegna did not give rise to appreciable changes in the haematological picture: heterozygous carries displayed normal values, while double Hb J/β -thalassaemia heterozygotes presented clinical and haematological signs typical for β -thalassaemia. In the propo-situs, the relative rate of synthesis of «- and β-chains and oxygen dissociation curve were studied. The interaction between β -thalassaemia and α-chain variants is discussed.

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“…The quantitation of each fraction was carried out according to Ricco el al. [6]. For electrophore sis, fresh haemoglobin, treated with potassium cyanide immediately before use, was utilized, to avoid the formation of slow moving components which tend to migrate into the same position as normal foetal haemoglobin.…”

Section: Methodsmentioning

confidence: 99%

“…The authors who have dealt with the problem of the screen ing of thalassaemia agree in recognizing that none of these tests by itself enables one to detect unmistakably all the carriers [2,3,5,9]. A simple method has recently been perfected which allows one to dose accurately all the haemoglobin fractions obtained by starch gel electro phoresis [6]. By this method the value of each haemoglobin fraction in a sufficiently large number of thalassaemia heterozygous patients has been determined; these values were compared with those drawn from a large number of clinically and haematologically normal subjects and with those of subjects suffering from haematological disorders of different kinds, in order to establish whether the quantitation of the haemoglobin fractions obtained by means of starch gel electrophoresis can profitably be used, even alone, in the screening of thalassaemia.…”

Section: The Starch Gel Quantitative Electrophoresis Of Haemoglobin Amentioning

confidence: 99%