A Simple Method for the Determination of Pharmaceutical and Personal Care Products in Fish Tissue Based on Matrix Solid-Phase Dispersion

Abstract

A simple and effective pretreatment method based on matrix solid-phase dispersion was developed for the determination of pharmaceutical and personal care products (PPCPs) and their metabolites in fish by high-performance liquid chromatography tandem mass spectrometry. The type and amount of dispersant, adsorbent, and eluting solvent were optimized by a single-factor experiment and Box−Behnken design. Under the optimal conditions with 2.5 g of Florisil as a dispersant, 500 mg of C 18 as an adsorbent, and 5 mL of acetonitrile as an eluting solvent, the recoveries ranged from 70.4 to 99.9% with relative standard deviations less than 10.5%, and the limits of quantitation ranged from 0.13 to 1.01 μg/kg. The developed method was successfully applied to detect PPCPs in marketed fish, and five PPCPs, including triclocarban, sulfadiazine, sulfadimidine, sulfamethoxazole, and carbamazepine, were detected at trace levels. The proposed method, which has the advantages of short analysis time, less solvent consumption, and high sensitivity, can be used for the determination of trace PPCPs in fish.

Tunable Fluorine-Functionalized Scholl-Coupled Microporous Polymer for the Selective Adsorption and Ultrasensitive Analysis of Environmental Liquid-Crystal Monomers

“…The ranges of three independent variables were determined via the path of the steepest ascent method (Figure S16B−D). 44 The regression model was described by a second-order polynomial function, and its quality was evaluated by analysis of variance (ANOVA). The ANOVA analysis of the fitted second-order polynomial model determined a p-value <0.0001 (Table S7), which demonstrated that the established model was statistically significant.…”