A simple method for the preparation of an affinity absorbent for soybean agglutinin using galactosamine and CH‐sepharose

doi:10.1016/0014-5793(75)90068-x

FEBS Letters

1975

DOI: 10.1016/0014-5793(75)90068-x

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A simple method for the preparation of an affinity absorbent for soybean agglutinin using galactosamine and CH‐sepharose

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1985

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Cited by 43 publications

References 11 publications

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Isolation of the acidic and basic lectins from winged bean seed (Psophocarpus tetragonolobus (L.) DC)

Kortt

Caldwell

1985

J Sci Food Agric

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The acidic and basic lectins of the winged bean were isolated from seed extracts by affinity chromatography on lactose-Sepharose 6B and melibiose Bio-Gel P150, respectively. The lectins isolated from six varieties of Psophocarpus tetragonolobus, collected from different regions of south-east Asia, showed no significant differences in their properties; their subunit M, and amino acid compositions were the same as those of the acidic and basic lectins isolated by non-affinity methods. On isoelectric focusing the affinity purified acidic lectins showed two major protein components (isolectins) while the affinity purified basic lectins had four protein components. The acidic lectins showed a preference for b-D-galactosides and they agglutinated trypsinised human type A, B and 0 erythrocytes but not trypsinised rabbit erythrocytes. The basic lectins showed a preference for a-D-galactosides and they agglutinated trypsinised rabbit and human type A and B erythrocytes but not trypsinised human type 0 erythrocytes.

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Isolation of the acidic and basic lectins from winged bean seed (Psophocarpus tetragonolobus (L.) DC)

Kortt

Caldwell

1985

J Sci Food Agric

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Isolation of an N-acetyl-d-galactosamine-binding protein from winged bean (Psophocarpus tetragonolobus)

Appukuttan

1981

Analytical Biochemistry

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Derivatization of epoxy-activated agarose with various carbohydrates for the preparation of stable and high-capacity affinity adsorbents: Their use for affinity chromatography of carbohydrate-binding proteins

Matsumoto

Kitagaki

Akai

et al. 1981

Analytical Biochemistry

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A simple method for the preparation of an affinity absorbent for soybean agglutinin using galactosamine and CH‐sepharose

Cited by 43 publications

References 11 publications

Isolation of the acidic and basic lectins from winged bean seed (Psophocarpus tetragonolobus (L.) DC)

Isolation of the acidic and basic lectins from winged bean seed (Psophocarpus tetragonolobus (L.) DC)

Isolation of an N-acetyl-d-galactosamine-binding protein from winged bean (Psophocarpus tetragonolobus)

Derivatization of epoxy-activated agarose with various carbohydrates for the preparation of stable and high-capacity affinity adsorbents: Their use for affinity chromatography of carbohydrate-binding proteins

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