A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates

Sierra, Gonzalo

doi:10.1007/bf02545855

Cited by 806 publications

(410 citation statements)

References 12 publications

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“…The isolates were inoculated in the media which is described by Sierra [56], containing (g/l) peptone 10, NaCl 5, CaCl2 2H2O 0.1, agar-agar 18, and 1% of sterilized tween 80. After incubation at 30°C for 48 h a clear halo around the colonies demonstrated esterase activity.…”

Section: Esterase Activitymentioning

confidence: 99%

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Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Bensidhoum

Nabti

Tabli

et al. 2016

European Journal of Soil Biology

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The application of plant growth-promoting bacteria (PGPB) with biocontrol activities as inoculants of crops plants against phytopathogenic fungi and insect pests provides a biological alternative to the use of agrochemicals. Two Pseudomonas sp. strains were isolated from agricultural well water in the area of Bejaia, northeastern Algeria, located rather closely to a lead mine deposit. The isolates S4LiBe and S5LiBe had 16S rRNA gene sequence similarities of 99.4% to 99.7% with Pseudomonas protegens CHAO T and other P. protegens strains. The phenotypic profiles tested with BIOLOG-GN2-microplates were very similar, but showed also some remarkable differences. The isolates S4LiBe and S5LiBe showed plant growthpromoting potential based on the production of the phytohormone indole acetic acid and siderophores and the solubilization of insoluble phosphate. In addition, they produced chitinase and other polymer degrading enzymes. Interestingly, while S4LiBe and S5LiBe were resistant against heavy metals (2.0 mM K2Cr2O7 and 3.0 mM CoSO4, HgSO4, CdSO4 8H2O and PbCl2), the reference strain P. protegens CHAO T was very sensitive to Hg 2+ and Cd 2+ and had lower tolerance towards Co 2+ and Pb 2+ . The isolates S4LiBe and S5LiBe were very active in mycelial growth inhibition assays against Botrytis cinerea, Verticillium dahlia, Fusarium graminearum, Aspergillus niger and A. flavus (growth inhibition between 88% and 48%). Furthermore, S4LiBe and S5LiBe showed effective insecticidal activities, when tested in the Galleria injection assay and they were tested positive for the insect toxin gene fitD alike the reference strain CHA0 T . Finally, inoculation of barley seeds with S5LiBe resulted in significantly stimulated germination rate and growth of seedlings, with increased shoot length, shoot and root fresh weight, shoot and root dry weight as compared to non-inoculated plants.Thus, the heavy metal tolerant isolates S4LiBe and S5LiBe harbor a diverse potential as beneficial bacteria for agricultural application. They may be very useful even in polluted soils for the stimulation of e.g. biomass crops. The demonstration of successful isolation from agricultural well water may open more ready access for a wide variety of this kind of beneficial bacteria for agricultural application.

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Section: Esterase Activitymentioning

confidence: 99%

“…Lipolytic activity was determined as described above for the determination of esterase activity [56]. In this experiment, tween 80 was replaced by tween 20.…”

Section: Lipolytic Activitymentioning

confidence: 99%

Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Bensidhoum

Nabti

Tabli

et al. 2016

European Journal of Soil Biology

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“…Allantoin hydrolysis was evaluated by using the same basal medium suggested by Gordon et al for esculin hydrolysis. Phosphatase activity was evaluated by using the method of Kurup and Schmitt (€9, and Tween hydrolysis was evaluated by the method of Sierra (19). The temperature range for growth was determined on slants of ATCC medium 172 agar, and antibiotic susceptibility was determined by using BBL Microbiology Systems disks on ATCC medium 172 plates swabbed with cell suspensions of the strains.…”

Section: Methodsmentioning

confidence: 99%

Kitasatosporia mediocidica sp. nov.

Labeda

1988

International Journal of Systematic Bacteriology

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A study of two similar actinomycete strains, NRRL B-16109 and NRRL B-16110, demonstrated the presence of both the meso and LL isomers of diaminopimelic acid in whole-cell hydrolysates, typical of the genus Kitasatosporiu Omura et al. These strains also had a phospholipid composition (type PII; diagnostic constituent, phosphatidylethanolamine) typical of this genus. Studies of deoxyribonucleic acid relatedness between these strains and the type strains of the three previously recognized species of Kitusatosporia showed that they are not closely related to the other species. A new species, Kitusutosporiu mediocidicu, is proposed for these novel strains, and NRRL B-16109 is designated the type strain.During a study of the biochemical characteristics and deoxyribonucleic acid (DNA) relatedness of strains presumptively considered members of the genus Saccharothrix based on the presence of meso-diaminopimelic acid (DAP), rhamnose, and galactose in whole-cell hydrolysates, two strains were identified as phenotypically and genetically distant from all of the other strains studied but virtually identical to each other. These strains, NRRL B-16109T (T = type strain) and NRRL B-16110, initially came from the laboratory of L. McDaniels at the Waksman Institute and were of interest to him because they produced the antibiotic mediocidin. The origin of these strains was unavailable in the records of L. McDaniels. A subsequent reexamination of cell wall composition revealed the presence of both the LL and meso isomers of DAP in varying proportions and also a slight amount of glycine. This unique presence of both DAP isomers is diagnostic of members of the genus Kitasatosporia (5,14). These strains appear to be novel compared with previously described species in this genus and are described as the new species Kitasatosporia mediocidica. MATERIALS AND METHODSMaintenance of strains. The strains included in this study are summarized in Table 1. Strains were maintained on agar slants of ATCC medium 172 (NZamine-soluble starch-glucose agar) (1) and were stored at 4°C until needed. Inocula for the physiological tests and biomass for extraction of DNA were grown in ATCC medium 172 broth from which calcium carbonate was omitted.Morphological observations. Gross colonial observations were made of 14-day growth on the standard media suggested by the International Streptomyces Project (18), as well as ATCC medium 172. Spore chain morphology was observed and photographed by light microscopy, and spore surface properties were observed on unstained contact preparations by using a Hitachi model H-500 transmission electron microscope.Cell chemistry. Cell wall type and whole-cell sugar and phospholipid compositions were determined by previously described methods (2, 3, 9, 10, 20).Physiological tests. The physiological characteristics of the strains were evaluated by using the media of Gordon et al. (4). Allantoin hydrolysis was evaluated by using the same basal medium suggested by Gordon et al. for esculin hydrolysis. Phosphatase activity was e...

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“…Esculin hydrolysis was observed by the method of Sneath (15). Lipase was detected by the method of Sierra (13), and urease was tested in Christensen urea-agar medium (2). Table 1 shows translucens f.sp.…”

Section: Methodsmentioning

confidence: 99%

Differentiation of Xanthomonas translucens f.sp. oryzicola (Fang et al.) Bradbury, the Leaf-Streak Pathogen, from Xanthomonas oryzae (Uyeda and Ishiyama) Dowson, the Blight Pathogen of Rice, by Enzymatic Tests

Reddy

1974

International Journal of Systematic Bacteriology

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Isolates of Xanthomonas translucens f .sp. oryzicola readily produced phenylalanine deaminase 1 day after inoculation and tyrosinase and 0-glucosidase 4 days after inoculation, but isolates of Xanthomonas oryzae showed no such enzymatic activities by the methods employed. These differences easily distinguish bacterial blight of rice (caused by X. oryzae) from leaf streak of rice (caused by X . translucens f.sp. oryzicola).In the tropics the two most common bacterial diseases of the rice plant are blight and leaf streak. Bacterial blight, known for many All the authors mentioned failed to differentiate the pathogens from one another by physiological characteristics. This paper reports a study aimed at determining whether specific enzymatic tests can readily distinguish one pathogen from the other. MATERIALS AND METHODSBacterial cultures. Forty isolates of X. oryzae and 16 isolates of X. translucens f.sp. oryzicola were selected from the bacterial stock cultures of the Plant Pathology Department of the International Rice Research Institute, Manila. The cultures were maintained on Wakimoto (18) agar slants (potato, 300 g; Ca(N0, ), )*4H, 0, 0.5 g; Na, HPO, 12H, 0, 2.0 g; peptone, 5.0 g; sucrose, 15.0 g; agar, 20.0 g; distilled water, 1,000 ml; pH adjusted to 6.8 to 7.0) in a deep freezer at -30 C. Twenty-four-hour-old agar slant cultures were used in inoculating test media, and they were incubated at +28 C after inoculation.tained by the method of Thornley (17), and asparagine hydrolase was tested similarly with 1% L-asparagine, whereas arginine decarboxylase and phenylalanine deaminase were determined by using the procedures described by Skerman (14). Catalase was detected with 10 vol of H,O,, and oxidase was t e s t e d w i t h 1 5% N,N-d i m e t hyl-p-phenylenediammonium dichloride (Merck) according to Kovacs (9) and Ewing and Johnson (4). Similarly, tyrosinase was demonstrated with L-tyrosine (19) and 0-glucosidase with 0.5% arbutin (8) after 4 days of incubation. Esculin hydrolysis was observed by the method of Sneath (15). Lipase was detected by the method of Sierra (13), and urease was tested in Christensen urea-agar medium (2).Biochemical tests. Arginine hydrolase was ascer-

show abstract

A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates

Cited by 806 publications

References 12 publications

Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Kitasatosporia mediocidica sp. nov.

Differentiation of Xanthomonas translucens f.sp. oryzicola (Fang et al.) Bradbury, the Leaf-Streak Pathogen, from Xanthomonas oryzae (Uyeda and Ishiyama) Dowson, the Blight Pathogen of Rice, by Enzymatic Tests

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