A Simple Method for the Comparison of Commercially Available ATP Hygiene-Monitoring Systems

Colquhoun, K. O.; Timms, S.; Fricker, C. R.

doi:10.4315/0362-028x-61.4.499

Cited by 24 publications

(13 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Poulis et al (1993) noted that there was a poor relationship between ATP readings and conventional microbiological culture methods in samples from food processing facilities. Colquhoun et al (1998) compared three different ATP hygiene monitoring systems and found that they differed considerably in sensitivity and reproducibility.…”

Section: Article In Pressmentioning

confidence: 99%

Bioluminescence ATP monitoring as a surrogate marker for microbial load on hands and surfaces in the home

et al. 2003

View full text Add to dashboard Cite

To evaluate the validity and reliability of adenosine triphosphate (ATP) monitoring as a surrogate marker for cleanliness, the kitchen table surface in 225 inner-city homes was sampled by microbiologic culture and by two types of biomass monitoring systems (HY-LiTEt 2 ATP System and HY-RiSE Colour Hygiene Test Strip, EM Science, Gibbstown, New Jersey, USA). A randomly selected hand of the homemaker (n ¼ 225) was also cultured and sampled with the ATP monitoring system immediately after handwashing. Log microbial counts on hands ranged from 3.2 to 7.0 and from the table, 1.0 to 5.5. While the traditional ATP readings (HY-LiTE

show abstract

Section: Article In Pressmentioning

confidence: 99%

Bioluminescence ATP monitoring as a surrogate marker for microbial load on hands and surfaces in the home

et al. 2003

View full text Add to dashboard Cite

show abstract

“…E-mail: nandrade@ufv.br growth and metabolisms have been developed using principles of ATP-bioluminescence, biophysics, radiometry and impedance measurement. ATP-bioluminescence assay detects the ATP amount on the contact surfaces in food processing systems, as well as in food samples (4,5,6,12,16). The ATP detected by this technique is derived from microorganisms, somatic cells, plants and animals.…”

Section: Introductionmentioning

confidence: 99%

ATP-bioluminescence assay as an alternative for hygiene-monitoring procedures of stainless steel milk contact surfaces

Costa

Andrade

Brandão

et al. 2006

Braz. J. Microbiol.

View full text Add to dashboard Cite

ATP-bioluminescence assay was used to evaluate the cleaning and sanitizing procedures of stainless steel milk contact surfaces. Surfaces of a raw milk transportation tank, a raw milk cooling storage tank, an equalization pasteurizing tank, a pasteurized milk storage tank and a pasteurized milk packaging tank, as well as a milk centrifuge were evaluated. The Relative Light Unit (RLU) using a commercial luminometer and mesophilic aerobic counts (CFU.cm -2 ) on surfaces were determined. There were no significant differences (p>0.05) in RLU on the assessed surfaces before and after the cleaning and sanitizing procedures. Also, there were no significant differences (p>0.05) in CFU.cm -2 on the surfaces before the cleaning and sanitizing procedures, whereas different bacterial numbers were found on surfaces after the hygiene procedures. The surfaces of the milk centrifuge, raw milk cooling tank and milk packaging tank showed the largest mesophilic aerobic numbers after the hygiene, reaching 2.21, 2.18, and 1.21 log10, respectively. The results demonstrated that there is no agreement among the techniques for classification of hygienic conditions of the evaluated surfaces, showing a non-direct relation between RLU and CFU.cm . The ATP-bioluminescence method showed that 100% of the surfaces were under inadequate hygiene conditions, while the plate count method detected only 50%, based on the APHA's recommendation, and 33%, based on the WHO's recommendation. High variations in RLU measurements were observed, suggesting the need for more than only one surface analysis. The ATPbioluminescence is not a direct replacement for microbiological testing, but provides complementary information and should be used as part of an integrated strategy.

show abstract

“…4,5 Hygiene monitoring using ATP also offers a simple and valuable means of monitoring dental practice cleaning routines. 6 This test is relatively simple to perform and can be done at the site of sampling.…”

mentioning

confidence: 99%

Total viable counts, ATP, and endotoxin levels as potential markers of microbial contamination of dental unit water systems

Fulford¹,

Walker²,

Martin

et al. 2004

Br Dent J

View full text Add to dashboard Cite

Objectives To determine if either ATP or endotoxin concentrations in water supplied by dental unit water systems (DUWS) correlated with total viable counts (TVC), and therefore could be used as a rapid, chairside measure of levels of microbial contamination. Design A prospective trial. Method Fifty-seven water samples were taken from the 'triple spray', air rotor and source water supplies from 25 dental units in eight practices. The samples were assayed for endotoxin concentration, total ATP and TVC. A pilot study was performed to assess the relationship between TVC and total cell counts, as determined by flow cytometry. Results ATP concentrations ranged from 22 to 958 relative light units (RLU) and free endotoxin ranged from 25 to 600 EU ml -1 . TVC varied from not detected to 2.16 x 10 4 CFU ml -1 . The ATP method proved to be a simple and rapid method that could be used at the chairside. However, there was no correlation between ATP or endotoxin concentrations and TVC in DUWS. TVC generally underestimated the total cell count by 50 to 500 fold. Conclusion Half of the water samples from DUWS exceeded recommended levels of TVC. However, ATP and endotoxin concentrations in DUWS water samples did not correlate with these TVC data and therefore could not be recommended as an alternative assay to TVC for measuring bacterial contamination or for monitoring water treatment efficacy.Dental unit water systems (DUWS) are generally contaminated with bacteria. 7,9,13,15 The normal method of measuring bacterial contamination is by total viable counts (TVC) following culture on microbiological media in a dedicated laboratory. This is a time- consuming and labour intensive process, and there is an inevitable delay before the data are available and can be acted upon. In order for the bacterial counts to be as accurate as possible, it is essential to ensure that the samples are processed without delay after collection. This may prove difficult if the site of collection is remote from the microbiology laboratory.In the brewing and food industry, bacterial and other cellular contamination is estimated by measuring total adenosine triphosphate (ATP). 4,5 Hygiene monitoring using ATP also offers a simple and valuable means of monitoring dental practice cleaning routines. 6 This test is relatively simple to perform and can be done at the site of sampling. The simplicity and rapidity of the ATP test system are features that could potentially make this a feasible approach to measure contamination of DUWS at the chairside. This would be particularly useful in confirming the efficacy of decontamination procedures.Another potential method of estimating TVC could be to measure endotoxin concentration. Much of the bacterial contamination of DUWS is by Gram-negative bacteria, which on lysis would release endotoxin. Thus, endotoxin estimation could potentially also be used as a measure of bacterial contamination of DUWS. This assay is used extensively in the pharmaceutical industry for testing the production of endotoxin-free water.The purp...

show abstract

A Simple Method for the Comparison of Commercially Available ATP Hygiene-Monitoring Systems

Cited by 24 publications

References 0 publications

Bioluminescence ATP monitoring as a surrogate marker for microbial load on hands and surfaces in the home

Bioluminescence ATP monitoring as a surrogate marker for microbial load on hands and surfaces in the home

ATP-bioluminescence assay as an alternative for hygiene-monitoring procedures of stainless steel milk contact surfaces

Total viable counts, ATP, and endotoxin levels as potential markers of microbial contamination of dental unit water systems

Contact Info

Product

Resources

About